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Research and Development of Protective Compounds against UV Iight-induced Skin Damage on the Basis of anti-ROS properties

Research Project

Project/Area Number 11793015
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field Environmental pharmacy
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

SAKURAI Hiromu  Kyoto Pharmaceutical University, Professor, 代謝分析学, 教授 (30065916)

Co-Investigator(Kenkyū-buntansha) OKA Shigenori  Nagasesangyo, Co., Ltd., Research associate, ビューティーケア, 研究員
ONO Makoto  Nagasesangyo, Co., Ltd., Manager, ビューティーケア, 部長
YASUI Hiroyuki  Kyoto Pharmaceutical University, Instructor, 代謝分析学, 助手 (20278443)
田和 理市  京都薬科大学, 代謝分析学, 助教授 (80142587)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2001: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsUltraviolet ray / Reactive oxygen species / UV-induced skin damage / Chemiluminescence / Zinc(II) ion / Ascorbic acid derivatives / Caffeic acid derivatives / Hairless mouse / 皮膚障害 / フリーラジカル / 化学発光学 / 微弱光測定 / 抗酸化剤 / ラジカルスカベンジャー / 皮膚損害 / 化学発光法
Research Abstract

Recent increase of ultraviolet (UV) lights due to the increase of ozone hole is suggested to enhance a severe damage and premature photoaging in the skin of animals. Although the harmful effects of UV radiation on the skin are associated with the generation of reactive oxygen species (ROS), direct proof of ROS production in the skin under UV irradiation has been elusive. Recently, we have reported first an in vivo detection and imaging of the generated ROS in the skin of alive animals following UVA irradiation, where both a sensitive and specific chemiluminescent probe (CLA) and ultralow-light imaging apparatus with a CCD camera were used. Then, we proposed that superoxide anion radical (・O_2^-) is formed spontaneously and singlet oxygen (^1O_2) is generated in the UVA-irradiated skin. This method was applied to examine the relationship between ROS and aging, and find preventive agents again skin damage, and we found that ROS generation induced by UVA irradiation in the hairless rat (8-80 weeks old) skin increases age-dependently, and ascorbic acid-3-phosphate suppresses effectively oxidative stress in the skin of mice under UVA irradiation.
This method is useful not only for noninvasive investigation of the spatial distribution and quantitative determination of ROS in the skin of alive animals, but for in vivo evaluation of the protective ability of free radical scavengers and antioxidants.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] K.Yamato, I.Miyahara, A.Ichimura, K.Hirotsu, Y.Kojima, H.Sakurai: "Superoxide dismutase mimetic complex of Mn(II)/N, N-bis(1-pyridylmethyl)-(S)-histidine"Chem.Lett.. 295-296 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hitoshi Masaki, Yuri Okano, Hiromu Sakurai: "Generation of active oxygen species from advanced glycations end-products(AGEs) during ultraviolet light A(UVA) irradiation and a possible mechanism for cell damaging"Biochim.Biophys.Acta. 1428. 45-56 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mayoko Osada, Yuhko Ogura, Hiroyuki Yasui, Hiromu Sakurai: "Involvement of singlet oxygen in cytochrome P450-dependent substrate oxidations"Biochem.Biophys.Res.Commun.. 263. 392-379 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Yasui, Hiromu Sakurai: "Chemiluminescent detection and imaging of reactive oxygen species in live mouse skin exposed to UVA"Biochem.Biophys.Res.Commun.. 269. 131-136 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D.Gao, M.Kakuma, S.Oka, K.Sugino, H.Sakurai: "Reaction of β-alkannin(shikonin) with reactive oxygen species : detection of β-alkannin free radical"Bioorg.Med.Chem.. 8. 2561-2569 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dayuan Gao, Makoto Hiromura, Hiroyuki Yasui, Hiromu Sakurai: "Direct reaction between shikonin and thiols induces apoptosis in HL60 cells"Biol.Pharm.Bull.. 25. 827-832 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazuhiro Yamato, Ikuo Miyahara, Akio Ichimura, Ken Hirotsu, Yoshitane Kojima, and Hiromu Sakurai: "Superoxide dismutase mimetic complex of Mn(II)/N, N-bis(1-pyridylmethyl)-(S)-histidine."Chem. Lett.. 1999. 295-296 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hitoshi Masaki, Yuri Okano, and Hiromu Sakurai: "Generation of active oxygen species from advanced glycations end-products (AGEs) during ultraviolet light A (UVA) irradiation and a possible mechanism for cell damaging."Biochim. Biophys. Acta. 1428. 45-56 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mayoko Osada, Yuhko Ogura, Hiroyuki Yasui, and Hiromu Sakurai: "Involvement of singlet oxygen in cytochrome P450-dependent substrate oxidations."Biochem. Biophys. Res. Commun.. 263. 392-379 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Yasui and Hiromu Sakurai: "Chemiluminescent detection and imaging of reactive oxygen species in live mouse skin exposed to UVA."Biochem. Biophys. Res. Commun.. 269. 131-136 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dayuan Gao, Miyako Kakuma, Shigenori Oka, Keiji Sugino and Hiromu Sakurai: "Reaction of β-alkannin (shikonin) with reactive oxygen species : detection of β-alkannin free radical."Bioorg. Med. Chem.. 8. 2561-2569 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuri Okano, Hitoshi Masaki and Hiromu Sakurai: "Pentosidine in advanced glycation end-products (AGEs) during UVA irradiation generates active oxygen species and impairs human dermal fibroblasts."J. Dermatol. Science. 27. S11-S18 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shinya Kawanishi and Hiromu Sakurai: "Different anti-lipid peroxidative activity of melatonin."Naturwissenschaften. 89. 31-33 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Dayuan Gao, Makoto Hiromura, Hiroyuki Yasui, and Hiromu Sakurai: "Direct reaction between shikonin and thiols induces apoptosis in HL60 cells."Biol. Pharm. Bull.. 25. 827-832 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Yasui and Hiromu Sakurai: "Age-dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light."Exp. Dermatol.. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 桜井弘, 安井裕之: "紫外線により生じる皮膚中の活性酸素種の可視化"現代科学. 8. 45-51 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Yasui and H.Sakurai: "Chemiluminescent Detection and Imaging of Reactive Oxygen Species in Live Mouse Skin Exposed to UVA"Biochem.Biophys.Res.Commun.. 269. 131-136 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Yasui and H.Sakurai: "Chemiluminescent detection and imaging of reactive oxygen species in live hairless animal skin exposed to UVA"Book of abstracts.2000 International chemical congress of pacific basin societies. part1. 2.ANAL(167) (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y,Yamato: "Suparoxide dismutase mimetie complex of Mn(II)/N_1N-bis(1-pyridylmethyl)-(s)-histideine"Chem.Lett.. 1999. 295-296 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N.Nishino: "In vivo L-band ESR and guantitatire pharmacokinetic analysis of stable spin probes in rats and mice"Free.Rad.Res.. 31. 35-51 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Masaki: "Generation of active oxygen species from advanced glycations end-ptoducts(AGEs)during ultraviolet・・・・・・"Biochim.Ciophys.Acta.. 1428. 45-56 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Osada: "Involuamene of singlee oxygen in cytochrome P450-dependene substrate oxidations"Biochem.BioPhys.Res.Commun.. 263. 392-397 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Yasui: "Chemiluminescent detection and imaging of reactive oxygen species in live mouse skin exposed to UVA"Biochem.Biophys.Res.Commun.. 269. 131-136 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 桜井弘編著: "薬学のための分析化学"化学同人. 214 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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