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ヒト粘膜下層を用いた浸潤能の測定系の開発

Research Project

Project/Area Number 11877208
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionGunma University

Principal Investigator

桑野 博行  群馬大学, 医学部, 教授 (90186560)

Co-Investigator(Kenkyū-buntansha) 志村 龍男  群馬大学, 医学部, 助手 (00282393)
浅尾 高行  群馬大学, 医学部, 助教授 (40212469)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsヒト胃粘膜下層 / 癌細胞 / 組織培養 / 浸潤能アッセイ / 細胞培養
Research Abstract

ヒト胃癌手術時の摘出標本から正常粘膜下層を採取し、トランスウェルチャウバーのmicroporeフィルターの代わりに、この粘膜下層を接着させ浸潤能をアッセイする系を確立した。
上記の系に胃癌細胞株であるMKN45、MKN74およびKATOIIIを用いて、各々の浸潤能を測定したところ、MNK45とKATOIIIは培養3日目に粘膜下層内へ浸潤していることが確認された。
現在、浸潤能に関与すると考えられる遺伝子を導入した細胞株を樹立し、上記系でのアッセイを試みている。

Report

(3 results)
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kuwano H, et al.: "Pathogenesis of Esophageal Squamous Cell Carcinoma with Lymphoid Stroma"Hepato-Gastroenterology. 48. 458-461 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuwano H, Asao T, et al.: "Tumor cells as the origin of elevated serum α 1,3fucosyltransferase in association with malignancy"Clinical & Experimental Metastasis. 18・7. 605-610 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuwano H, Asao T, Shimura T, et al.: "A novel model for invasion of cancer cells using the submucosal layer of the human stomach"Cancer Letter. 160・2. 171-176 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuwano H, Asao T, Shimura T, et al.: "Circulating soluble Fas ligand in patients with gastric carcinoma"Cancer. 89・12. 2560-2564 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuwano H, Asao T, Shimura T, et al.: "A novel rapid colorimetric assay of carcinoembryonic antigen levels in the abdominal cavity to detect peritoneal micrometastasis during gastric cancer surgery"Cancer Letter. 149. 1-5 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kuwano H, Asao T, Shimura T, et al.: "Loss of standard type of CD44 expression in invaded area as a good indicator of lymph-node metastasis in colorectal carcinoma"Diseases of the Colon and Rectum. 43・9. 1250-1255 (2000)

    • Related Report
      2001 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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