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Morphological Variation of Higher Order Structure of Long Duplex DNA and its genetic activity

Research Project

Project/Area Number 11837019
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionCollege of Nagoya Bunri University

Principal Investigator

YOSHIKAWA Yuko  Nagoya Bunji College, assosicate professor, 食物栄養学科, 助教授 (80291871)

Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordslong duplex DNA / higher order structure / fluorescence microscopy / histone H1 / laser manipulation / レーザー / ヒストンH1
Research Abstract

Compaction of DNA is a common feature in native genomes, e.g., in chromatin or phage heads. The manner of packaging is expected to play an essential role in the biological functions of DNA, such as replication and transcription.
I have made direct observations on the conformational change of individual long duplex DNA molecules using fluorescence microscopy. It has been shown that giant DNAs exhibits an all-or-none change, or switching, in the folding transition. I have also reported that complexes of DNA-histone HI form a pearl necklace-like structure when the complexes were prepared by a natural dilution from a high salt solution to a low salt solution. In addition, we have applied laser-tweezers to manipulate the DNA-histone H1 under the real time observation with fluorescence microscopy. When the DNA-H1 complex is optically transported from a low-salt solution to a high-salt solution, its conformation changes from a compact to an elongated structure. With the reverse procedure, the elongated chain shrinks into a compact state. Laser manipulation is expected to be a powerful tool for research on the dynamics of various biomolecules mimicking intracellular processes.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Yuko Yoshikawa: "Formation of a Giant Toroid from Long Duplex DNA"Langmuir. 15. 4085-4088 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuko Yoshikawa: "Folding Transition of Long Duplex DNA from Mammalian Cells"Nucleic Acids Symp.Ser. 42. 61-62 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuko Yoshikawa: "Salt-Dependent Folding of Long Duplex DNA by Histone H1"Nucleic Acids Symp.Ser. 44. 293-294 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuko Yoshikawa: "Controlling the Folding/Unfolding Transition of DNA-Histone H1 Complex by Direct Optical Manipulation"Chem.Phys.Lett. 330. 77-82 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuko Yoshikawa: "Self-assembled pearling structure of long duplex DNA with histone H1"Eur.J.Biochem.. 268. 2593-2599 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shin-ichirou M.Nomura: "Towards proto-cells : primitive lipid vesicles encapsulating giant DNA and Its histone complex"ChemBioChem.. 6. 457-459 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kenichi Yoshikwa: "All-or-none folding transition in giant mammalian DNA"Chem.Phys.Lett.. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y, Yoshikawa, K, Yoshikawa and T, Kanbe: "Formation of a Giant Toroid from Long"Langmuir. 15. 4085-4088 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y, Yoshikawa, T, Kanbe and K, Yoshikawa: "Folding Transition of Long Duplex DNA from Mammalian Cells"Nucleic Acids Symp. Ser.. 42. 61-62 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y, Yoshikawa and T, Kanbe: "Dependent Folding of Long Duplex DNA by Histone H1"Nucleic Acids Symp. Ser.. 44. 293-294 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y, Yoshikawa, S, M, Nomura, T, Kanbe and K, Yoshikawa: "Controlling the Folding/Unfolding Transition of DNA-Histone H1 Complex by Direct Optical Manipulation"Chem. Phys. Lett.. 330. 77-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S, M, Nomura, Y, Yoshikawa, K.Yoshikawa, O, Dannenmuller, S, Chasserot-Golaz, G, Ourisson and Y, Nakatani: "Towards Proto-Cells : Primitive Lipid Vesicles EncapsulatingGiant DMA and ItsHistone Complex"ChemBioChem. 6. 457-459 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y, Yoshikawa, Y, S, Velichko, Y, Ichiba and K, Yoshikawa: "Self-Assembled Pearling Structure of Long Duplex DNA with HistoneHI1"Eur. J. Biochem.. 268. 2593-2599 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K., Yoshikaawa, Y, Yoshikawa and T, Kanbe: "All-or-None Folding Transition in GiantMammalian DNA"Chem. Phys. Lett.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yuko Yoshikawa: "Self-assembled pearling structure of long duplex DNA with histone H1"Eur. J. Biochem. 268. 2593-2599 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shin-ichirou, M.Nomura: "Towards proto-cells : primitive lipid vesicles encapsulating giant DNA and Its histone complex"Chem Bio Chem.. 6. 457-459 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kenichi Yoshikwa: "All-or-none folding transition in giant mammalian DNA"Chem. Phys. Lett.. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yuko Yoshikawa: "Controlling the folding/unfolding transition of DNA-histone H1 complex by direct optical manipulation"Chem.Phys.Lett.. 330. 77-82 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yuko Yoshikawa: "Salt-dependent folding of long duplex DNA by histone H1"Nucleic Acids Symp.Ser.. 44. (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yuko Yoshikawa: "Formation of a giant toroid from long duplex DNA"Langmuir. 15. 4085-4088 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuko Yoshikawa: "Folding transition of long duplex DNA from mammalian cells"Nucleic Acids Symp.Ser.. 42. 61-62 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuko Yoshikawa: "Light illumination induces large change in the higher order structure of giant DNAs complexed with maltosylated amino-pendant polyethylene glycol and sodium dodecyl sulfate"Polym.J.. 31. 1025-1028 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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