| Project/Area Number |
11794013
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
生物資源科学
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| Research Institution | Nagasaki University |
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Principal Investigator |
KODAMA Seiji Nagasaki University, School of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (00195744)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Hiroshi Nagasaki University, School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (00145674)
KOBAYASHI Nobuyuki Nagasaki University, School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (30150329)
WATANABE Masami Nagasaki University, School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (20111768)
OKAICHI Kumio Nagasaki University, School of Medicine, Associate Professor, 医学部, 助教授 (80124874)
ODA Tatsuya Nagasaki University, Faculty of Fisheries, Professor, 水産学部, 教授 (60145307)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2001: ¥13,000,000 (Direct Cost: ¥13,000,000)
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| Keywords | marine microorganism library / inhibition of melanogenesis / inhibition of tumor cell growth / red tide phytoplankton / active oxygens / mannuronate / inhibition of cell motility / endocrine desruptors / 細胞増殖阻害 / ゲノムDNAライブラリー / 微生物ライブラリー / グルロン酸 / アルギン酸 |
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| Research Abstract |
We constructed a marine microorganism library to find the bacteria that produce materials useful for developing new foods or drugs. This microorganism library provides us the following new results. First, we found two strains of marine bacteria showing the ability to inhibit melanogenesis that is comparable to that of 0. 25 mM ascorbic acid by screening 548 strains of marine bacteria. The bacteria-secreted inhibitors are heat-resistant and show no reductive activity. Then, we also found 24 strains of marine bacteria showing the ability to inhibit the growth of colon cancer cells by screening 2,480 strains of marine bacteria. In addition, we found that mannuronate polymer, one of components of alginate, suppressed the motility of glioblastoma cells having wild-type p53 gene after X-irradiation. Furthermore, we found that galacturonic acid stimulated Chattonella marina and Heterosigna akashiwo to generate increased amounts of O_2-, suggesting that the binding of galacturonic acid to specific sites on the flagellate cell surface may induce the increase of O_2- production. Finally, we established in vitro and in vivo screening systems that were hypersensitive to detect endocrine desruptors in the marine resources.
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