| Project/Area Number |
11794003
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Kobe University |
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Principal Investigator |
NAKAMURA C Kobe University, Fac. Agriculture, Professor, 農学部, 教授 (10144601)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIJIMA O. Kobe University, Fac. Agriculture, Professor, 農学部, 教授 (30031222)
TAKUMI S. Kobe University, Fac. Agriculture, Assistant Professor, 農学部, 助手 (50249166)
MORI N. Kobe University, Fac. Agriculture, Associate Professor, 農学部, 助教授 (60230075)
ASAKURA W. Kanagawa University, Fac. Engineering, Lecturer, 工学部, 講師 (80301589)
吉田 晋弥 兵庫県農業技術センター, 生物工学部, 主任研究員
YOSHIDA S. Hyogo Agricultural Research Institute, Senior Researcher
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2001: ¥13,500,000 (Direct Cost: ¥13,500,000)
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| Keywords | Sake-brewing rice / Genetic resources / Molecular markers / Cataloging / Phylogenetic analysis / QTL analysis / Mutator transposon / トランスポゾン / OsMu / 多型解析 / AFLP / SSRP / 心白発現 / 低温耐性・感受性 / OsMuトランスポゾン / Tos / OsMuDR |
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| Research Abstract |
1) Cataloging of sake-brewing rice genetic resources by molecular markers. Sake-brewing rice genetic resources were cataloged based of growth and grain characteristics and AFLP and SSR markers in 253 cultivars (Submitted to Theoretical and Applied Genetics).
2) Genetic and QTL analyzes of the white-core rate (Shinpaku rate) and other sake-brewing characteristics. At least five genes were identified as regulators of the white-core rate using a near-isogenic lines (Breeding Science, in prep.). Using a doubled haploid population derived from a Yamadanishiki x Reihou cross, QTLs related to growth and grain characters of sake-brewing rice including Shinpaku rate were identified and mapped on nee chromosomes (Breeding Science, in press). A similar QTL analysis was conducted to identify QTLs for sake-brewing characterstics using F2 and F3 populations of a Hokuriku42 x Hyogokitanishiki cross.
3) Isolation of novel Tos members and selection of mutant lines/ Using primers for the reverse transcriptase gene of Tos, five novel Tos members of retrotransposons were identified from cultured cells of Hyogoyumenishiki. Through anther culture of gammairradiated young inflorescences of Yamadanishikl several mutant lines were obtained.
4) Isolation and characterization of rice orthologs of maize Mu transposon. Two rice orthologs (OsMuDR) of maize Mu trransposons were isolated by PCR amplification of the previously isolated partial cDNA sequence homologous to the maize MuDR. One clone (OsMu4-1) was an intact sequence with TIRs and TDSs, while another (OsMu10-1) was a deleted version that was likely caused by the interrupted-gap-repair mechanism. OsMu4-1 was proven to have transposed in the past and is still transcriptionally active but a pseudogene. Alternatively spliced transcripts were identified from OsMu4-1. A wide distribution of OsMu elements was shown by Southern blot analysis of wild relatives of rice and data mining of the rice genome. (Molecular Genetics and Genomics, in press).
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