| Project/Area Number |
11794036
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Tokai University |
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Principal Investigator |
HOTTA Tomomitsu Tokai University, School of Medicine, Professor, 医学部, 教授 (70173606)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Takashi JT pharmaceutical co., Chief researcher, 主任研究員 (50339131)
KATO Shunichi Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (70096212)
ANDO Kiyoshi Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (70176014)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2001: ¥13,000,000 (Direct Cost: ¥13,000,000)
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| Keywords | Cord blood / ex vivo expansion / stromal cell / hematopoietic stem cell / CD34+cell / 造血幹細胞移植 / マウス骨髄ストローマ細胞株 / 膜分離型共培養系 / サイトカイン / 臍帯血、 / 造血幹細胞、 / 体外増幅、 / 造血幹細胞移植、 / 膜分離型共培養系、 |
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| Research Abstract |
Recently, cord blood has been calling attention as a third source for supplying hematopoietic stem cells, next to bone marrow and peripheral blood. However, the biggest problem with cord blood is its limitation : the amount of hematopoietic stem cells contained, which restricts the administration of cord blood to adults. We developed a unique system for culture in which cord blood and stromal cells are separated by membrane, but still keep contact through pores with 0.45 micrometer. We were able to expand cord blood stem cells with this system in experiments. For the clinical application, we have to attain 1) control of the contamination of xenogeneic proteins and micro-organisms derived from murine stromal cell line, 2) sacale up the culture system, and 3) evaluation of the engraftment and long-term sustain of hematopoiesis. For these purposes we ordered the bioventure company, Bioreliance Co., to check the contamination of xenogeneic proteins and micro-organism in the reference of the guideline from FDA and confirmed the murine stromal cell line, HESS-5, was free from xenogeneic micro-organism. We also developed the large scale culture system by using membrane of 100 cm2. We evaluated the expansion of SRC by using of quantitative SRC assay, and confirmed more than 10 times expansion of SRC within 5 days of culture. We started clinical protocol after the disussion in IRB.
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