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Development of Biosensor-type-DNA Chip for the Diagnosis of Cancer

Research Project

Project/Area Number 11794017
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionKanazawa University

Principal Investigator

KANEKO Shuichi  Kanazawa University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (60185923)

Co-Investigator(Kenkyū-buntansha) TAMIYA Eiichi  Japan Advanced Institute of Science and Technology, School of Materials Science, Professor, 教授 (60179893)
NAKAMOTO Yasurari  Kanazawa University, Graduate School of Medicine, Assistant Professor, 医学部・附属病院, 助手 (40293352)
HONDA Masao  Kanazawa University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (00272980)
ARAKAWA Hisao  Hokutokagaku Co., Development, Manager, 室長(研究職)
KAWAKAMI Bunsei  Toyobo Co., Gene Analysis, Chief, 主席部員(研究職)
佐々木 基  澁谷工業株式会社, 主事
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2001: ¥15,000,000 (Direct Cost: ¥15,000,000)
KeywordsBiosensor / Cancer / DNA chip / SAGE / Molecular Diagnosis / 遺伝子
Research Abstract

Development of a biosensor-type-DNA chip for the diagnosis of cancer is an objective of this research. To attain this objective, two lines of research projects were designed.
For the first line of the project, we systematically examined expression profiles in the liver with various diseases including hepatocellular carcinoma. Over one hundred clinical materials were analyzed with home-made DNA chips, and the expression profile in each disease was demonstrated. This study revealed that a DNA chip analysis gives new information that cannot be obtained by routine biochemical and pathological examinations, and made the DNA chip be a new diagnostic technique, or a new tumor marker. Using serial analysis of gene expression (SAGE), over 300,000 expressed tags in the liver were obtained and deposited into databases. A DNA chip, which predicts the efficacy of drugs against chronic hepatitis C, was put on the market.
Another line of the project is to make a biosensor-type-DNA chip, which can be used in routine clinical settings on site. Using microelectrodes on a chip, target nucleic acids can be measured in liquid (applied for a patent in 2001). A small-size thermal cycler for the introduction of measurement on microelectrodes was developed (applied for a patent in 2000). A DNA spotter on a microelectrode was also developed (pending for a patent). Using these successive developments, a biosensor-type-DNA chip, which is small-size, less expensive compared with former laser-detection-type DNA chips, and faster for the clinical diagnosis, was made.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] Eishiro Mizukoshi: "Serum level of soluble interferon alpha/beta receptor as an inhibitory factor of IFN in the patients with chronic hepatitis C"Hepatology. 30. 1325-1331 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akito Sakai: "Quasispecies of hepatitis C virus in serum and in three different parts of the liver of patients with chronic hepatitis"Hepatology. 30. 556-561 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunari Nakamoto: "Inhibition of peripheral blood lymphocyte apoptosis by soluble Fas ligand in patients with hepatocellular carcinoma"Oncology Report. 6. 733-739 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eishiro Mizukoshi: "Up-regulation of Type I interferon receptor by interferon-gamma"Journal of Interferon and Cytokine Research. 19. 1019-1023 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takayoshi Koura: "Investigation of albumin-synthesizing ability in rat cirrhotic liver-directed hepatocytes using primary hepatocyte culture"Journal of Hepatology. 31. 293-299 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hideki Ohono: "Human hepatitis B virus X protein augments the DNA binding of NF-IL6 through its basic-leucine zipper domain"Journal of Medical Virology. 58. 11-18 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Rieko Saeki: "Intratumoral genomic heterogeneity in human hepatocellular carcinoma detected by restriction landmark genomic scanning"Journal of Hepatology. 33. 99-105 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taro Yamashita: "Comprehensive gene expression profile of a normal human liver"Biochemical and Biophysical Research Communications. 269. 110-116 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Honda: "Cell cycle regulation of hepatitis C virus IRES-directed translation"Gastroenterology. 118. 152-162 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aki Takeuchi: "Interferon-alpha modulates resistance to cisplatin in three human hepatoma cell lines"Journal of Gastroenterology. 34. 351-358 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taro Yamashita: "Serial Analysis of Gene Expression in Chronic Hepatitis C and Hepatocellular Carcinoma"Biochemical and Biophysical Research Communications. 282. 647-654 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yukihiro Shirota: "Identification of differentially expressed genes in hepatocellular carcinoma with cDNA microarrays"Hepatology. 33・4. 832-840 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Honda: "Differential Gene Expression Between Chronic Hepatitis B and C Hepatic Lesion"Gastroenterology. 120・4. 955-966 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi F.Kawai: "Alpha-fetoprotein-producing hepatoma cell lines share common cDNA microarray analysis"Hepatology. 33・3. 676-691 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaaki Kobayashi: "Electrochemical Gene Dection Using Microelectrode Array on a DNA Chip"Electrochemistry. 69. 1013-1016 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaaki Kobayashi: "Electrochemical Gene Detectin Using Microelectrode Array and PCR"New Technology Japan. 29. 11-17 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 民谷栄一: "バイオセンサーチップ"BME. 15. 8-16 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaaki Kobayashi: "Electrochemical Gene Detedtion with PCR Chip"Transducers 01' Eurosesors XV. 5 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eishiro Mizukoshi: "Serum level of soluble interferon alpha/beta receptor as an inhibitory factor of IFN in the patients with chronic hepatitis C"Hepatology. 30. 1325-1331 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akito Sakai: "Quasispecies of hepatitis C virus in serum and in three different parts of the liver of patients with chronic hepatitis"Hepatology. 30. 556-561 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunari Nakamoto: "Inhibition of peripheral blood lymphocyte apoptosis by soluble Fas ligand in patients with hepatocellular carcinoma"Oncology Report. 6. 733-739 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eishiro Mizukoshi: "Up-regulation of Type I interferon receptor by interferon-gamma"Journal of Interferon and Cytokine Research. 19. 1019-1023 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takayoshi Koura: "Investigation of albumin-synthesizing ability in rat cirrhotic liver-directed hepatocytes using primary hepatocyte culture"Journal of Hepatology. 31. 293-299 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hideki Ohono: "Human hepatitis B virus X protein augments the DNA binding of NF-IL6 through its basic-leucine zipper domain"Journal of Medical Virology. 58. 11-18 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Rieko Saeki: "Intratumoral genomic heterogeneity in human hepatocellular carcinoma detected by restriction landmark genomic scanning"Journal of Hepatology. 33. 99-105 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Honda: "Cell cycle regulation of hepatitis C virus IRES-directed translation"Gastroenterology. 118. 152-162 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taro Yamashita: "Comprehensive gene expression profile of a normal human liver"Biochemical and Biophysical Research Communications. 269. 110-116 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aki Takeuchi: "Interferon-alpha modulates resistance to cisplatin in three human hepatoma cell lines"Journal of Gastroenterology. 34. 351-358 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taro Yamashita: "Serial Analysis of Gene Expression in Chronic Hepatitis C and Hepatocellular Carcinoma"Biochemical and Biophysical Research Communications. 282. 647-654 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yukihiro Shirota: "Identification of differentially expressed genes in hepatocellular carcinoma with cDNA microarrays"Hepatology. 33-4. 832-840 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Honda: "Differential Gene Expression Between Chronic Hepatitis B and C Hepatic Lesion"Gastroenterology. 120-4. 955-966 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroshi F. Kawai: "Alpha-fetoprotein-producing hepatoma cell lines share common expression profiles of genes in various categories demonstrated by cDNA microarray analysis"Hepatology. 33-3. 676-691 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaaki Kobayashi: "Electrochemical Gene Dection Using Microelectrode Array on a DNA Chip"Electrochemistry. 69. 1013-1016 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaaki Kobayashi: "Electrochemical Gene Detectin Using Microelectrode Array and PCR"New Technology Japan. 29. 11-17 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamiya Eiichi: "Biosensor-chip"BME. 15. 