| Project/Area Number |
11836004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | IWATE UNIVERSITY |
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Principal Investigator |
MIYAKE Yoh-ichi FAC.OF AGRICULTURE IWATE UNIVERSITY PROFESSOR, 農学部, 教授 (20002256)
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| Co-Investigator(Kenkyū-buntansha) |
OSAWA Takeshi FAC.OF AGRICULTURE IWATE UNIVERSITY INSTRUCTOR, 農学部, 助手 (90302059)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | mares / granulosa-theca cell tumor / ovaries / endocrinological changes / sex steroid hormones / histohemical staining / inhibin / return to estrus / Pro-αC / インヒビンA / インヒビンB / 超音波画像診断 / インヒビンα鎖 / インヒビンβA鎖,βB鎖 |
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| Research Abstract |
The clinical, structural and functional characteristics of granulosa-theca cell tumor (GTCT) were studied in mares. Localization of the inhibin, inhibin βA, inhibin βB and aromatase were determined in GTCT affected mares by immunohistochemically. Estrogen, testosterone, progesterone, FSH and ir-INH were determined by RIA.
All animals had large abnormal ovary and small ovary in contralateral side by ultrasonographic diagnosis.
All animals had higher ir-INH as compared with that of after removal of the affected ovary, and there were no significantly variation between before and after operation. On the contraraterary, plasma concentration of FSH valies tended to be low in cases as compared with that of after removal of the affected ovary (p<0.05). But plasma concentration of estradiol level tended to be higher in cases as compared with that of after removal of the affected ovary (p<0.05).
According to the results of immunohistochemical staining we can see that INH α was secreted in some of the affected mares. But it was not shown staining patterns of INH-βA, INH-βB and aromatase.
In this studies, it is clear that some of the affected mares can produce dimeric INH and some of the affected mares can not produce dimeric INH.
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