| Project/Area Number |
11839007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
KERA Yoshio Nagaoka University of Technology, Department of Environmental and Civil Engineering, Associate Professor, 工学部, 助教授 (00137168)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | endocrine disruption / biomarker / Koi carp (Cyprinus carpio) / vitellogenin / Shinano River / Sakata / Toyanogata / effluent from sewage treatment plant |
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| Research Abstract |
Vitellogenin (Vtg) is a female specific plasma protein and the major precursor of egg yolk protein in oviparous vertebrates. The protein can serve as a useful biomarker for environmental endocrine disruption. The present study was carried out to assess environmental endocrine disruption with plasma Vtg concentration in Koi carp (Cyprinus carpio) of rivers and wetland sites.
We purified Koi carp Vtg to homogeneity, raised specific polyclonal antiserum to the purified Koi carp Vtg and established a single radial immunodiffusion assay and a homologous enzyme-linked immunosorbent assay (ELISA) to measure plasma Vtg concentration.
To assess environmental endocrine disruption, wild Koi carp were collected in the wetland site Sakata and Toyanogata and the Shinano River. Two of 24 males captured in the Shinano River in the Ojiya City area and 5 of 15 males captured in the Shinano River in Niigata City area possessed slightly higher plasma Vtg concentrations (>1 μg/ml).
Plasma Vtg levels in juvenile male carp, which were exposed to effluent from sewage treatment plant for 10-14 days, were higher than those in the unexposed male fish, suggesting that the effluent contained estrogenic compounds at a biological active level. The analyses of the effluent with GC/MS revealed that estradiol- 17β in the effluent is one of the main compounds to increase plasma Vtg level in Koi carp.
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