8-16 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaaki Kobayashi: "Electrochemical Gene Detedtion with PCR Chip"Transducers 01' Eurosesors XV. 5 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Taro Yamashita: "Serial Analysis of Gene Expression in Chronic Hepatitis C and Hepatocellular Carcinoma"Biochemical and Biophysical Research Communications. 282. 647-654 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yukihiro Shirota: "Identification of differentially expressed genes in hepatocellular carcinoma with cDNA microarrays"Hepatology. 33・4. 832-840 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masao Honda: "Differential Gene Expression Between Chronic Hepatitis B and C Hepatic Lesion"Gastroenterology. 120・4. 955-966 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroshi F.Kawai: "Alpha-fetoprotein-producing hepatoma cell lines share common expression profiles of genes in various categories demonstrated by cDNA microarray analysis"Hepatology. 33・3. 676-691 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masao Honda, et al.: "cDNA microarray analysis reveals differential gene expression between chronic hepatitis B and C hepatic lesions."Gastroenterology. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Yukihiro Shirota, et al.: "Identification of differentially expressed genes in hepatocellular carcinoma with cDNA microarrays."Hepatology. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hiroshi Kawai, et al.: "Alpha-fetoprotein producing hepatoma cell lines shares common gene expression profile covering various gene categories in cDNA microarray analysis."Hepatology. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Rieko Saeki: "Intratumoral genomic heterogeneity in human hepatocellular carcinoma detected by restriction landmark genomic scanning"Journal of Hepatology. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Taro Yamashita: "Comprehensive gene expression profile of a normal human liver"Biochemical and Biophysical Reserch Communications. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Masao Honda: "Cell cycle regulation of hepatitis C virus IRES-directed translation"Gastroenterology. 118. 152-162 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Aki Takeuchi: "Interferon-alpha modulates resistance to cisplatin in three human hepatoma cell lines"Journal of Gastroenterology. 34. 351-358 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuro Koike: "Survey of hepatitis B virus co-infection in hepatitis C virus infected patients suffering from chronic hepatitis and hepatocellular carcinoma in Japan"Japanese Journal of Cancer Research. 90. 1270-1272 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Miyoko Natsume: "Attenuated liver fibrosis and depressed serum albumin levels in carbon tetrachloride-treated IL-6-deficient mice"Journal of Leukocyte Biology. 66. 601-608 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Eishiro Mizukoshi: "Serum level of soluble interferon alpha/beta receptor as an inhibitory factor of IFN in the patients with chronic hepatitis C"Hepatology. 30. 1325-1331 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akito Sakai: "Quasispecies of hepatitis C virus in serum and in three different parts of the liver of patients with chronic hepatitis"Hepatology. 30. 556-561 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasunari Nakamoto: "Inhibition of peripheral blood lymphocyte apoptosis by soluble Fas ligand in patients with hepatocellular carcinoma"Oncology Report. 6. 733-739 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Eishiro Mizukoshi: "Up-regulation of Type I interferon receptor by interferon-gamma"Journal of Interferon and ytokine Research. 19. 1019-1023 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takayoshi Koura: "Investigation of albumin-synthesizing ability in rat cirrhotic liver-directed hepatocytes using primary hepatocyte culture"Journal of Hepatology. 31. 293-299 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hideki Ohono: "Human hepatitis B virus X protein augments the DNA binding of NFIL6 through its basic-leucine zipper domain"Journal of Medical Virology. 58. 11-18 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shuichi Kaneko: "In Nagy Habib (eds) : Methods in Molecular Medicine""Gene therapy vectors harvoring AFP regulatory sequences : preparation of an adenoviral vector (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Shuichi Kaneko: "In Sudhir Srivastava (eds) : "Molecular Pathology of Early Cancer""Liver Cancer. 9 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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