エンドトキシンショックにおける新規環状ヌクレオチド : ニトロcGMPのシグナル制御

• Project/Area Number: 11F01105
• Research Category: Grant-in-Aid for JSPS Fellows
• Allocation Type: Single-year Grants
• Section: 外国
• Research Field: Pathological medical chemistry
• Research Institution: Tohoku University (2013); Kumamoto University (2011-2012)
• Principal Investigator: 赤池 孝章 東北大学, 大学院医学系研究科, 教授
• Co-Investigator(Kenkyū-buntansha): AHMED K.a. 東北大学, 大学院医学系研究科, 外国人特別研究員 ; AHMED KhandakerAhtesham 熊本大学, 大学院・生命科学研究部, 外国人特別研究員
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2013: ¥400,000 (Direct Cost: ¥400,000); Fiscal Year 2012: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: 敗血症 / シグナル伝達 / 活性酸素 / 一酸化窒素 / プロテオミクス

Research Abstract

敗血症における重篤な臨床症状として、遷延性の血圧低下とそれにともなう多臓器不全がある。しかしながら、このような症状の進展の詳細な分子機構はいまだ十分には分かっていない。8-Nitroguanosine3', 5'-cyclic monophosphate (8-nitro-cGMP)は炎症反応にともなって生成し、親電子性を有するユニークな環状ヌクレオチドで、タンパク質のシステイン残基にcGMPを付加するタンパク質S-グアニル化という翻訳後修飾をもたらす。cGMP依存性プロテインキナーゼ(プロテインキナーゼG ; PKG)は、平滑筋の弛緩を制御するリン酸化酵素である。本研究では、PKGに対するS-グアニル化反応と、それによる酵素活性の制御機構、さらには血管弛緩反応を解析した。精製酵素標品を用いてキナーゼ活性を検討したところ、8-nitro-cGMPはPKGを強力に、かつ不可逆的に活性化した。また、organ bathによる検討から、8-nitro-cGMP処理はマウス大動脈を、不可逆的にフェニレフリンに対する血管収縮反応を減弱させた。質量分析による結果から、8-nitro-cGMPはPKGの42番目ならびにcGMP結合ドメインに存在する195番目のシステイン残基をS-グアニル化していた。PKGのS-グアニル化は、リボ多糖を投与したマウスの心臓において顕著に増加していた。以上の結果より、敗血症における8-nitro-cGMPの生成は、PKGの活性化ドメインである195番目システインのS-グアニル化を介して、PKGを遷延性かつ不可逆的に活性化し、敗血症においてみられる血圧低下に関わる可能性が示唆された。

Strategy for Future Research Activity

(抄録なし)

