| Project/Area Number |
11794002
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Nagaoka University of Technotogy |
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Principal Investigator |
OHASHI Akiyoshi Nagaoka University of Technology, Associate Professor, 工学部, 助教授 (70169035)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Nobuo Nagaoka National College of Technology, Associate Professor, 環境都市工学科, 助教授 (30193072)
HARADA Hideki Nagaoka University of Technology, Professor, 工学部, 教授 (70134971)
関口 勇地 長岡技術科学大学, 工学部, 助手 (20313570)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2001: ¥14,700,000 (Direct Cost: ¥14,700,000)
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| Keywords | Methane / Energy / Anaerobic digestion / Sewage treatment / Molecular technique / Microbiology / 分子生物学 / 下水処理 |
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| Research Abstract |
The aim of this study is to expand a technology of anaerobic digestion for treatment of organic solid waste such as activated sludge in sewage treatment system in order to enhance efficiency of methane gas recovery as energy. For making the efficiency bigger it was thought that biomass concentration in anaerobic digestion reactor should be increased. To investigate the biomass concentration effect a batch experiment, in which some materials supporting biofilm were installed, was conducted with activated sludge as substrate. The result indicated that biofilm having high biomass concentration could not promote biodegradation of solid waste. Tnen we examined amylase in an anaerobic digestion vial because hydrolysis enzyme was thought to play a crucial role. From the measurement of amylase we found a very important phenomenon that amylase was not released into bulk liquid as extra-cellar enzyme but it would be linked to cell membrane. This suggests that even though biomass concentration is high, hydrolysis of solid waste does not become proceed without concents of the solid waste and microorganisms. Therefore ozone treatment of solid waste was applied before fermented to improve the efficiency of methane recovery. The result revealed that the ozone treatment was effective for the hydrolysis of the solid waste and would be economically feasible. It is important for having a good idea of anaerobic digestion enhancement to understand satisfactory microbial community retained in the reactor. To analyze the microbial community molecular techniques were used. We also isolated several key novel anaerobic syntrophic bacteria and investigated these characteristics. From these results the structure and function of the microbial consortia were understood approximately half.
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