Correlation between bovine viral diarrhea virus E1 gene and host genome
| Project/Area Number |
11836002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YAMATO Osamu Hokkaido Univ., Grad.Sch.of Vet.Med., Lec., 大学院・獣医学研究科, 講師 (80261337)
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| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Bovine viral diarrhea mucosal disease / BVD / BVDV / Virus antigen / virus gene / BVD-MD / gp25 / E1 |
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| Research Abstract |
Persistently infected cattle with bovine viral diarrhea virus (BVDV) excrete the virus continuously without any clinical signs, and are serious sources of the infection of BVDV in a herd. In order to diagnose the BVDV infection accurately and rapidly, polymerase chain reaction (PCR) has been utilized. By the use of specific primers for detecting p14 and E1 coding regions of BVDV gene, it was possible to discriminate the viral sero types based on the PCR-amplification patterns. However, PCR-amplification patterns of several field isolates from Hokkaido did not correspond to any Japanese BVDV strains. In this study, to clarify the relation between PCR-amplification patterns and viral sero types, a new primer coding the gp25 region of BVDV gene was designed and PCR was performed on Japanese BVDV strains, field isolates, and bovine leucocytes. Then nucleotide sequences and deduced amino acid sequences of the PCR products were compared. Nucleotide sequences and deduced amino acid sequences of the PCR products were highly homologous each other. Therefore E1 seemed to be not concerned in different PCR-amplificatiion pattern. The analysis of amino acid sequences indicated that the hydrophobicity of E1 was highly conserved among each PCR products examined in this study. This region might be a transmembrane domain of E1, and have a function as an anchor for E2 and E0 which induces neutralizing antibodies.
Additionally, highly homologous gene with E1 of BVDV was detected from bovine genome in leucocytes. This gene was recognized in not only BVDV infected cattle but also in uninfected cattle. The mRNAs of this gene were also detected from all cattle examined. This gene might play an important role in attachment or invasion of BVDV to host cell.
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Report
(3 results)
Research Products
(6 results)