Research Products

• [Journal Article] Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling. (2014)
  • Author(s): Ida T, Sawa T, Ihara H, Tsucr iya Y, Watanabe Y, Kumagai Y, Suematsu M, Motohashi H, Fujii S. Matsunaga T, Yamamoto M, Ono K, Devarie-Baez N O, Xian M, Fukuto J M, Akaike T.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: (印刷中) Issue: 21 Pages: 7606-7611
  • DOI: 10.1073/pnas.1321232111
  • Peer Reviewed / Open Access
• [Journal Article] Promotion of atherosclerosis by Helicobacter cinaedi infection that involves macrophage-driven proinflammatory responses. (2014)
  • Author(s): Khan S, Rahman H N A, Okamoto T, Matsunaga T, Fujiwara Y, Sawa T, Yoshitake J, Ono K, Ahmed K A, Rahaman M d M, Oyama K, Takeya M, Ida T, Kitamura Y, Fujii S, Akaike T.
  • Journal Title: Sci. Rep. Volume: (印刷中) Issue: 1 Pages: 4680-4680
  • DOI: 10.1038/srep04680
  • NAID: 120005649608
  • Peer Reviewed / Open Access
• [Journal Article] S-Guanylation proteomics for redox-based mitochondrial signaling. (2014)
  • Author(s): Rahaman MM, Sawa T, Ahtesh am AK, Khan S, Inoue H, Irie A. Fujii S, and Akaike T.
  • Journal Title: Antioxid. Redox Signal. Volume: 20 Issue: 2 Pages: 295-307
  • DOI: 10.1089/ars.2012.4606
  • Peer Reviewed / Open Access
• [Journal Article] Redox signal regulation via nNOS phosphorylation at Ser847 in PC12 cells and rat cerebellar granule neurons. (2014)
  • Author(s): Kasamats S, Watanabe Y, Sawa T, Akaike T, Ihara H.
  • Journal Title: Biochem. J. Volume: 459 Issue: 2 Pages: 251-263
  • DOI: 10.1042/bj20131262
  • Peer Reviewed
• [Journal Article] SIRT7 controls hepatic lipid metabolism by regulating the ubiquitin-proteasome pathway. (2014)
  • Author(s): Yoshizawa T, Karim Md. F, Sato Y, Senokuchi T, Miyata K, Fukuda T, Go C, Tasaki M, Uchimura K, Kadomatsu T, Tian Z, Smolka C, Sawa T, Kakeya M, Tomizawa K, Ando Y, Araki E, Akaike T, Braun T, Oike Y, Bober E, Yamagata K.
  • Journal Title: Cell Metab. Volume: 9 Issue: 4 Pages: 712-721
  • DOI: 10.1016/j.cmet.2014.03.006
  • Peer Reviewed / Open Access
• [Journal Article] Endogenous nitrated nucleotide is a key mediator of autophagy and innate defense against bacteria. (2013)
  • Author(s): Ito C, Saito Y, Nozawa T, Fujii S, Sawa T, Inoue H, Matsunaga T, Khan S, Akashi S, Hashimoto R, Aikawa C, Takahashi E, Sagara H, Komatsu M, Tanaka K, Akaike T, Nakagawa I, Arimoto H.
  • Journal Title: Mol. Cell Volume: 52 Issue: 6 Pages: 794-804
  • DOI: 10.1016/j.molcel.2013.10.024
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of redox signaling by an electrophilic cyclic nucleotide. (2013)
  • Author(s): Akaike T, Nishida M and Fujii S.
  • Journal Title: J. Biochem. Volume: 153 Issue: 2 Pages: 131-138
  • DOI: 10.1093/jb/mvs145
  • Peer Reviewed
• [Journal Article] Redox signaling by 8-nitro-cyclic guanosine monophosphate : nitric oxide- and reactive oxygen species-derived electrophilic messenger (2013)
  • Author(s): Fujii S and Akaike T.
  • Journal Title: Antioxid. Redox Signal. Volume: 19 Issue: 11 Pages: 1236-1246
  • DOI: 10.1089/ars.2012.5067
  • Peer Reviewed
• [Journal Article] Formation, signaling functions, and metabolisms of nitrated cyclic nucleotide. (2013)
  • Author(s): Sawa T, Ihara H, Ida T, Fujii S, Nishida M and Akaike T.
  • Journal Title: Nitric Oxide Volume: 34 Pages: 10-18
  • DOI: 10.1016/j.niox.2013.04.004
  • Peer Reviewed
• [Journal Article] Inhibition of H3K18 deacetylation of SIRT7 by Myb-binding protein la (Mybbpla) (2013)
  • Author(s): Karim Md F, Yoshizawa T, Sato Y, Sawa T, Tomizawa K, A kaike T and Yamagata K.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 441 Issue: 1 Pages: 157-163
  • DOI: 10.1016/j.bbrc.2013.10.020
  • Peer Reviewed
• [Journal Article] Fluorescent probes for live cell imaging of endogenous guanine nitration (2013)
  • Author(s): Saito Y, Ito C, Fujii S, Sawa T, Akaike T and Arimoto H.
  • Journal Title: Chembiochem. Volume: 14 Issue: 9 Pages: 1068-1071
  • DOI: 10.1002/cbic.201300129
  • Peer Reviewed
• [Journal Article] Nitrated cyclic GMP modulates guard cell signaling in Arabidopsis (2013)
  • Author(s): Joudoi T, Hatakeyama T, Maeda T, Akaike T, Sawa T and Iwai S.
  • Journal Title: Plant Cell Volume: 25 Issue: 2 Pages: 558-571
  • DOI: 10.1105/tpc.112.105049
  • Peer Reviewed
• [Journal Article] S-nitrosylated a-l-acid glycoprotein kills drug-resistant bacteria and aids survival in sepsis (2013)
  • Author(s): Watanabe K, Ishima Y, Akaike T, Sawa T, Kuroda T, Ogawa W, Watanabe H, Suenaga A, Kai T, Otagiri M, and Maruyama T.
  • Journal Title: FASEB J Volume: 27 Issue: 1 Pages: 391-398
  • DOI: 10.1096/fj.12-217794
  • Peer Reviewed
• [Journal Article] Nitric oxide/soluble guanylyl cyclase signaling mediates depolarization-induced protection of rat mesencephalic dopaminergic neurons from MPP+ cytotoxicity (2013)
  • Author(s): Kurauchi Y, Hisatsune A, Isohama Y, Sawa T, Akaike T and Katsuki H.
  • Journal Title: Neurosci. Volume: 231 Pages: 206-215
  • DOI: 10.1016/j.neuroscience.2012.11.044
  • Peer Reviewed
• [Journal Article] レドックスシグナル制御と酸化ストレスバイオマーカー (2013)
  • Author(s): 赤池孝章、藤井重元
  • Journal Title: 精神科救急 Volume: 16 Pages: 69-72
• [Journal Article] 活性酸素シグナルの調節機構 ROS毒性説から脱却した新たな概念 (2013)
  • Author(s): 居原秀、井田智章、赤池孝章
  • Journal Title: Fragrance Journal Volume: 41 Pages: 75-80
• [Journal Article] 概論 : 活性酸素のシグナル伝達機能 その生理機能の再発見と酸化ストレス研究の新展開 (2013)
  • Author(s): 赤池孝章
  • Journal Title: 実験医学(増刊) Volume: 30 Pages: 22-29
• [Journal Article] 細胞の酸化ストレスを量る 親電子化合物8-nitro-cGMPの定量を例に見つける、量る、可視化する! 質量分析実験ガイド (2013)
  • Author(s): 澤 智裕、居原 秀、赤池孝章
  • Journal Title: 実験医学別冊 Pages: 151-158
• [Journal Article] NOシグナリング研究の最前線 (2013)
  • Author(s): 澤 智裕、赤池孝章
  • Journal Title: Heart View Volume: 17 Pages: 88-92
• [Journal Article] 活性酸素とガス状分子のシグナルネットワーク (2013)
  • Author(s): 澤 智裕、赤池孝章
  • Journal Title: 医学のあゆみ Volume: 247 Pages: 776-780
• [Journal Article] はじめに -活性酸素と分子状酸素のバイオセンサーと生体応答- (2013)
  • Author(s): 赤池孝章、末松誠
  • Journal Title: 医学のあゆみ Volume: 247 Pages: 727-727
• [Journal Article] Regulation of redox signaling by an electrophilic cyclic nucleotide (2013)
  • Author(s): Akaike, T., et al.
  • Journal Title: J. Biochem. Volume: 153 Pages: 131-138
  • Peer Reviewed
• [Journal Article] Redox signaling by 8-nitro-cyclic guanosine monophosphate: nitric oxide- and reactive oxygen species-derived electrophilic messenger (2013)
  • Author(s): Fujii, S. and Akaike, T.
  • Journal Title: Antioxid. Redox Signal. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Nitric oxide/soluble guanylyl cyclase signaling mediates depolarization-induced protection of rat mesencephalic dopaminergic neurons from MPP+cytotoxicity (2013)
  • Author(s): Kurauchi, Y., Akaike, T., et al.
  • Journal Title: Neurosci. Volume: 231 Pages: 206-215
  • Peer Reviewed
• [Journal Article] S-nitrosylated a-1-acid glycoprotein kills drug-resistant bacteria and aids survival in sepsis (2013)
  • Author(s): Watanabe, K., Akaike, T., et al.
  • Journal Title: FASEB J. Volume: 27 Pages: 391-398
  • Peer Reviewed
• [Journal Article] S-Guanylation proteomics for redox-based mitochondrial signaling (2013)
  • Author(s): Rahaman, Md. M., Akaike, T., et al.
  • Journal Title: Antioxid. Redox Signal. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Hydrogen sulfide anion regulates redox signaling via electrophile sulfhydration (2012)
  • Author(s): Nishida, M., Akaike, T., et at.
  • Journal Title: Nature Chem. Biol. Volume: 8 Pages: 714-724
  • Peer Reviewed
• [Journal Article] Keapl degradation by autophagy for the maintenance of redox homeostasis (2012)
  • Author(s): Taguchi, K., Akaike, T., et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 13561-13566
  • Peer Reviewed
• [Journal Article] S-guanylation of human serum albumin is a unique posttranslational modification and results in a novel class of antibacterial agents (2012)
  • Author(s): Ishima, Y., Akaike, T., et al.
  • Journal Title: J. Pharm. Sci. Volume: 101 Pages: 3222-3229
  • Peer Reviewed
• [Journal Article] Anks4b, a novel target of HNF4a protein, interacts with GRP78 protein and regulates endoplasmic reticulum stress-induced apoptosis in pancreatic β-cells (2012)
  • Author(s): Sato, Y., Akaike, T., et al.
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 23236-23245
  • Peer Reviewed
• [Journal Article] HGF-mediated ihibition of oxidative stress by 8-nitro-cGMP in high glucose-treated rat mesangial cells (2012)
  • Author(s): Guoguo, S., Akaike, T., et al.
  • Journal Title: Free Radic. Res. Volume: 46 Pages: 1238-1248
  • Peer Reviewed
• [Journal Article] Nitric oxide promotes recycling of 8-nitro-cGMP, a cytoprotective mediator, into intact cGMP in cells (2012)
  • Author(s): Saito, Y., Akaike, T., et al.
  • Journal Title: Mol. BioSystems Volume: 8 Pages: 2909-2915
  • Peer Reviewed
• [Journal Article] Nitrated cGMP: a new player in guard cell signaling (2012)
  • Author(s): Joudoi, T., Akaike, T., et al.
  • Journal Title: Plant Cell Volume: 25 Pages: 558-571
  • Peer Reviewed
• [Journal Article] 硫化水素と炎症 (2012)
  • Author(s): 藤井重元、赤池孝章
  • