• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of the small GTP binding protein, rho, in the vascular smooth muscle.

Research Project

Project/Area Number 11838013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

NISHIMURA Junji  KYUSHU UNIVERSITY, GRADUATE SCHOOL OF MEDICAL SCIENCES, ASSOCIATE PROFESSOR, 大学院・医学研究院, 助教授 (90237727)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsVASCULAR SMOOTH MUSCLE / SMALL GPROTEIN / CALCIUM SENSITIVITY / VASCULAR TONE / CALCIUM / MYOSIN PHOSPHATASE
Research Abstract

The small G protein, rho, has been shown to inhibit myosin light chain phosphatase (MLCP) through the phosphorylation of this enzyme by rho kinase. It was thus obvious that MLCP may be the key protein for the rho-mediated signal transduction to induce modulation of calcium sensitivity of the vascular smooth muscle. MLCP is composed of three subunits, namely, catalytic subunit, small regulatory subunit and large regulatory subunit. We focused on the function of two regulatory subunits of MLCP on the smooth muscle contractile apparatus in the present research program. The major findings were as follows.
1. The effects of the small regulatory subunit of myosin light chain phosphatase (MLCPsr) on the Ca^<2+>-induced contraction of smooth muscle were investigated in the Triton X-100-permeabilized porcine renal artery. The full-length recombinant chicken MLCPsr obtained by the bacterial expression system induced an additional contraction at a constant [Ca^<2+>] i and shifted the [Ca^<2+>] i-f … More orce relation curve to the left. A deletion mutant containing the N-terminal 78 amino acids of MLCPsr retained the full action, compared with the full-length MLCPsr, while the deletion of this region completely abolished its effect. The process of relaxation was also delayed by the fragment containing the N-terminal 78 amino acids. These results indicated that MLCPsr increases the Ca^<2+> sensitivity of the contractile apparatus while the N-terminal 78 amino acids are responsible for this effect in vascular smooth muscle.
2. The effects of the NH2-terminal fragments of the large regulatory subunit of myosin light chain phosphatase (MLCPlr) on the Ca^<2+>-induced contraction of smooth muscle were investigated in the Triton X-100-permeabilized porcine renal artery. MLCPlr 1-374, MLCPlr 304-511 and MLCPlr 297-374 induced leftward shifts of the [Ca^<2+>] i-force relationship. Deletion of residues 304-374 from the most potent construct, MLCPlr 1-374, abolished the Ca^<2+>-sensitizing effect. M1301-374 slowed the rate of relaxation in Ca^<2+>-free medium. The levels of myosin light chain phosphorylation (22.4%) and force (34.5%) obtained with 300 nM Ca^<2+> were increased by 3 microMMLCPlr 1-374 to 35.7 and 92.2%, respectively.These results indicated that the NH2-terminal MLCPlr fragments containing residues 304-374 inhibited myosin phosphatase, increased myosin light chain phosphorylation and increased the Ca^<2+> sensitivity of the contractile apparatus in permeabilized porcine renal artery.
3. We have isolated cDNAs for heart-specific small regulatory subunit of myosin light chain phosphatase (HS-MLCPsr) that were transcribed from a heart-specific promoter in intron 13 of the MYPT2 gene. Functional analysis in permeabilized rat cardiac myocytes and porcine renal artery revealed that HS-MLCPsr enhanced Ca^<2+>-induced contraction, and the main functional domain was mapped in the N-terminal half of HS-MLCPsr. In addition, HS-MLCPsr bound to C-terminal one-third of MYPT1, and the binding domain was also mapped in the N-terminal half of HS-MLCPsr. These results indicated that HS-MLCPsr plays a role in regulation of the cardiac muscle contraction via binding to MYPT1. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (78 results)

All Other

All Publications (78 results)

  • [Publications] Zhou YB: "The exogenously added small subunit of smooth muscle myosin phosphatase increases the Ca^<2+> sensitivity of the contractileapparatus in the permeabilizedporcine renal artery."Biochem Biophys Res Commun. 254. 158-163 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano M: "Expression, subcellular localization, and cloning of the 130-kDa regulatory subunit of myosin phosphatase in porcineaortic endothelial cells."Biochem Biophys Res Commun. 254. 490-496 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yasutsune T: "Vasorelaxation and inhibition of the voltage-operated Ca^<2+> channelsby FK506 in the porcine coronary artery."Br J Pharmacol. 126. 717-729 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhou YB: "NH_2-terminal fragments of 130 kDa subunit of myosin phosphatase increase Ca^<2+> sensitivity of porcine renal artery."J Physiol. 516. 55-65 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawasaki J: "Troglitazone inhibits the capacitative Ca^<2+> entry in endothelial cells"Eur J Pharmacol. 373. 111-120 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ihara E: "Thapsigargin-induce dendothelium-dependen triphasic regulation of vascular tone in the porcine renal artery."Br J Pharmacol. 128. 689-699 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ushio-Fukai M: "Changes in the cytosolic Ca^<2+> concentration and Ca^<2+>-sensitivity of the contractile apparatus during angiotensin II-induceddesensitization in the rabbit femoral artery."Br J Pharmacol. 129. 425-436 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ushio-Fukai M: "The mechanism of the decrease in cytosolic Ca^<2+> concentrations induced by angiotensin II in the high K^+-depolarized rabbit femoral artery."Br J Pharmacol. 129. 437-447 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizuno O: "Proteolysis and phosphorylation-mediated regulation of thrombin receptor activity in in situ endothelial cells."Eur J Pharmacol. 389. 13-23 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ihara E: "The mechanism of bradykinin-in duceden dothelium-dependent contraction and relaxation in the porcine interlobar renal artery."Br J Pharmacol. 129. 943-952 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizuno O: "Stimulus-specific alteration of the relationship between cytosolic Ca^<2+> transients and nitric oxide production in endothelial cells ex vivo."Br J Pharmacol. 130. 1140-1146 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawasaki J: "Dissociation between the Ca^<2+> signal and tube formation induced by vascular endothelial growth factor in bovine aortic endothelial cells."Eur J Pharmacol. 398. 19-29 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zeng Y: "Minimal requirement for nuclear localization of p27^<kip1>, a cyclin-dependentkinase inhibitor."Biochem Biophys Res Commun. 274. 37-42 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shintani Y: "Mechanisms underlying the neurokinin A-inducedcontraction of the pregnantrat myometrium."Br J Pharmacol. 130. 1165-1173 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroiwa-Matsumoto M: "Mechanisms of the thapsigargin-induced Ca^<2+> entry in in situ endothelial cells of the porcine aortic valve and the endothelium-dependentrelaxation in the porcine coronary artery."Br J Pharmacol. 131. 115-123 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K: "Mitogen-induced up-regulation of non-smooth muscle isoform of α-tropomyosin in rat aortic smooth muscle cells."Eur J Pharmacol. 406. 209-218 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ieiri S: "Mechanism of vasorelaxation induced by a novel types of Ca^<2+> channel blocker SR33805 in the porcine coronary artery."Br J Pharmacol. 131. 1592-1596 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shintani Y: "Enhanced contractile response to thrombin in the pregnant rat myometrium."Br J Pharmacol. 131. 1619-1628 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Derkach DN: "Thrombin causes endothelium-dependent biphasic regulation of vascular tone in the porcine renal interlobar artery."Br J Pharmacol. 131. 1635-1642 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshima J: "Differential effects of progesterone and 17 β-estradiol on the Ca^<2+> entry induced by thapsigargin and endothelin-1 in in situ endothelial cells."Biochem Biophys Acta. 1499. 109-12 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K: "Cloning and functional expression of a degradation-resistant novel isoform of p27^<Kip1>."Biochem J. 353. 51-57 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Setoguchi H: "Leukotriene C_4 enhances the contraction of porcine tracheal smooth muscle through the activation of Y-27632, a rho kinase inhibitor, sensitive pathway."Br J Pharmacol. 132. 111-118 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arimura T: "Identification, characterization and functional analysis of heart-specific myosin light chain phosphatase small subunit."J Biol Chem. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakamura K: "Hydroxy fasudil, an active metabolite of fasudil hydrochloride, relaxes the rabbit basilar artery by disinhibition of myosin light chain phosphatase."J Cereb Blood Flow Metab. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhou YB, Nishimura J, Hirano K, Kanaide H: "The exogenously added small subunit of smooth muscle myosin phosphatase increases the Ca^<2+> sensitivity of the contractile apparatus in the permeabilized porcine renal artery."Biochem Biophys Res Commun. 254. 158-163 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano M, Niiro N, Hirano K, Nishimura J, Hartshorne DJ, Kanaide H: "Expression, subcellular localization, and cloning of the 130-kDa regulatory subunit of myosin phosphatase in porcine aortic endothelial cells."Biochem Biophys Res Commun. 254. 490-496 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yasutsune T, Kawakami N, Hirano K, Nishimura J, Yasui H, Kitamura K, Kanaide H: "Vasorelaxation and inhibition of the voltage-operated Ca^<2+> channels by FK506 in the porcine coronary artery."Br J Pharmacol. 126. 717-729 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhou YB, Hirano K, Sakihara C, Nishimura J, Kanaide H: "NH_2-terminal fragments of 130 kDa subunit of myosln phosphatase increase Ca^<2+> sensitivity of porcine renal artery."J Physiol. 516. 55-65 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawasaki J, Hirano K, Hirano M, Nishimura J, Fujishima M, Kanaide H: "Troglitazone inhibits the capacitative Ca^<2+> entry in endothelial cells."Eur J Pharmacol. 373. 111-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ihara E, Hirano K, Nishimura J, Nawata H, Kanaide H: "Thapsigargin-induced endothelium-dependen triphasic regulation of vascular tone in the porcine renal artery."Br J Pharmacol. 128. 689-699 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ushio-Fukai M, Yamamoto H,Toyofuku K, Nishimura J, Hirano K, Kanaide H: "Changes in the cytosolic Ca^<2+> concentration and Ca^<2+>-sensitivity of the contractile apparatus during angiotensin II-induced desensitization in the rabbit femoral artery."Br J Pharmacol. 129. 425-436 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ushio-Fukai M, Yamamoto H, Nishimura J, Hirano K, Kanaide H: "The mechanism of the decrease in cytosolic Ca^<2+> concentration sinduced by angiotensin II in the high K^+-depolarized rabbit femoral artery."Br J Pharmacol. 129. 437-447 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizuno O, Hirano K, Nishimura J, Kubo C, Kanaide H: "Proteolysis and phosphorylation-mediated regulation of thrombin receptor activity in in situ endothelial cells."Eur J Pharmacol. 389. 13-23 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ihara E, Hirano K, Derkach DN, Nishimura J, Nawata H, Kanaide H: "The mechanism of bradykinin-induced endothelium-dependent contraction and relaxation in the porcine interlobar renal artery."Br J Pharmacol. 129. 943-952 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizuno O, Kobayashi S, Hirano K, Nishimura J, Kubo C, Kanaide H: "Stimulus-specific alteration of the relationship between cytosolic Ca^<2+> transients and nitric oxide production in endothelial cells ex vivo."Br J Pharmacol. 130. 1140-1146 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawasaki J, Hirano K, Hirano M, Nishimura J, Nakatsuka A, Fujishima M, Kanaide H: "Dissociation between the Ca^<2+> signal and tube formation induced by vascular endothelial growth factor in bovine aortic endothelial cells."Eur J Pharmacol. 398. 19-29 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zeng Y, Hirano K, Hirano M, Nishimura J, Kanaide H: "Minimal requirement for nuclear localization of p27^<Kip1>, a cyclin-dependentkinase inhibitor."Biochem Biophys Res Commun. 274. 37-42 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shintani Y, Nishimura J, Niiro N, Hirano K, Nakano H,Kanaide H: "Mechanisms under lying the neurokinin A-induced contraction of the pregnant rat myometrium."Br J Pharmacol. 130. 1165-1173 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuroiwa-Matsumoto M, Hirano K, Ahmed A, Kawasaki J, Nishimura J, Kanaide H: "Mechanisms of the thapsigargin-induced Ca^<2+> entry in in situ endothelial cells of the porcine aortic valve and the endothelium-dependen relaxation in the porcine coronary artery."Br J Pharmacol. 131. 115-123 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K, Hirano M, Eto W, Nishimura J, Kanaide H: "Mitogen-induced up-regulation of non-smooth muscle isoform of α-tropomyosin in rat aortic smooth muscle cells."Eur J Pharmacol. 406. 209-218 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ieiri S,Hirano K, Nishimura J, Suita S, Kanaide H: "Mechanism of vasorelaxation induced by a novel types of Ca^<2+> channelblocker SR33805 in the porcine coronary artery."Br J Pharmacol. 131. 1592-1596 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shintani Y, Hirano K, Nishimura J, Nakano H, Kanaide H: "Enhanced contractile response to thrombin in the pregnant rat myometrium."Br J Pharmacol. 131. 1619-1628 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Derkach DN, Ihara E, Hirano K, Nishimura J, Takahashi S, Kanaide H: "Thrombin causes endothelium-dependent biphasic regulation of vascular tone in the porcine renalinterlobar artery."Br J Pharmacol. 131. 1635-1642 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshima J, Hirano K, Nishimura J, Nakano H, Kanaide H: "Differential effects of progesterone and 17 β-estradiol on the Ca^<2+> entry induced by thapsigargin and endothelin-1 in in situ endothelial cells."Biochem Biophys Acta. 1499. 109-121 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirano K, Hirano M, Ying Zeng, Nishimura J, Kanaide H: "Cloning and functional expression of a degradation-resistant novel isoform of p27^<Kip1>."Biochem J. 353. 51-57 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Setoguchi H, Nishimura J, Hirano K, Takahashi, Kanaide H: "Leukotriene C_4 enhances the contraction of porcine tracheal smooth muscle through the activation of Y-27632, a rho kinase inhibitor, sensitive pathway."Br J Pharmacol. 132. 111-118 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arimura T, Suematsu N, Zhou Y, Nishimura J, Satoh S, Takeshita A, Kanaide H, Kimura A: "Identification, characterization and functional analysis of heart-specific myosin light chain phosphatase small subunit."J Biol Chem. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakamura K, Nishimura J, Hirano K, Ibayashi S.Fujishima M, Kanaide H: "Hydroxyfasudil, an active metabolite of fasudil hydrochloride, relaxes the rabbit basilar artery by disinhibition of myosin light chain phosphatase."J Cereb Blood Flow Metab. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zhou YB: "The exogenously added small subunit of smooth muscle myosln phosphatase increases the Ca^<2+> sensitivity of the contractileapparatus in the permeabilizedporcine renal artery."Biochem Biophys Res Commun. 254. 158-163 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano M: "Expression, subcellular localization, and cloning of the 130-kDa regulatory subunit of myosin phosphatase in porcine aortic endothelial cells."Biochem Biophys Res Commun. 254. 490-496 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yasutsune T: "Vasorelaxationandinhibition of the voltage-operated Ca^<2+> channelsby FK506 in the porcine coronary artery."Br J Pharmacol. 126. 717-729 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zhou YB: "NH_2-terminal fragments of 130 kDa subunit of myosin phosphatase increase Ca^<2+> sensitivity of porcine renal artery."J Physiol. 516. 55-65 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawasaki J: "Troglitazone inhibits the capacitative Ca^<2+> entry in endothelial cells."Eur J Pharmacol. 373. 111-120 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ihara E: "Thapsigaragin-inducedendothelium-dependentriphasic regulation of vascular tone in the porcine renal artery."Br J Pharmacol. 128. 689-699 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ushio-Fukai M: "Changesin the cytosolic Ca^<2+> concentrationand Ca^<2+>-sensitivity of the contractile apparatus during angiotensin II-induceddesensitization in the rabbit femoral artery."Br J Pharmacol. 129. 425-436 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ushio-Fukai M: "The mechanism of the decrease in cytosolic Ca^<2+> concentrations induced by angiotensin II in the high K^+-depolarizedrabbit femoral artery."Br J Pharmacol. 129. 437-447 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mizuno O: "Proteolysis and phosphorylation-mediated regulation of thrombin receptor activity in in situ endothelial cells."Eur J Pharmacol. 389. 13-23 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ihara E: "The mechanism of bradykinin-inducedendothelium-dependent contraction and relaxation in the porcine interlobar renal artery."Br J Pharmacol. 129. 943-952 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mizuno O: "Stimulus-specific alteration of the relationship between cytosolic Ca^<2+> transients and nitric oxide production in endothelial cells ex vivo."Br J Pharmacol. 130. 1140-1146 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawasaki J: "Dissociation between the Ca^<2+> signal and tube formation induced by vascular endothelial growth factor in bovine aortic endothelial cells."Eur J Pharmacol. 398. 19-29 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zeng Y: "Minimal requirementfor nuclear localization of p27^<Kip1>, a cyclin-dependentkinase inhibitor."Biochem Biophys Res Commun. 274. 37-42 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shintani Y: "Mechanisms underlying the neurokininA-inducedcontraction of the pregnantrat myometrium."Br J Pharmacol. 130. 1165-1173 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kuroiwa-Matsumoto M: "Mechanisms of the thapsigargin-induced Ca^<2+> entry in in situ endothelial cells of the porcine aortic valve and the endothelium-dependentrelaxation in the porcine coronary artery."Br J Pharmacol. 131. 115-123 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano K: "Mitogen-induced up-regulation of non-smooth muscle isoform of α-tropomyosin in rat aortic smooth muscle cells."Eur J Pharmacol. 406. 209-218 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ieiri S: "Mechanism of vasorelaxation inducedby a novel types of Ca^<2+> channel blocker SR33805 in the porcine coronary artery."Br J Pharmacol. 131. 1592-1596 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shintani Y: "Enhanced contractile response to thrombin in the pregnant rat myometrium."Br J Pharmacol. 131. 1619-1628 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Derkach DN: "Thrombin causes endothelium-dependent biphasic regulation of vascular tone in the porcine renal interlobar artery."Br J Pharmacol. 131. 1635-1642 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toshima J: "Differential effects of progesterone and 17 β-estradiol on the Ca^<2+> entry induced by thapsigargin and endothelin-1 in in situ endothelial cells."Biochem Biophys Acta. 1499. 109-12 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hirano K: "Cloning and functional expression of a degradation-resistant novel isoform of p27^<Kip1>."Biochem J. 353. 51-57 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Setoguchi H: "Leukotriene C_4 enhances the contraction of porcine tracheal smooth muscle through the activation of Y-27632,a rho kinase inhibitor, sensitive pathway."Br J Pharmacol. 132. 111-118 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Arimura T: "Identification, characterization and functional analysis of heart-specific myosin light chain phosphatase small subunit."J Biol Chem. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakamura K: "Hydroxyfasudil, an active metabolite of fasudil hydrochloride, relaxes the rabbit basilar artery by disinhibition of myosin light chain phosphatase."J Cereb Blood Flow Metab. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Ihara E,Hirano K,Nishimura J,et al.: "Thapsigargin-inducedendothelium-dependenttriphasic regulation of vascular tone in the porcine renal artery"Br J Pharmacol. 128. 689-699 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kawasaki J,Hirano K,Hirano M,Nishimura J,et al.: "Troglitazone inhibits the capacitative Ca^<2+> entry in endothelial cells"Eur J Pharmacol. 373. 111-120 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhou YB,Hirano K,Sakihara C,Nishimura J,et al.: "NH_2-terminal fragments of 130 kDa subunit of myosin phosphatase increases Ca^<2+> sensitivity of the porcine renal artery"J Physiol(London). 516. 55-65 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasutsune T,Kawakami N,Hirano K,Nishimura J,et al.: "Vasorelaxation and inhibition of the voltage-operated Ca^<2+> channels by FK506 in porcine"Br J Pharmacol. 126. 717-729 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhou YB,Nishimura J,Hirano K,Kanaide H: "The exogenously added small subunit of smooth muscle myosin phosphatase increases the Ca^<2+> sensitivity of the contractile apparatus in the permeabilized porcine renal artery"Biochem Biophys Res Commun. 254. 158-163 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirano M,Niiro N,Hirano K,Nishimura J,et al.: "Expression, subcellular localization and cloning of the 130 kDa regulatory subunit of myosin phosphatase in porcine aortic endothelial cells"Biochem Biophys Res Commun. 254. 490-496 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi