| Project/Area Number |
11793012
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Kobe University |
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Principal Investigator |
OHKAWA Hideo Kobe University, Research Center for Environmental Genomics, Professor, 遺伝子実験センター, 教授 (90233048)
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| Co-Investigator(Kenkyū-buntansha) |
KATAGIRI Masanao Osaka Kyoiku University, Division of Natural Science, Associate Professor, 教育学部, 助教授 (00185802)
INOUYE Kuniyo Kyoto University, Graduate School, Division of Food Science and Biotechnology, Professor, 大学院・農学系研究科, 教授 (10223249)
NAKATA Masanobu Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (40031190)
MIYAKE Shiro Bio Applied Systems Inc.,, 開発・営業部長(研究職)
KAWATA Mitsuyasu Otsuka Chemical Co., Ltd., Naruto Research Center, 鳴門研究所, 主任研究員
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥10,100,000 (Direct Cost: ¥10,100,000)
Fiscal Year 2001: ¥10,100,000 (Direct Cost: ¥10,100,000)
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| Keywords | ELISA / monoclonal antibody / scFv / endocrine disruptor / pesticide residues / synchronous enzyme reaction / monitoring / 環境健康影響物質 / ノニルフェノール / シメトリン / エンドスルファン代謝物 / ビスフェノールA / マラチオン / モノクロナール抗体 / エンドスルファン / トリフルラリン / 可変領域cDNA / バイオモニタリング |
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| Research Abstract |
Expansion of contamination of the environment and agricultural products with environmental health chemicals including dioxins, endocrine disruptors and pesticide residues is a serious global problem. These chemicals were found in the environment and agricultural products at ultra-low concentrations (ppt, ppb and ppb levels), and suspected of affecting ecosystems and human health. Therefore, it is important to develop novel technologies to monitor contamination by ultra-low concentrations of environmental health chemicals, since most conventional technologies are high cost, labor intensive and taking time to obtain results, and do not work effectively to monitor contamination of ultra-low chemicals. Therefore, development of novel technologies based on biological functions appears to be requested, because of the low risk potential for secondary contamination with chemicals. We prepared monoclonal antibodies specific to the endocrine disrupters bisphenol A, nonylphenol and estrogen, the insecticide malathion, and the herbicide cymethrin and then developed enzyme-linked immunosorbent assay (ELISA) based on the monoclonal antibodies. The ELISA methods developed were highly sensitive, convenient and low-cost as compared with the conventional analytical methods. We also developed recombinant scFv antibodies based on the monoclonal antibodies by genetic engineering and prepared affinity columms for clear-up of the extracts of samples of soil and agricultural products. In addition, a synchronous enzyme reaction system was developed for a highly sensitive ELISA. It was considered that standardization of these novel ELISA methods is important for practical use.
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