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Effect of magnetic field on the intracellular environment

Research Project

Project/Area Number 11834006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionOsaka University

Principal Investigator

NAKAOKA Yasuo  Graduate School of Engineering Science, Osaka University Associate Professor, 基礎工学研究科, 助教授 (90029562)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsMagnetic field / Paramecium / diamagnetism / cilia / Membrane current / Trichoeyst / 蛋白 / 遊泳行動 / 直流磁場 / 交流磁場 / 細胞内カルシウムイオン / 膜電位
Research Abstract

In order to reveal the physiological effect of magnetic field on the living organisms, we applied the magnetic field to the protozoan, Paramecium.
First, application of AC magnetic field (60 Hz, 0.6 T) induced Paramecium cells to accumulate remarkably to upper part of the suspension. Swimming tracks of the cells accumulating to upper side showed that most cells gradually change the swimming direction to orient to upward one. This result indicates the possibility that the AC magnetic field strengthens the negative gravitaxis which is caused by sensing the gravity. How the sensitivity to gravity is increased by the AC magnetic field must be studied further.
Next, when Paramecium cells were placed in a DC magnetic field (0.7 T), the cells began to swim perpendicularly to the magnetic field. Such response is supposed to be originated by a diamagnetism of intercellular organs. The diamagnetism is increased with the regular arrangements of proteins or nucleotides. Then, macronuclei, cilia and trichocyst were extracted from the cell and the orientation of those organs in DC magnetic field were tested. Although macronuclei did not change the orientation, both cilia and trichocyst changed the orientation to direct almost parallel to the magnetic field. Cilia and trichocyst distribute on the surface membrane and most parts of them direct perpendicularly to the long axis of cell. Such situation causes the cell to swim in perpendicular to the magnetic field. It is concluded that the response of Paramecium against DC magnetic field is essentially originated from diamagnetism of intracellular organs.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Oka,T: "Differentiation of rat thymic mycid progenitor cell line established by coculture with human T-lymphotrophicvirus"Cell Tissue Res.. 300. 119-127 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murata,T.: "Procstaglandin I_2, a possible therme-sensory mediator in Paramecium"Biockem.Biophys.Res.Commun.. 273. 516-520 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakaoka,Y.: "Effect of 60Hz magnetic field on the behavior of Paramecium"Bioelectromagnetics. 21. 584-588 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Iwamoto,M.: "Activation of Prostacyclin synthesis by mechanical stimulation in the ciliated Protogoau Tetrahymena."Biochem.Biophys.Res.Commun.. 275. 635-638 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hasegawa, K., Ishizaki, S., Tsukahara, Y., Nakaoka Y.and Miwa, I.: "Circadian rhythms in Paramecium."Trends in Photochem.Photobiol.. 6. 75-98 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Oka, T., Hayashi, K., Nakaoka, Y., Ohtsuki, Y.and Akagi, T.: "Differentiation of rat thymic myoid progenitor cell line established by coculture with human T-lymphotropic virus type-I-producing human T cells."Cell Tissue Res.. 300. 119-127 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murata, T., Morii, H., Watanabe, Y., Matsumura, K.Watanabe, Y.and Nakaoka, Y.: "Prostaglandin I_2, a possible thermo-sensory mediator in Paramecium."Biochem.Biophys.Res.Commun.. 273. 516-520 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakaoka, Y., Shimizu, K., Hasegawa, K.and Yamamoto, T.: "Effect of a 60Hz magnetic field on the behavior of Paramecium"Bioelectromagnetics. 21. 584-588 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Iwamoto, M., Murata, T., Morii, H., Watanabe Y.and Nakaoka, Y.: "Activation of prostacyclin synthesis by mechanical stimulation in the ciliated protozoan Tetrahymena thermophila."Biochem.Biophys.Res.Commun.. 279. 635-638 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Oka,T.: "Differentiation of rat thymic myoid progeritor cell line established by coculture with human T-lymphotropievirus"Cell Tissue Res.. 300. 119-127 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murata,T.: "Prostaglardin I_2, a possible thermp-sensory medcator in Paramecium"Biochem Biophys.Res.Commun. 273. 516-520 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakaoka,Y.: "Effect of a 60Hz magnetic field on the behavior of Paramecium"Bioelectromagnetico. 21. 584-588 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Iwamoto,M.: "Activation of prostacyclin synthesis by mechanical stimulation in the ciliated protozoan Tetrahymena"Biochem.Biophys.Res.Commun.. 275. 635-638 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Hasegawa: "Circadian rhythms in Paramecium"Trends in Photochem. Photobiol. 6. 75-98 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Oka: "Ditterentiation of rat thymus-derived progenitor cell line ectablighed by coculture with HTLZJ T cells"Cell & Tissue Res.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Nakaoka: "Eftect of 60 Hz magnetic field on the behavior of Paramecium"Bioelectromagnetics. (in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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