• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of pathological processes in nephritis, associated with systemic infectious response syndrome (SIRS) in dogs and cats.

Research Project

Project/Area Number 11836006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionYamaguchi University

Principal Investigator

ONISHI Takafumi  Faculty of Agriculture, Yamaguchi-University, Professor., 農学部, 教授 (60081593)

Co-Investigator(Kenkyū-buntansha) OHNO Koichi  Graduate School of Agricultural and Life Sciences, University of Tokyo, Associate Professor., 大学院・農学生命科学研究科, 助教授 (90294660)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsserum amyloid A (SAA) / C-reactive protein (CRP) / CTLA-4 / CD 28 / IL-4 / anti neutrophil cytoplasmicantibody (ANCA) / cat (feline) / dog (canine) / CD29 / インターロイキン-4
Research Abstract

(1) Developing a method for measuring the concentration of serum amyloid A (SAA) used as ainflammatory marker
We have successfully constructed a highly expressing recombinant feline (rf) SAA expression vector which expresses feline (f) SAA in Escherichia coli. We have also purified the rfSAA (reference paper 5), as it was difficult to obtain large amount of native SAA for our purpose. Subseqeuently, a panel of monoclonal antibodies specific for fSAA have been prepared by using the rfSAA (reference paper 1). Then an ELISA technique employing the monoclonal antibodies was developed and used in the estimation of fSAA.The results showed that fSAA was able to be used as an inflammatory marker, and is highly usefut for understanding the pathological state of inflammatory diseases (unpublished data).
(2) Usage of T-cell costimulatorymolecules and cytokines related to inflammatory diseases in dogs and cats.
Canine and feline T-cell costimulatory molecules, CTLA-4 and CD28 (reference paper 2, 3, 6 … More ), and a cytokine, IL-4 (reference paper 7), which are related to inflammatory diseases, such as autoimmune diseases, allergies etc, have been cloned and the proteins expressed (unpublished data). The application of these molecules both in reseach as well as for clinical purposes is in study.
(3) Development of a method for detecting anti neutrophil cytoplasmic antibody (ANCA) used as a vasculitis marker in small animals.
We have found that ANCA can be detected in various diseases of dogs and cats by using Indirect Fluorescent Antibody Test and ELISA (presentation 1, 2). The method for detecting ANCA is being developed, based on the previous results, as described in Section 2.
(4) Understanding of the relationship between T-cell costimulatory molecules, cytokines, acute phase proteins (SAA, C-reactive protein), and ANCA.
The dynamic changes of acute phase proteins in diseased dogs and cats have been analyzed, which is an important indicator of disease status (reference paper 4 and 8 unpulished). In future, the relationship between the above mentioned molucules will be described, from the results of section 1, 2 and 3, which will be highly useful in clinical applications. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Khatlani,S.T., et all: "Molecular cloning and sequencing of canine T-cell costimulatory molecule (CD28)"Vet.Immunol.Immunopath.,. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasaki,K., et all: "Characterization of monoclonal antibodies specific for feline serum amyloid A (SAA) protein"Hybridoma,. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Khatlani,S.T., et all: "Cloning and sequencing of canine cDNA encoding the T-cell costimulatory molecule (CTLA-4)"Immunogenetics. 51. 79-81 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 大西堂文 その他: "レーザー免比ろう法による健常犬および疾患犬におけるC-反応性蛋白の測定"日本獣医師会誌. 5・9. 595-601 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohno,K. et all: "Expression of recombinantfeline serum amyloid A (SAA) protein"J.Vet.Med.Sci.. 61・8. 915-920 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohno,K. et all: "Cloning of feline cDNA encoding the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)"J.Vet.Med.Sci.. 61・11. 1241-1244 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Khatlani,S.T. et all: "Cloning of full length cDNA encoding canine interleukin-4"J.Vet.Med.Sci.. 61・8. 967-969 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kajikawa,T. et all: "Changes in concentration of serum amyloid A protein.X1-acid glycoprotein.Haptpglobin.and C-reactiye protein in feline sera due to induced inflammation and surgery."Vet.Immunology and Immunopathology. 68. 91-98 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Khatlani, S.T., et all: "Molecular cloning and sequencing of canine T-cell costimulatory molectule (CD28)"Vet.Immunol.Immunopath. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasaki, K., et all: "Characterization of monoclonal antibodies specific for feline serum amyloid A (SAA) protein"Hybridoma. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Khatlani, S.T., et all: "Cloning and sequencing of canine cDNA encoding the T-cell costimulatoty molecule (CTLA-4)"Immunogenetics. 51. 79-81 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Onishi, T., et all: "C-reactive protein concentrations in normal and diseased dogd measured by laser nephelomic immunoassay"J.Jpn.Vet.Med.Assoc.. 5-9. 595-601 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohno, K.et all: "Expression of recombinant feline serum amyloid A (SAA) protein"J.Vet.Med.Sci.. 61-8. 915-920 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohno, K.et all: "Cloning of feline cDNA encoding the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)"J.Vet.Med.Sci.. 61-11. 1241-1244 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Khatlani, S.T.et all: "Cloning of full length cDNA encoding canine interleukin-4"J.Vet.Med.Sci.. 61-8. 967-969 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kajikawa, T.et all: "Changes in concentration of serum amyloid A protein, xl-acid glycoprotein, Haptpglobin, and C-reactive protein in feline sera due to induced inflammation and surgery."Vet.Immunol.Immunopath.. 68. 91-98 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Khatlani,S.T., et all: "Molecular cloning and sequencing of canine T-cell costimulatory molecule(CD28)"Vet.Immunol.Immunopath.,. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sasaki,K., et all: "Characterization of monoclonal antibodies specific for feline serum amyloid A(SAA)protein"Hybridoma,. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Khatlani,S.T., et all: "Cloning and sequencing of canine cDNA encoding the T-cell costimulatory molecule(CTLA-4)"Immunogenetics. 51. 79-81 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大西堂文 その他: "レーザー免比ろう法による健常犬および疾患犬におけるC-反応性蛋白の測定"日本獣医師会誌. 5・9. 595-601 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.OHNO,M.TERADO,H.IWATA,H.INOKUMA,T.ONISHI: "Expression of Recombinant Feline Serum Amyloid A(SAA) Protein"J.Vet.Med.Sci.. 61(8). 915-920 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.S.KHATLANI,K.OHNO,Z.MA,H.INOKUMA,T.ONISHI: "Cloning of a Full Length cDNA Encoding Canine Interleukin-4"J.Vet.Med.Sci.. 61(8). 967-969 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.OHNO,M.FUJIKI,T.S.KHATLANI,H.INOKUMA,T.ONISHI: "Cloning of Feline cDNA Encoding the Cytotoxic T Lymphocyte-Associated Antigen 4(CTLA-4)"J.Vet.Med.Sci.. 61(11). 1241-1244 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi