Evaluation of the effects of endocrine disruptors in synthetic compounds on several biological organisms.

• Project/Area Number: 11839026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Institution: NIHON UNIVERSITY
• Principal Investigator: KIMURA Junpei College of Bioresource Sciences, Nihon University, Associate Professor., 生物資源科学部・獣医学科, 助教授 (30177919)
• Co-Investigator(Kenkyū-buntansha): ASAHINA Kiyoshi College of Bioresource Sciences, Nihon University, Professor., 生物資源科学部, 教授 (10147671) ; INOUE Tadashi College of Bioresource Sciences, Nihon University, Professor., 生物資源科学部, 教授 (90124213) ; KATASE Takao College of Bioresource Sciences, Nihon University, Professor., 生物資源科学部, 教授 (70070696) ; MORITOMO Tadaaki College of Bioresource Sciences, Nihon University, Assistant, 生物資源科学部, 講師 (20239677)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥2,400,000 (Direct Cost: ¥2,400,000); Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: endocrine disruptor / smear test / plastic / nonylphenol / estrogenic activity / フタル酸エステル / ビスフェノールA / 内分泌撹乱物質 / 膣前庭 / 組換え酵母検出系 / プラスチック製品 / エストロゲン / コルチゾル

Research Abstract

The estrogenic activity of thirty seven endocrine disruptors including byproducts from plastic gloves and food wraps, and chemicals related to nonylphenol, medical drugs and phytoestrogens, were measured by in vitro and in vivo tests. There was a good correlation between the results obtained from two in vitro tests which included a recombinant yeast test and a cultured mammary cancer cell test. Eighteen chemicals were subjected to an in vivo rat vaginal smear test. Those of the chemicals subjected, eight showed a positive correlation between results obtained from the present study and the results from Dodds's results (1938). From these correlation, the evaluation methods of plastic compounds, medical drugs and phytoestrogens in food safety and living environment were discussed.

Research Products

• [Publications] 片瀬隆雄: "内分泌かく乱物質研究の最前線"季刊化学総説. 50. (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] J.Kimura et al.: "The Application of a Three-dimensional Internal Structure Microscopy (3D-ism) in the Observation of Mare Ovary"Anatomia Histologia Embryologia. (In press). (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] J.Kimura et al.: "Immunohistochemical Localization of Inhibin and Steroidogenic Enzymes in the Ovary of Common Tree Shrew and Northern Smooth-tailed Tree Shrew"Anatomia Histologia Embryologia. 29. 267-271 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] J.Kimura et al.: "The Application of a Three-dimensional Internal structure Microscopy (3D-ism) in the Observation of Mare Ovary"Anatomia Histologia Embryologia. (in press). (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] J.Kimura et al.: "Immunohistochemical Localization of Inhibin and Steroidogenic Enzymes in the Ovary of Common Tree Shrew and Northern Smooth-tailed Tree Shrew."Anatomia Histologia Embryologia. 29. 267-271 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 片瀬隆雄: "内分泌かく乱物質研究の最前線"季刊化学総説. 50. (2001)
• [Publications] J.Kimura et al.: "The Application of a Three-dimensional Internal Structure Microscopy (3D-ism) in the Observation of Mare Ovary"Anatomia Histologia Embryologia. In press. (2001)
• [Publications] J.Kimura et al.: "Immunohistochemical Localization of Inhibin and Steroidogenic Enzymes in the Ovary of Common Tree Shrew and Northern Smooth-tailed Tree Shrew"Anatomia Histologia Embryologia. 29. 267-271 (2000)