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Isolation and Characterization of Biological Active Materials from Fish Skin Waste

Research Project

Project/Area Number 11794018
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionTottori University

Principal Investigator

SATO Kenzo  Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (40113196)

Co-Investigator(Kenkyū-buntansha) AYAKI Yoshikazu  National College of Technology, Professor, 物質工学, 教授 (90032002)
MORIYAMA Masatsugu  Tottori University, Faculty of Medicine, Associate. Professor, 医学部, 助教授 (90239707)
HASHIMOTO Eikich  Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (20116239)
KASHIWAGI Kouji  Daimatsu Co, Ltd. Sinner Researcher, 研究開発部, 技術員
NOGUCHI Makoto  Industrial Research Institute of Tottori Prefecture, Sinner Researcher, 応用技術部, 研究員
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 2001: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsfish skin / collagen / liver function / Biological activity / HGF / extracellular matrix components / screening system / スクリーニングシステム / 骨形成 / オステオカルシン / レポーターアッセイ / レポーター遺伝子
Research Abstract

In the marine food industry, fish skin is discarded as waste like bone and entrails, and these waste materials make-up more than a half of the whole body weight, desired for utilization in some other products. The skin of fish, which is composed of about 10% of fish waste, can be mechanically removed and readily recovered in food processing. The skin of a halibut Reinhararditus hippoglossoides, which is a main marine-food processing product in Tottori Prefecture Japan, contains 34% protein and related materials, 14% oil, and 52% water. The utilization of this fish skin is expected to contribute the development of local industry. Incidentally, liver diseases, such as hepatitis, liver cirrhosis and hepatoma were reported to show a tendency of rapid increase recently within Japanese people in the working generation of middle ages. It has also become an important subject to be urgently developed for therapeutic or ameliorative medication. At first in this project, the extraction and puriflcation process of collagen and extracellular matrix components were established using fish skin. As the application of fish collagen, we have succeeded the three dimensional culture of primary hepatocytes, and revealed that liver function was well maintained in this culture. Furthermore, we showed that proteoglycan crude extracts from the fish skin has significant inducibility of HGF in culture cell. Especially, of the separated glycosaminoglycans, dermatan sulfate which was the main component of glycosaminoglycans in the fish skin, showed the strongest activity in HGF induction. As the use of cattle collagen is restricted for the infection of the prion that is the cause of BSE and the Creutzfeldt-Jakob disease, the importance of fish collagen should be re-evaluated.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Tanaka, F, et al.: "Identification of differentially expressed genes in rat hepatoma cell lines using subtraction and microarray"J.Biochem.. 131. 39-44 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishiguro, N, et al: "CARP, a putative genetic marker for cardiac hypertrophy, and its new homologue, Arpp, are differentially expressed in heart, skeletal muscle, and rhabdomyosarcomas"Am.J.Pathol.. 160. 1767-1778 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsukamoto, Y., et al: "Arpp, a new homolog of carp, is preferentially expressed in type 1 skeletal muscle fibers and is markedly induced by denervation"Lab Invest.. 82. 645-655 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Goto, N., et al: "A serpin with Mr 43,000 is a binding protein of Mr 25,000 protein, a substrate for protein ser/thr kinase detected in Xenopus laevis oocyte"J.Biochem. 129. 229-236 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funami J, et al: "Disappearance of Mr 25,000 protein, a new protein kinase substrate, in parallel with a kind of serpin during embryogenesis"J.Biochem.Mol.Biol.Biophys.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeuchi, et al: "Multiple elements for negative regulation of the rat catalase gene expression in dedifferentiated hepatoma cells"J.Biochem.. 128. 