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Evaluation of toxicity of environmental chemicals on the basis of their estrogenic activity and DNA-damaging ability and regional risk assessment

Research Project

Project/Area Number 11794019
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field Public health/Health science
Research InstitutionMie University

Principal Investigator

KAWANISHI Shosuke  Mie University, School of Medicine, Professor, 医学部, 教授 (10025637)

Co-Investigator(Kenkyū-buntansha) OIKAWA Shinji  Mie University, School of Medicine, Lecturer, 医学部, 講師 (10277006)
MURATA Mariko  Mie University, School of Medicine, Lecturer, 医学部, 講師 (10171141)
NAKASHIMA Kunio  Mie University, School of Medicine, Professor, 医学部, 教授 (40022800)
HASHIZUME Kiyoshi  Mie Prefecural Science and Technology Promotion Center, Public Health and Environment Research Division, General Researcher, 保健環境研究所衛生研究グループ, 総括研究員
HIRAKU Yusuke  Mie University, School of Medicine, Assistant Professor, 医学部, 助手 (30324510)
大熊 和行  三重県科学技術振興センター, 保健環境研究所, 主幹研究員兼衛生科学グルブリダ
山下 成人  三重大学, 医学部, 助手 (40263024)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 2001: ¥12,000,000 (Direct Cost: ¥12,000,000)
KeywordsEnvironmental chemicals / Endocrine disruption / Reproductive toxicity / Carcinogenicity / Genetic damage / Estrogen / Phytoestrogen / Trinitrotoluene / 発がん / ホルモン作用 / 農薬 / ヒト遺伝子損傷性 / 精子毒性 / トルエン / エストロゲン活性 / ピレスロイド系農薬 / ニトロピレン / 残留農薬一斉微量分析 / 遺伝子損傷性 / 毒性評価 / 地域 / リスク評価 / エストロゲン依存増殖性乳腺腫瘍細胞(MCF-7)
Research Abstract

There has been an alarming concern among general public regarding the reproductive and health hazards of the endocrine disrupting environmental chemicals. Reproductive toxicants are considered to exert their toxicity through not only endocrine disruption but also direct damage to reproductive organs. In fact, a number of carcinogens exhibit reproductive toxicity. Thus, such chemicals may show reproductive toxicity through DNA damage. In the present study, we investigated the role of oxidative DNA damage induced by various environmental chemicals in relation to their carcinogenicity and reproductive toxicity. (1) It is important to clarify the mechanism of carcinogenesis induced by estrogens. Estradiol enhanced proliferation of estrogen-dependent MCF-7 cells at much lower concentrations than its metabolites, catechol estrogens, but did not cause DNA damage. On the other hand, catechol estrogens caused DNA damage at extremely low concentrations. Therefore, catechol estrogens appear to pl … More ay a role in tumor initiation through oxidative DNA damage, whereas estrogens themselves induce tumor promotion by enhancing cell proliferation in estrogen-induced carcinogenesis (Int. J. Cancer, 2001). We found that daidzein, contained in soy beans, and its metabolite, 7 ,3',4'-trihydroxyiosoflavone, may induce carcinogenesis in a similar manner. (2) 2,4,6-Trinitrotoluene (TNT), which is used to produce explosives, has been reported to cause reproductive toxicity and cancer. TNT administration to rats induced a dramatic decrease in the sperm number in the testis and an increase in 8-oxodG formation in the testis and epididymis. A TNT metabolite, induced oxidative damage to ^<32>P-labeled DNA fragments, while TNT itself did not. These findings suggest that TNT induces reproductive toxicity through oxidative DNA damage mediated by its metabolite (Free Radic. Res., in press). We also found that toluene may cause reproductive toxicity in a similar manner (BBRC, 1999). In conclusion, these findings suggest that environmental chemicals may exhibit reproductive toxicity and carcinogenicity through oxidative DNA damage. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (124 results)

All Other

All Publications (124 results)

  • [Publications] S.Homma-Takeda: "2,4,6-Trinitrotoluene-induced Reproductive Toxicity via Oxidative DNA Damage by its Metabolite"Free Radic Res. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Kawanishi: "The role of metals in site-specific DNA damage with reference to carcinogenesis"Free Radic.Biol.Med.. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Murata: "Oxidation of 5'-site guanine at GG and GGG sequences induced by a metabolite of carcinogenic heterocyclic amine PhIP in the presence of Cu(II) and NADH"Carcinogenesis. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ohnishi: "DNA damage induced by hypochlorite and hypobromite with reference to inflammation-associated carcinogenesis"Cancer Lett.. 178. 37-42 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Hiraku: "Determination of intracellular glutathione and thiols by high performance liquid chromatography with a gold electrode at the femtomole level : comparison with a spectroscopic assay"Biochim Biophys Acta.. 1570. 47-52 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Hirakawa: "Catechol and Hydroquinone Have Different Redox Properties Responsible for Their Differential DNA-damaging Ability"Chem.Res.Toxicol. 15. 76-82 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ohnishi: "Double Base Lesions of DNA by a Metabolite of Carcinogenic Benzo[a]pyrene"Biochem.Biophys.Res.Commun.. 290. 778-782 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Kawanishi: "Mechanism of Guanine-specific DNA Damage by Oxidative Stress and Its Role in Carcinogenesis and Aging"Mutation Res.(Reviews in Mutation Research). 488. 65-76 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Kawanishi: "Oxidative DNA damage in cultured cells and rat lungs by carcinogenic nickel compounds"Free.Radic.Biol.Med.. 31. 108-116 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Hishita: "Caspase-3 Activation by Lysosomal Enzymes in Cytochrome c-independent Apoptosis in a Myelodysplastic Syndrome (MDS)-derived Cell Line P39"Cancer Res.. 61. 2878-2884 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Oikawa: "Site-specific DNA Damage at GGG Sequence by UVA Involves Acceleration of Telomere Shortening"Biochemistry. 40. 4763-4768 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Midorikawa: "Superoxide dismutases enhance H_2O_2-induced DNA damage and alter its site specificity"FEBS Lett.. 495. 187-190 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Murata: "Acrylonitril Enhances H_2O_2-Mediated DNA Damage via Nitrogen-Centered Radical Formation"Chem.Res.Toxicol.. 14. 1421-1427 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Oikawa: "Site Specificity and Mechanism of Oxidative DNA Damage Induced by Carcinogenic Catechol"Carcinogenesis. 22. 1239-1245 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Murata: "Mechanism of Oxidative DNA Damage Induced by Carcinogenic 4-Aminobiphenyl"Free Radic.Biol Med.. 30. 765-773 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Murata: "Requirement of Glutathione and Cysteine in Guanine-specific Oxidation of DNA by Carcinogenic Potassium Bromate"Chem.Res.Toxicol.. 14. 678-685 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Sakano: "Hydroxyurea Induces Site-specific DNA Damage via Formation of Hydrogen Peroxide and Nitric Oxide"Jpn.J.Cancer Res.. 92. 1166-1174 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Ohkuma: "Sequence-specific DNA damage induced by carcinogenic danthron and anthraquinone in the presence of Cu(II), cytochrome P450 reductase and NADPH"Free.Radic.Res.. 34. 595-604 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ohnishi: "Oxidative DNA damage induced by an N-hydroxy metabolite of carcinogenic 4-dimethylaminoazobenzene"Jpn.J.Cancer Res. 92. 23-29 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Sakano: "Mechanism of Metal-mediated DNA Damage Induced by Metabolites of Carcinogenic 2-Nitropropane"Mutat.Res.. 479. 101-111 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ohnishi: "Oxidative DNA Damage by an N-Hydroxy Metabolite of the Mutagenic Compound Formed from Norharman and Aniline"Mutat.Res.. 494. 63-72 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Midorikawa: "Protective Effect of Phytic Acid on Oxidative Damage with Reference to Cancer Chemoprevention"Biochem.Biophys.Res.Commun. 288. 552-557 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Ohkuma: "Oxidative DNA Damage Induced by a Metabolite of Carcinogenic o-Anisidine : Enhancement of DNA Damage and Alteration in its Sequence-Specificity by Superoxide Dismutase"Arch Biochem.Biophys.. 389. 49-56 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ohnishi: "Oxidative DNA Damage by a Metabolite of Carcinogenic 1-Nitropyrene"Biochem.Biophys.Res.Commun.. 280. 48-52 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Hiraku: "Amplification of Bleomycin-induced DNA Cleavage at Cytosine Residues 3' to GGG Sequences by Pyrrole Triamide"Biochem.Pharmacol.. 