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脊髄損傷の根本的治療-中枢神経再生への挑戦

Research Project

Project/Area Number 11877240
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionKyoto University

Principal Investigator

鈴木 義久  京都大学, 医学研究科, 助教授 (30243025)

Co-Investigator(Kenkyū-buntansha) 遠藤 克昭  京都大学, 医学研究科, 助手 (30025613)
西村 善彦  京都大学, 医学研究科, 教授 (50081790)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords神経幹細胞 / 脊髄 / 再生 / 中枢神経 / 損傷 / 免疫組織化学 / アルギン酸 / 神経再生 / 末梢神経再生
Research Abstract

本年度は主に神経幹細胞を用いた中枢神経特に脊髄の再生に関して研究を行った。損傷を加えた脊髄に神経幹細胞を直接損傷部位に移植する場合、脳脊髄液を介して移植する場合等を研究した。まず、脊髄にニューヨーク大学式脊髄圧迫損傷器具をもちいて損傷を加え、その組織の経時的変化を観察し、神経幹細胞を移植するための基礎実験を行った。神経幹細胞は胎生16日頃のラット海馬組織より採取し、浮遊培養法によりNeurosphereを得た。これを、圧迫損傷を加えた脊髄に移植した。直接損傷部位に移植したもの、第4脳室に幹細胞を注入し脳脊髄液経由で投与したものを検討した。また、脊髄に損傷を加えた直後に移植したもの、損傷後3週間経過した後移植したものも検討した。損傷された脊髄は3日目頃よりマクロファージ等の炎症細胞が侵入し、約2週間で損傷が完成し、脊髄組織内に空洞形成されることがわかった。移植した神経幹細胞は宿主の脊髄組織に、いずれも良く生着し増殖しているものと思われた。損傷部位では、いずれの移植法でも一部損傷脊髄内に侵入し、宿主脊髄細胞と相互作用をしていると思われた。免疫組織化学染色により、宿主内に侵入した神経幹細胞は、主に星状膠細胞に分化している姿が得られた。また、数は少ないが、ニューロンに分化していると思われる細胞も見られた。また、希突起膠細胞に分化している細胞はほとんど見られなかった。損傷により形成された空洞を埋めるほどの再生は得られていないが、今後の研究に期待がもてる結果が得られた。

Report

(3 results)
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Wu, S.F.: "Facial nerve repair using alginate sponge without suturing"Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki, Y: "Electrophysiological and J-HRP-tracing studies of nerve regeneration through alginate filled gap in adult rat spinal cord"Neuroscience Letters. 318. 121-124 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wu, S.F.: "New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat"Neuroscience Letters. 318. 81-84 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wu, S.F.: "Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord"Neuroscience Letters. 312. 173-176 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wu, S.F.: "Sciatic nerve regeneration through alginate with tubulation or non-tubulation repair in cat"Journal of Neurotrauma. 18. 329-338 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ide C.: "Grafting of choroid plexus ependymal cells promotes the growth of regenerating axons in the dorsal funiculus of rat spinal cord"Experimental Neurology. 167. 242-251 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kataoka, K.: "Alginate, a bioresorbable material derived from brown seaweed, enhances elongation of amputated axons of spinal cord in infant rats"Journal of Biomedical Materials Research. 54. 373-384 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki, Y: "Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo"Journal of Biomedical Materials Research. 50. 405-409 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki K: "Regeneration of transected spinal cord in young adult rats using freeze-dried alginate gal."Neuroreport. 10. 2891-2894 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki K: "Reconstruction of rat peripheral nerve gap without sutures using freeze-dried alginate,"Journal of Biomedical Materials Research. 49. 582-533 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kataoka K: "Alginate, a bioresorbable material derived from brown seaweed, enhances elongation of amputated axons of spinal cord in infant rats."Journal of Biomedical Materials Research. 54. 373-384 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitahara,A.K.: "Facial nerve repair using collagen nerve conduit"Scandinavian Journal of Plastic and Reconstru.Surg.and Hand Surg.. 33. 187-193 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,Y.: "In vivo evaluation of a novel alginate dressing"J.of Biomed.Materials Res.(Applied Biomaterial). 48. 522-527 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,Y.: "Peripheral nerve regeneration across 50mm gap repaired with a novel nerve guide composed of freeze-dried alginate gel"Neuroscience Letters. 259. 75-78 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamawaki,Y.: "Eustatian tube cartilage and medial movement of lateral pharyngeal wall on phonation"Plastic and Reconstructive Surgery. 104. 350-356 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,Y.: "Regeneration of transected spinal cord in young adult rats using freeze-dried alginate gel"Neuroreport. 10. 2891-2894 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,Y.: "Reconstruction of rat peripheral nerve gap without sutures using freeze-dried alginate gel"J.of Biomed.Materials Res.. 49. 528-533 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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