1025-1031 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato, K.: "Experimental protocoles for reactive oxygen and nitrogene species (ed.Taniguchi & Gutteridge)"Oxford Univ.Press. 357 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, T., Akiyoshi, H., Saito, I. and Sato, K.: "Adenovirus-mediated gene expression in the fibroblast-like cells of cirrhotic rat livers."J. Hepatol.. 30. 101-106 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Goto, A., Shomori, K., Ohkumo, T., Tanaka, F., Sato, K. and Ito, H.: "Hyperthermia-induced apoptosis occurs both in a p53 gene-dependent and independent manner in three human gastric carcinoma cell lines."Oncology Rep.. 6. 335-339 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatebe, S., Matsuura, T., Endo, K, Teramachi, K., Nakamura, T., Sato, K. and Ito, H.: "Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells."Int. J. Oncology. 15. 229-235 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakamura, T., Akiyoshi, H., Shiota, G., Isono, M., Nakamura, K., Moriyama, M. and Sato, K,: "Hepatoprotective action of adenovirus-transferred HNF-3γ gene in acute liver injury caused by CC14."FEBS Letters. 459. 1-4 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang, H., Gao, X., Fukumoto, S., Tademoto, S., Sato, K., and Hirai, K.: "Differential expression and regulation of chemokines JE, KC, and IP-10 gene in primary cultured murine hepatocytes."J. Cell. Physiol.. 181. 361-370 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saito, K., Nakamura, T., Komoda, H., Hori, N., Adachi, K., Ito, K., and Sato. K.: "Isolation and characterization of the rat hepatocyte nuclear factor-3 gamma (HNF-3 gamma) gene."Res Commun. Biochem Cell Mol. Biol.. 3. 157-169 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiota, G., Kunisada, T., m Oyama, K., Udagawa, A., Nomi, T., Tanaka, K., Tsutsumi, A., isono, M., Nakamura, T., Hamada, H., Sakatani, T., Sell, S., Sato, K., Ito, H., and Kawasaki, H.: "In vivo transfer of hepatocyto growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats."FEBS Letters. 470. 325-330 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takeuchi, T., Nakamura, S., Kayasuga, A., Isa, S., and Sato, K.: "Multiple elements for negative regulation of the rat catalase gene expression in dedifferentiated hepatoma cells."J Biochem.. 128. 1025-1031 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nomi T, Shiota G, Isono M, Sato K, and Kawasaki H.: "Adenovirus-mediated hepatocyte growth factor gene transfer prevents lethal liver failure in rats."Biochem Biophys Res Commun. 278. 338-343 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka, F., Hori, N., and Sato, K.: "Identification of differentially expressed genes in rat hepatoma cell lines using subtraction and microarray."J. Biochem.. 131. 39-44 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishiguro, N., Baba, T., Ishida, T., Takeuchi, K., Osaki, M., Araki, N., Okada, E., Takahashi, S., Saito, M., Watanabe, M., Nakada, C., Tsukamoto, Y., Sato, K., Ito, K., Fukayama, M., Mori, S., Ito, H. and Moriyama, M: "CARP, a putative genetic marker for cardiac hypertrophy, and its new homologue, ARPP, are differentially expressed in heart, skeletal muscle and rhabdomyosarcoma."Am J Pathol.. 160(5). 1767-1778 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsukamoto Y, Senda T, Nakano T, Nakada C, Hida T, Ishiguro N, Kondo G, Baba T, Sato K, Osaki M, Mori S, Ito H, Moriyama M. Arpp: "a new homolog of carp, is preferentially expressed in type 1 skeletal muscle fibers and is markedly induced by denervation."Lab Invest.. 82(5). 645-655 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasokawa M., Goto N. and Hashimoto E.: "Inhibition of mitogen-activated protein kinase by (-)-epigallocatechin gallate in vitro."J. Biochem. Mol. Biol. Biophys. 3. 177-181 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasokawa M., Sugimoto I., Fukuma M., Goto N., Kobayashi N., Yasuda H., Ogura K., Iwashita J., Nagata K., Hiji Y. and Hashimoto E.: "(-)- epigallocatechin gallate inhibits Mos activation-mediated Xenopus oocyte maturation induced by progesterone"FEBS Lett.. 463. 317-320 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Goto N., Muto S., Sugimoto l., Ikawa-Kitayama K., Igarashi K., Ito S. and Hashimoto E: "A serpin with Mr 43,000 is a binding protein of Mr 25,000 protein, a substrate for protein Ser/Thr kinase detected in Xenopus laevis oocytes"J. Biochem.. 129. 229-236 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funami J., Miyoshi N., Sugimoto I. and Hashimoto E: "Disappearance of Mr 25,000 protein, a new protein kinase substrate, in parallel with a kind of serpin during embryogenesis"J. Biochem. Mol. Biol. Biophys. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kohara H., Funami J. and Hashimoto E.: "Expression of Xenopus SET mRNAs during development"Res. Commun. Biochem. Cell Mol. Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Moriyama, M.: "Identification of a novel human ankyrin-repeated protein homologous to CARP"Biochem. Biophys. Res. Comm.. 285. 715-723 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamagata, K.: "High expression of Apolipoprotein E mRNA in the brains with sporadic Alzheimer's disease"Dement. Geriatr. Cogn. Disord.. 12. 57-62 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kaneita, Y: "Detection of reciprocal fusion 5'-BCL6/partner-3' transcripts in lymphomas exhibiting reciprocal BCL6 translocations"British J. Haematology. 113. 803-806 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanaka, F: "Identification of differentially expressed genes in rat hepatoma cell lines using subtraction and microarray"J. Biochem.. 131. 39-44 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyazaki, A.: "Genomic organization of the human Arpp gene"Yonago Acta Med.. 45. 1-8 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishiguro, N: "CARP, a putative genetic marker for cardiac hypertrophy, and its new homologue, ARPP, are differentially expressed in heart, skeletal muscle and rhabdomyosarcoma"Am. J. Pathol.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takauchi,T.: "Multiple elements for negative regulation of the rat catalase gene expression characterized in dedifferentiated hepatoma cell."J.Biochem.. 128. 1025-1031 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ohsawa,T.: "Enhancement of adenovirus-mediated gene transfer into dermal fibroblasts in vitro and in vivo by polyethylene glycol 6000"J.Dermatol.. 27. 244-251 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shiota,G.: "In vivo transfer of hepatocyto growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats."FEBS Letters. 470. 325-330 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Endo,T.: "A pivotal role of Zn-binding residues in the function of the copper chaperone for SOD1"Biochem.Biophys.Res.Commun.. 276. 999-1004 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nomi,T.: "Adenovirus-mediated hepatocyte growth factor gene transfer prevents lethal liver failure in rats"Biochem.Biophys.Res.Commun.. 278. 338-343 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Goto,N.: "A serpin with Mr43,000 is a binding protein of Mr25,000 protein,a substrate for protein Ser/Thr kinase detected in Xenopus laevis oocytes"J.Biochem.. 129. 229-236 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sato,K.(共著): "Experimental Protocols for Reactive Oxygen and Nitrogen Species"Oxford University Press.UK. 357 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nakamura: "Hepatoprotective action of adenovirus-transferred HNF-3γ gene in acute liver injury caused by CCC_4"FEBS Letters. 459・1. 1-4 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Nakamura: "Adenovirus-mediated gene expression in the septal cells of cirrhotic rat livers"J.Hepatol.. 30・1. 101-106 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Wang,H.: "Differential expression and regulation of chemokinases JE,KC,and IP-10 gene in primary cultured murine hepotocytes"J.Cell.Physiol.. 181. 361-370 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasokawa,M.: "(-)-Epigallo catechin gallate inhibits Mos activation-mediated Xenopus oocyte maturation induced by progesterone"FEBS Letters. 463. 317-320 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasokawa,M.: "Inhibition of mitogew-activated protein kinase by (-)-Epigallocatechin gallate in vitro"J.Biochem.Mol.Biol.Biophys.. 3. 177-181 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 野口 誠: "高圧処理による醤油中のZ.rouxiiの殺菌"日本醸造協会誌. 95・1. 46-52 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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