| Project/Area Number |
11839020
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | OITA MEDICAL UNIVERSITY |
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Principal Investigator |
SAKAI Kumiko FACULTY OF MEDICINE, OITA MEDICAL UNIVERSITY, Education Assistant, 医学部, 教務員 (60225753)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Satoshi FACULTY OF ENGINEERING GIFU UNIVERSITY Professor, 工学部, 教授 (50158440)
EGASHIRA Toru FACULTY OF MEDICINE, OITA MEDICAL UNIVERSITY, Associate Professor, 医学部, 助教授 (50053947)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | endocrine disruptors / styrenes / cerebral nervous system / monoamine oxidase / serotonin metabolism / monkey |
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| Research Abstract |
Ultrapure water boiled at 100℃ for 24hr with commercial styrene polymers was fractionated by C18 reversed phase HPLC to obtain a single component which inhibited the serotonine degradation activity of MAOA(monoamine oxidase A). The inhibition fraction eluted at acetonitrile concentration of 30% was examined. We reported that a major component of the MAOA inhibitory fraction was low molecular weight chemical compound with a benzene ring by FTIR and Raman spectroscopy. Further investigation by C^<13> and H^1 NMR revealed that the chemical compound was a benzene ring with a quaternary carbon molecule and re-investigation by FTIR suggested that benzene ring had one substituent. This compound was examined by means of TOFMS, GCMS and LCMS to obtain precise molecular mass and these results suggested that numbers of benzene ring was not one, but two or three.
On the basis of the results above, we investigated whether the commercial candidates for MAOA inhibitor (benzoyl peroxide, sodium benzoate, benzoic acid, phthalic acid, benzoin and benzoic anhydride) would inhibit MAOA activity. Any compound did not inhibit MAOA activity at the concentration of 1 μg/ml. Extracted inhibition fraction from polystyrene with ultrapure water strongly inhibited the activity of MAOA but not the activity of MAOB, which was a MAOA isozyme, in monkey brain mitochondria. In rat brain mitochondria, both MAOA and MAOB were not inhibited by the fraction. Furthermore, we examined effects of MAOA inhibition fraction on acetylcholinesterase in monkey and rat brain no inhibition were observed in each tissue.
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