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Development of the artificial organs from human stem cells.

Research Project

Project/Area Number 11794032
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionAkita University

Principal Investigator

SUGIYAMA Toshihiro  Akita University, School of Medicine, Professor, 医学部, 教授 (00127242)

Co-Investigator(Kenkyū-buntansha) TERADA Kunihiko  Akita University, School of Medicine, Associate Professor, 医学部, 助教授 (60197796)
KATO Yukio  Hiroshima University, School of Dentistry, Professor, 歯学部, 教授 (10112062)
MANABE Motomu  Akita University, School of Medicine, Professor, 医学部, 教授 (30138309)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2001: ¥14,500,000 (Direct Cost: ¥14,500,000)
KeywordsTissue Engineering / Liver regeneration / Stem Cells / Cultured skin / Shh / Mesenchyme / Bone regeneration / フィーダー細胞 / ケラチノサイト / 肝臓幹様細胞 / 毛幹細胞 / 骨髄間葉系細胞 / 骨再生 / 毛再生 / 発毛促進
Research Abstract

On this research, we tried to develop new technologies to regenerate the liver, skin-hair, and cartilagebone from the adult tissue stem cells.
1. Liver. We established several cell clones from the liver epithelium and succeeded to induce the differentiation of them to the hepatocytes in vitro. We also tried and partially succeeded to recover the liver function on the experimental animals by using these cells.
2. Skin-Hair. We developed a new-technology to accelerate the formation of the cultured epithelium by using Sonic hedgehog expressing feeder cells. This result indicates that the application of the knowledge from the molecular biology is useful to develop the tissue engineering.
3. Mesenchyme. We obtained mesenchymal stem cells from the human bone marrow and succeeded to differentiate them to the osteoblast, chondrocytes, and adipocytes. We are trying to apply these cells to the plastic surgery.
The connective research among regions was very powerful and useful for the combined and prompt research.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (68 results)

All Other

All Publications (68 results)