61. 351-356 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Hiraku: "Catechol Estrogens Induce Oxidative DNA Damage and Estradiol Enhances Cell Proliferation"Int.J.Cancer. 92. 333-337 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 及川伸二: "環境因子による酸化的DNA損傷とがん、老化"Environ.Mut.Res.Commun.. 23. 207-213 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Murata: "Oxidative DNA Damage by Vitamin A and its Derivative via Superoxide Generation"J.Biol.Chem.. 275. 2003-2008 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Kawanishi: "Amplification of Pepleomycin-mediated DNA Cleavage and Apoptosis by Unfused Aromatic Cations"Biochemistry. 39. 13210-13215 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Hiraku: "Distinct Mechanisms of Guanine-specific DNA Photodamage Induced by Nalidixic Acid and Fluoroquinolone Antibacterials"Arch.Biochem.Biophys.. 382. 211-218 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ohnishi: "Copper-dependent DNA Damage Iinduced by Hydrazobenzene, an Azobenzene Metabolite"Free Radic.Res.. 32. 469-478 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Midorikawa: "DNA Damage by Dimethylformamide : Role of Hydrogen Peroxide Generated During Degradation"Chem.Res.Toxicol.. 13. 309-315 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Murata: "Mechanism of Oxidative DNA Damage Induced by Carcinogenic Allyl Isothiocynate"Free Radic.Biol.Med.. 28. 797-805 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Tada-Oikawa: "Determination of DNA damage, peroxide generation, mitochondrial membrane potential, and caspase-3 activity during ultraviolet A-induced apoptosis"Methods Enzymol.. 319. 331-342 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Ito: "Sequence Specificity of Ultraviolet A-induced DNA Damage in the Presence of Photosensitizer"Methods Enzymol.. 319. 417-427 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Oikawa: "Mechanism of Acceleration of Telomere Shortening by UVA"Photomedicine and Photobiology. 22. 47-48 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Yamashita: "Distinct Mechanisms of DNA Damage in the Process of Apoptosis Induction by Quercetin and Luteolin"Free Radic.Res.. 33. 623-633 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Murata: "Oxidative DNA Damage by Minor Metabolites of Toluene May Lead to Carcinogenesis and Reproductive Dysfunction"Biochem.Biophys.Res.Commun.. 261. 478-483 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Ohkuma: "Oxidative DNA Damage by a Metabolite of Carcinogenic and Reproductive Toxic Nitrobenzene in the Presence of NADH and Cu(II)"Biochem.Biophys.Res.Commun.. 257. 555-560 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Kawanishi: "DNA damage induced by Salmonella test-negative carcinogens through the formation of oxygen and nitrogen-derived reactive species (review)"Int.J.Mol.Med.. 3. 169-174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 及川伸二: "外因性内分泌攪乱化学物質(環境ホルモン)問題の現状と今後"労働の科学. 54. 340-344 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 山下成人: "環境エストロゲンと内分泌攪乱"臨床検査. 43. 1375-1382 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 川西正祐: "中毒学 -基礎・臨床・社会医学-"朝倉書店 (荒記俊一 編). 408 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Kawanishi: "Curr Probl Dermatol.Vol.29 Oxidants and Anitoxidants in Cutaneous Biology"Karger(J.Thiele and P.Elsner eds.). 193 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 川西正祐: "別冊 医学のあゆみ 酸化ストレス-フリーラジカル医学生物学の最前線"医歯薬出版 (吉川敏一 編). 326 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Kawanishi: "Free Radicals in Chemistry, Biology and Medicine"OICA International (T.Yoshikawa, S.Toyokuni, Y.Yamamoto and Y.Naito, eds.). 518 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Homma-Takeda S, Hiraku, Y. Ohkuma Y. Oikawa S, Murata M, Ogawa K, Iwamuro T, Li S, Sun GF, Kumagai Y, Shimojo N, Kawanishi S.: "2,4,6-Trinitrotoluene-induced Reproductive Toxicity via Oxidative DNA Damage by its Metabolite."Free Radic Res.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi S, Hiraku Y, Murata M, Oikawa S.: "The role of metals in site-specific DNA damage with reference to carcinogenesis."Free Radic. Biol. Med.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murata M, Kawanishi S.: "Oxidation of 5'-site guanine at GG and GGG sequences induced by a metabolite of carcinogenic heterocyclic amine PhIP in the presence of Cu(II) and NADH."Carcinogenesis. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohnishi S, Murata M, Kawanishi S.: "DNA damage induced by hypochlorite and hypobromite with reference to inflammation-associated carcinogenesis."Cancer Lett.. 178(1). 37-42 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiraku Y, Murata M, Kawanishi S.: "Determination of intracellular glutathione and thiols by high performance liquid chromatography with a gold electrode at the femtomole level : comparison with a spectroscopic assay."Biochim Biophys Acta.. 1570(1). 47-52 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirakawa K, Oikawa S, Hiraku Y, Hirosawa I, Kawanishi S.: "Catechol and hydroquinone have different redox properties responsible for their differential DNA-damaging ability."Chem Res Toxicol.. 15(1). 76-82 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohnishi S, Kawanishi S.: "Double base lesions of DNA by a metabolite of carcinogenic benzo[a]pyrene."Biochem Biophys Res Commun.. 290(2). 778-782 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi S, Hiraku Y, Oikawa S.: "Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging."Mutat Res.. 488(1). 65-76 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi S, Inoue S, Oikawa S, Yamashita N, Toyokuni S, Kawanishi M, Nishino K.: "Oxidative DNA damage in cultured cells and rat lungs by carcinogenic nickel compounds."Free Radic Biol Med.. 31(1). 108-116 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hishita T, Tada-Oikawa S, Tohyama K, Miura Y, Nishihara T, Tohyama Y, Yoshida Y, Uchiyama T, Kawanishi S.: "Caspase-3 activation by lysosomal enzymes in cytochrome c-independent apoptosis in myelodysplastic syndrome-derived cell line P39."Cancer Res.. 61(7). 2878-2884 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oikawa S, Tada-Oikawa S, Kawanishi S.: "Site-specific DNA damage at the GGG sequence by UVA involves acceleration of telomere shortening."Biochemistry. 40(15). 4763-4768 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Midorikawa K, Kawanishi S.: "Superoxide dismutases enhance H_2O_2-induced DNA damage and alter its slte specificity."FEBS Lett.. 495(3). 187-190 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murata M, Ohnishi S, Kawanishi S.: "Acrylonitrile enhances H_2O_2-mediated DNA damage via nitrogen-centered radical formation."Chem Res Toxicol.. 14(10). 1421-1427 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oikawa S, Hirosawa I, Hirakawa K, Kawanishi S.: "Site specificity and mechanism of oxidative DNA damage induced by carcinogenic catechol."Carcinogenesis. 22(8). 1239-1245 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murata M, Tamura A, Tada M, Kawanishi S.: "Mechanism of oxidative DNA damage induced by carcinogenic 4-aminobiphenyl."Free Radic Biol Med.. 30(7). 765-773 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murata M, Bansho Y, Inoue S, Ito K, Ohnishi S, Midorikawa K, Kawanishi S.: "Requirement of glutathione and cysteine in guanine-specific oxidation of DNA by carcinogenic potassium bromate."Chem Res Toxicol.. 14(6). 678-685 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakano K, Oikawa S, Hasegawa K, Kawanishi S.: "Hydroxyurea Induces Site-specific DNA Damage via formation of hydrogen peroxide and nitric oxide."Jpn J Cancer Res.. 92(11). 1166-1174 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohkuma Y, Hiraku Y, Kawanishi S.: "Sequence-specific DNA damage induced by carcinogenic danthron and anthraquinone in the presence of Cu(II), cytochrome P450 reductase and NADPH."Free Radic Res.. 34(6). 595-604 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohnishi S, Murata M, Degawa M, Kawanishi S.: "Oxidative DNA damage induced by an N-hydroxy metabolite of carcinogenic 4-dimethylaminoazobenzene."Jpn J Cancer Res.. 92(1). 23-29 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakano K, Oikawa S, Murata M, Hiraku Y, Kojima N, Kawanishi S.: "Mechanism of metal-mediated DNA damage induced by metabolites of carcinogenic 2-nitropropane."Mutat Res.. 479(1-2). 101-111 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohnishi S, Murata M, Oikawa S, Totsuka Y, Takamura T, Wakabayashi K, Kawanishi S.: "Oxidative DNA damage by an N-hydroxy metabolite of the mutagenic compound formed from norharman and aniline."Mutat Res.. 494(1-2). 63-72 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Midorikawa K, Murata M, Oikawa S, Hiraku Y, Kawanishi S.: "Protective effect of phytic acid on oxidative DNA damage with reference to cancer chemoprevention."Biochem Biophys Res Commun.. 288(3). 552-557 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohkuma Y, Kawanishi S.: "Oxidative DNA damage induced by a metabolite of carcinogenic o-anisidine : enhancement of DNA damage and alteration in its sequence specificity by superoxide dismutase."Arch Biochem Biophys.. 389(1). 49-56 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohnishi S, Murata M, Fukuhara K, Miyata N, Kawanishi S.: "Oxidative DNA damage by a metabolite of carcinogenic 1-nitropyrene."Biochem Biophys Res Commun.. 280(1). 48-52 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiraku Y, Oikawa S, Kuroki K, Sugiyama H, Saito I, Kawanishi S.: "Amplification of bleomycin-induced DNA cleavage at cytosine residues 3' to GGG sequences by pyrrole triamide."Biochem Pharmacol.. 61(3). 351-356 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiraku Y, Yamashita N, Nishiguchi M, Kawanishi S.: "Catechol estrogens induce oxidative DNA damage and estradiol enhances cell proliferation."Int J Cancer. 92(3). 333-337 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi S, Hiraku Y.: "Sequence-specific DNA damage induced by UVA radiation in the presence of endogenous and exogenous photosensitizers."Curr Probl Dermatol.. 29. 74-82 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murata M, Kawanishi S.: "Oxidative DNA damage by vitamin A and its derivative via superoxide generation."J Biol Chem.. 275(3). 2003-2008 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi S, Oikawa S, Kawanishi M, Sugiyama H, Saito I, Strekowski L, Wilson WD.: "Amplification of pepleomycin-mediated DNA cleavage and apoptosis by unfused aromatic cations."Biochemistry. 39(43). 13210-13215 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiraku Y, Kawanishi S.: "Distinct mechanisms of guanine-specific DNA photodamage induced by nalidixic acid and fluoroquinolone antibacterials."Arch Biochem Biophys.. 382(2). 211-218 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohnishi S, Murata M, Oikawa S, Hiraku Y, Kawanishi S.: "Copper-dependent DNA damage induced by hydrazobenzene, an azobenzene metabolite."Free Radic Res.. 32(6). 469-478 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Midorikawa K, Murata M, Oikawa S, Tada-Oikawa S, Kawanishi S.: "DNA damage by dimethylformamide : role of hydrogen peroxide generated during degradation."Chem Res Toxicol.. 13(4). 309-315 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murata M, Yamashita N, Inoue S, Kawanishi S.: "Mechanism of oxidative DNA damage induced by carcinogenic allyl isothiocyanate."Free Radic Biol Med.. 28(5). 797-805 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tada-Oikawa S, Oikawa S, Kawanishi S.: "Determination of DNA damage, peroxide generation, mitochondrial membrane potential, and caspase-3 activity during ultraviolet A-induced apoptosis."Methods Enzymol.. 319. 331-342 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito K, Kawanishi S.: "Sequence specificity of ultraviolet A-induced DNA damage in the presence of photosensitizer."Methods Enzymol.. 319. 417-427 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oikawa S, Tada-Oikawa S, Kawanishi S.: "Mechanism of Acceleration of Telomere Shortening by UVA."Photomedicine and Photobiology. 22. 47-48 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita N, Kawanishi S.: "Distinct mechanisms of DNA damage in apoptosis induced by quercetin and luteolin."Free Radic Res.. 33(5). 623-633 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Murata M, Tsujikawa M, Kawanishi S.: "Oxidative DNA damage by minor metabolites of toluene may lead to carcinogenesis and reproductive dysfunction."Biochem Biophys Res Commun.. 261(2). 478-483 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohkuma Y, Kawanishi S.: "Oxidative DNA damage by a metabolite of carcinogenic and reproductive toxic nitrobenzene in the presence of NADH and Cu(II)."Biochem Biophys Res Commun.. 257(2). 555-560 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi S, Hiraku Y, Inoue S.: "DNA damage induced by Salmonella test-negative carcinogens through the formation of oxygen and nitrogen-derived reactive species (review)."Int J Mol Med.. 3(2). 169-174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kawanishi S, Oikawa S, Hiraku, Y.: "Role of Sequence-specific DNA Damage in Carcinogenesis and Aging. In : Free Radicals in Chemistry, Biology and Medicine, (T. Yoshikawa, S. Toyokuni, Y. Yamamoto and Y. Naito, eds. )"OICA International, London. 85-91 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Ohnishi: "DNA damage induced by hypochlorite and hypobromite with reference to inflammation-associated carcinogenesis"Cancer Lett.. 178. 37-42 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Hirakawa: "Catechol and Hydroquinone Have Different Redox Properties Responsible for Their Differential DNA-damaging Ability"Chem. Res. Toxicol. 15. 76-82 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Ohnishi: "Double Base Lesions of DNA by a Metabolite of Carcinogenic Benzo[a]pyrene"Biochem. Biophys. Res. Commun.. 290. 778-782 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Kawanishi: "Mechanism of Guanine-specific DNA Damage by Oxidative Stress and Its Role in Carcinogenesis and Aging"Mutation Res.(Reviews in Mutation Research). 488. 65-76 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Kawanishi: "Oxidative DNA damage in cultured cells and rat lungs by carcinogenic nickel compounds"Free. Radic. Biol. Med.. 31. 108-116 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Hishita: "Caspase-3 Activation by Lysosomal Enzymes in Cytochrome c-independent Apoptosis in a Myelodysplastic Syndrome(MDS)-derived Cell Line P39"Cancer Res.. 61. 2878-2884 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Oikawa: "Site-specific DNA Damage at GGG Sequence by UVA Involves Acceleration of Telomere Shortening"Biochemistry. 40. 4763-4768 (2001)

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      2001 Annual Research Report
  • [Publications] K.Midorikawa: "Superoxide dismutases enchance H2O2-induced DNA damage and alter its site specificity"FEBS Lett.. 495. 187-190 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Murata: "Acrylonitril Enhances H_2O_2-Mediated DNA Damage via Nitrogen-Centered Radical Formation"Chem. Res. Toxicol.. 14. 1421-1427 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Oikawa: "Site Specificity and Mechanism of Oxidative DNA Damage Induced by Carcinogenic Catechol"Carcinogenesis. 22. 1239-1245 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Murata: "Mechanism of Oxidative DNA Damage Induced by Carcinogenic 4-Aminobihenyl"Free Radic. Biol Med.. 30. 765-773 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Murata: "Requirement of Glutathione and Cysteine in Guanine-specific Oxidation of DNA by Carcinogenic Potassium Bromate"Chem. Res. Toxicol.. 14. 678-685 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Sakano: "Hydroxyurea Induces Site-specific DNA Damage via Formation of Hydrogen Peroxide and Nitric Oxide"Jpn. J. Cancer Res.. 92. 1166-1174 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Ohkuma: "Sequence-specific DNA damage induced by carcinogenic danthron and anthraquinone in the presence of Cu(II), cytochrome P450 reductase and NADPH"Free. Radic. Res.. 34. 595-604 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Ohnishi: "Oxidative DNA damage induced by an N-hydroxy metabolite of carcinogenic 4-dimethylaminoazobenzene"Jpn. J. Cancer Res.. 92. 23-29 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Sakano: "Mechanism of Metal-mediated DNA Damage Induced by Metabolites of Carcinogenic 2-Nitropropane"Mutat. Res.. 479. 101-111 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Ohnishi: "Oxidative DNA Damage by an N-Hydroxy Metabolite of the Mutagenic Compound Formed from Norharman and Aniline"Mutat.Res.. 494. 63-72 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Midorikawa: "Protective Effect of Phytic Acid on Oxidative Damage with Reference to Cancer Chemoprevention"Biochem. Biophys. Res. Commun. 288. 552-557 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Ohkuma: "Oxidative DNA Damage Induced by a Metabolite of Carcinogenic o-Anisidine : Enhancement of DNA Damage and Alteration in its Sequence-Specificity by Superoxide Dismutase"Arch Biochem. Biophys.. 389. 49-56 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Ohnishi: "Oxidative DNA Damage by a Metabolite of Carcinogenic 1-Nitropyrene"Biochem. Biophys. Res. Commun.. 280. 48-52 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Hiraku: "Amplification of Bleomycin-induced DNA Cleavage at Cytosine Residues 3' to GGG Sequences by Pyrrole Triamide"Biochem. Pharmaco. 61. 351-356 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Hiraku: "Catechol Estrogens Induce Oxidative DNA Damage and Estradiol Enhances Cell Proliferation."Int. J. Cancer. 92. 333-337 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 及川伸二: "環境因子による酸化的DNA損傷とがん、老化"Environ. Mut. Res. Commun.. 23. 207-213 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Kawanishi: "Oxidants and Anitoxidants in Cutaneous Biology"J.Thiele and P.Elsner. 193 (2001)

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      2001 Annual Research Report
  • [Publications] 川西正祐: "中毒学 -基礎・臨床・社会医学-"朝倉書店(荒木俊一 編). 408 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 川西正祐: "別冊 医学のあゆみ 酸化ストレス-フリーラジカル医学生物学の最前線"医歯薬出版(吉川敏一 編). 326 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Murata: "Oxidative DNA Damage by Vitamin A and its Derivative via Superoxide Generation"J.Biol.Chem... 275. 2003-2008 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Midorikawa,: "DNA Damage by Dimethylformamide : Role of Hydrogen Peroxide Generated During Degradation"Chemical Research in Toxicology.. 13. 309-315 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Ohnishi,: "Oxidative DNA Damage by a Metabolite of Carcinogenic 1-Nitropyrene"Biochem.Biophys.Res.Commun.. 280. 48-52 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Ohnishi,: "Oxidative DNA Damage Induced by an N-Hydroxy Metabolite of Carcinogenic 4-Dimethylaminoazobenzene"Jpn.J.Cancer Res.. 92. 23-29 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Hiraku,: "Amplification of Bloomycin-induced DNA Cleavage at Cytosine Residues 3' to GGG Sequences by Pyrrole Triamide"Biochemical Pharmacology. 61. 351-356 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Murata,: "Mechanism of Oxidative DNA Damage Induced by Carcinogenic 4-Aminobipehneyl."Free Radical Biol Med.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Y. Ohkuma: "Oxidative DNA Damage by a Metabolite of Carcinogenic and Reproductive Toxic Nitrobenzene in the Presence of NADH and Cu(II)"Biochem. Biophys. Res. Commun.. 257. 555-560 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] M. Murata: "Oxidative DNA Damage by Minor Metabolites of Toluene May Lead to Carcinogenesis and Reproductive Dysfunction"Biochem. Biophys. Res. Commun.. 261. 478-483 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Ohkuma: "Distinct Mechanisms of Oxidative DNA Damage by Two Metabolites of Carcinogenic o-Toluidine"Arch. Biochem. Biophys.. 372. 97-106 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 及川伸二: "外因性内分泌攪乱化学物質(環境ホルモン)問題の現状と今後"労働の科学. 54. 340-344 (1999)

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  • [Publications] 山下成人: "環境ホルモンと性内分泌攪乱"臨床検査. 43. 1375-1382 (1999)

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Published: 2001-04-01   Modified: 2016-04-21  

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