| Project/Area Number |
11839008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | Gifu University |
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Principal Investigator |
TSUBOTA Toshio Gifu University Faculty of Agriculture, 農学部, 助教授 (10207441)
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| Co-Investigator(Kenkyū-buntansha) |
MURASE Tetsuma Gifu University, Faculty of Agriculture, Assistant professor, 農学部, 助手 (30303514)
KITA Isao Gifu University, Faculty of Agriculture, Professor, 農学部, 教授 (60021705)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Endocrine disruptor / Environmental Hormone / Wild animal / Reproductive disorder / Steroidogenesis |
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| Research Abstract |
1) Detection of Reproductive Disorder Caused by Endocrine Disruptors in Wild Animals
Organ samples were obtained from dead bodies of wild raccoon dogs, bears, mice and raptors. Dead bodies from captive fur seals, sea lions and dolphins were also used for the samples. Accumulated concentrations of endocrine disruptors (PCB, HCH, DDT and its congeners, butyltins and agricultural chemicals) were measured using the liver and adipose tissue of the samples. Histological specimens of the reproductive tracts were employed for the pathological examination. A case of testicular cancer from a raccoon dog was recognized by the examination.
2) Mechanism of Reproductive Disorder Caused by Endocrine Disruptors
Complimentary DNA made from mRNA of three kinds of steroidogenic enzymes, 3βHSD, P450c17 and P450arom, was sequenced for the study on the expression of these enzymes in the gonads. Quantity and quality of these enzyme expression will be examined by the method of Northern blotting and in situ hybridization, respectively. Some disorder of steroidogenesis in the gonads by the endocrine disruptors will be studied furthermore. The disorder of oval development and fetal differentiation are being studies still.
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