  • [Publications] 寺田邦彦、他: "肝幹細胞"Annual Review消化器2002. 34-39 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanzaki A, et al.: "Copper-transporting P-Type Adenosine Triphosphatase(ATP7B)Is Expressed in Human Breast Carcinoma"Jpn.J.Cancer Res.. 93. 70-77 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kameda T, et al.: "Acceleration of the formation of cultured epithelium using the sonic hedgehog expressing feeder cells"Tissue Eng.. 7. 545-555 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M, et al.: "A Mutation of Wilson's Disease Protein,ATP7B,Is Degraded in the Proteasomes and Forms Protein Aggregates"Gastroenterology. 120. 967-974 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Demitsu T, et al.: "Hypertrichosis induced by latanoprost"J.Am.Acad.Dermatol.. 44. 721-723 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichihara T, et al.: "Resistance to fluminant hepatitis and carcinogenesis conferred by overexpression of retinoblastoma protein in mouse liver"Hepatology. 33. 948-955 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺田邦彦、他: "肝幹細胞を利用した肝組織の再構成"医学のあゆみ. 196. 383-386 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺田邦彦、他: "幹細胞を用いたミニ肝臓の形成"GI Research. 93. 70-77 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺田邦彦、他: "幹細胞を用いたミニ肝臓の形成"Molecular Medicine. 38. 16-22 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺田邦彦、他: "肝臓幹細胞とその分化"肝臓. 42. 389-397 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M, et al.: "Role of ATP7B in Biliary Copper Excretion in a Human Hepatoma Cell Line and Normal Rat Hepatocytes"Gastroenterology. 118. 921-928 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Komatsu M, et al.: "Copper transporting P-type adenosine triphosphatase(ATP7B)is associated with Cisplatin resistance"Cancer Res.. 60. 1312-1316 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujita K, et al.: "Quantitative detection of apoptotic thymocytes in low-dose X-irradiated mice by an anti-single-stranded DNA antibody"J.Radiat.Res.. 41. 139-149 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M, et al.: "Copper does not alter the intracellular distribution of ATP7B,a copper-transporting ATPase"Biochem.Biophys.Res.Commun.. 275. 871-876 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang X.L., et al.: "MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2Cl2 myoblasts"FEBS Letters. 470. 29-34 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Narisawa T, et al.: "Inhibitory effects of ursodeoxycholic acid on N-methyl-nitrosourea-induced colon carcinogenesis and colonic mucosal telomerase activity in F344 rats"J.Exp.Clin.Cancer Res.,18:259-266,1999. 18. 259-266 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Biliary expression of copper in LEC rat after introduction of copper transporting P-type ATPase,ATP7B"FEBS Lett.. 448. 53-56 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "The Long-Evans Cinnamon rat: An animal model for Wilson's disease"Pediatr.Int.. 41. 414-418 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugiyama T, et al.: "Reconstitution of hepatic tissue using liver stem cells"Growth,proliferation,and apoptosis in hepatocytes(Okita K.,ed.),Springer-Verlag,Tokyo. 100 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺田邦彦、他: "組織工学的手法を利用したミニ肝臓の作製"消化器疾患の最新医療(森岡恭彦、他編).先端医療技術研究所.東京. 663 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺田邦彦、他: "肝幹細胞と毛幹細胞"化学フロンティア(3)再生医工学(筏義人、編).化学同人.京都. 254 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Hepatic stem cell"Annual review of digestive disease in 2002. 34-39 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kanzaki A, et al.: "Copper-transporting P-type adenosine triphosphatase (ATP7B) is expressed in human breast carcinoma."Jpn. J. Cancer Res.. 93. 70-77 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kameda T, et al.: "Acceleratioh of the formation of cultured epithelium using the sonic hedgehog expressing feeder cells."Tissue Eng.. 7. 545-555 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M, et al.: "A mutation of Wilson's disease protein, ATP7B, is degraded in the proteasomes and forms protein aggregates."Gastroenterology. 120. 967-974 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Demitsu T, et al.: "Hypertrichosis induced by latanoprost."J. Am. Acad. Dermatol.. 44. 721-723 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ichihara T, et al.: "Resistance to fluminant hepatitis and carcinogenesis conferred by overexpression of retinoblastoma protein in mouse liver."Hepatology. 33. 948-955 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Reconstitution of hepatic tissue using hepatic stem cells."IGAKU NO AYUMI. 196. 383-386 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Construction of mini-liver using hepatic stem cells."GI Research. 93. 70-77 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Construction of mini-liver using hepatic stem cells."Molecular Medicine. 38. 16-22 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Hepatic stem cell and its differentiation."Acta Hepatologica Japonica. 42. 389-397 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M, et al.: "Role of ATP7B in biliary copper excretion in a human hepatoma cell line and normal rat hepatocytes."Gastroenterology. 118. 921-928 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Komatsu M, et al.: "Copper transporting P-type adenosine triphosphatase (ATP7B) is associated with Cisplatin resistance."Cancer Res.. 60. 1312-1316 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujita K, et al.: "Quantitative detection of apoptotic thymocytes in low-dose X-irradiated mice by an anti-single-stranded DNA antibody."J. Radiat. Res.. 41. 139-149 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M, et al.: "Copper does not alter the intracellular distribution of ATP7B, a copper-transporting ATPase."Biochem. Biophys. Res. Commun.. 275. 871-876 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang X. L., et al.: "MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2C12 myoblasts."FEBS Letters. 470. 29-34 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Narisawa T, et al.: "Inhibitory effects of ursodeoxycholic acid on N-methyl-nitrosourea-induced colon carcinogenesis and colonic mucosal telomerase activity in F344 rats."J. Exp. Clin. Cancer Res.. 18. 259-266 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Biliary expression of copper in LEC rat after introduction of copper transporting P-type ATPase, ATP7B."FEBS Lett.. 448. 53-56 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "The Long-Evans Cinnamon rat : An animal model for Wilson's disease."Pediatr. Int.. 41. 414-418 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugiyama T, et al.: "Reconstituteion of hepatic tissue using liver stem cells. In Growth , Proliferation, and Apoptosis in Hepatocytes (Okita K., ed.)"Springer-Verlag, Tokyo. 100 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Construction of mini-liver by tissue engineering techniques. In Advenced Medicine for Digestive Diseases (Morioka Y., et al., eds.)"Institute of Advanced Medical Techniques, Tokyo. 663 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Terada K, et al.: "Hepatic stem cell and hair stem cell. In KAGAKU Frontier 3, Regenerative Medical Technology (Ikada Y., ed.)"KAGAKU DOJIN, Kyoto. 254 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 寺田邦彦, 他: "肝幹細胞"Annual Review 消化器2002. 34-39 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanzaki A, et al.: "Copper-transporting P-Type Adenosine Triphosphatase (ATP7B) Is Expressed in Human Breast Carcinoma"Jpn.J.Cancer Res.. 93・1. 70-77 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kameda T, et al.: "Acceleration of the formation of cultured epithelium using the sonic hedgehog expressing feeder cells"Tissue Eng.. 7・5. 545-555 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Harada M, et al.: "A Mutation of Wilson's Disease Protein, ATP7B, Is Degraded in the Proteasomes and Forms Protein Aggregates"Gastroenterology. 120・4. 967-974 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Demitsu T, et al.: "Hypertrichosis induced by latanoprost"J.Am.Acad.Dermatol.. 44・4. 721-723 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ichihara T, et al.: "Resistance to fluminant hepatitis and carcinogenesis conferred by overexpression of retinoblastoma protein in mouse liver"Hepatology. 33・4. 948-955 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 寺田邦彦, 他: "肝臓幹細胞とその分化"肝臓. 42. 389-397 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 寺田邦彦, 他: "肝幹細胞を利用した肝組織の再構成"医学のあゆみ. 196. 383-386 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 寺田邦彦, 他: "幹細胞を用いたミニ肝臓の形成"G.I.Research. 9. 51-55 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 寺田邦彦, 他: "幹細胞を用いたミニ肝臓の形成"Molecular Medicine. 38. 16-22 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sugiyama T, et al.: "Reconstitution of hepatic tissue using liver stem cells."Growth, proliferation, and apoptosis in hepatocytes (Okita K., ed.), Springer-Verlag, Tokyo.. 100 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Harada M.: "Role of ATP7B in Biliary Copper Excretion in a Human Hepatoma Cell Line and Normal Rat Hepatocytes."Gastroenterology. 118. 921-928 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yang X.L.: "MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2C12 myoblasts."FEBS Letters. 470. 29-34 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Komatsu M.: "Copper transporting P-type adenosine triphosphatase (ATP7B) is associated with Cisplatin resistance."Cancer Res.. 60. 1312-1316 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nagai H: "Genomic DNA analyses of spontaneous hepatocellular carcinomas in LEC rat liver using a new technique."Oncology Report. 7. 315-318 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 寺田邦彦: "幹細胞を用いたミニ肝臓の形成"Molecular Medicine. 38. 16-20 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 寺田邦彦: "肝幹細胞を利用した肝組織の再構成"医学のあゆみ. 196. 383-386 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 寺田邦彦: "『再生医療工学』"化学同人(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugiyama T.: "Experimental protocols for reactive oxygen and nitrogen species"Oxford University Press, New York. 400 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawashima-Ohya,Y.,et al.: "Retinol-binding protein is produced by rabbit chondrocytes and responds to parathyroid hormone (PHT)/PTH-related peptide-cyclic adenosine monophosphate pathway"Endocrinology. 140. 1075-1081 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Makihira,S.,et al.: "Enhancement of cell adhesion and spreading by a cartilage-speciffic noncollagenous protein,cartilage matrix protein (CMP/Matrilin-1),via integrin a1b1"J.Biol.Chem.. 274. 11417-11423 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujimoto,E.,et al.: "Beneficial effect of basic fibroblast growth factor on the repair of full-thickness defects in rabbit articular cartilage"Arch.Orthop.Trauma Surg. 119. 139-145 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Harada M.et al.: "Wilson's disease gene product,ATP7B,localizes in the late endosomes in a human hepatoma cell line and normal rat hepatocytes : Participation in biliary copper"Gastroenterology. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Terada K.et al.: "Biliary expression of copper in LEC rat after introduction of copper transporting P-type ATPase,ATP7B"FEBS Lett.. 448. 53-56 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Terada K.et al.: "The Long-Evans Cinnamon rat : An animal model for Wilson's disease"Pediatr.Int.. 41. 414-418 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugiyama,T.et al.: "Liver stem-like(oval)cells : isolotion,differentiation and application for cell therapy. In Ikada,Y.and Okano,T.ed. Tissue Engineering for Therapeutic Use"Elsevier Science B.V.. 161 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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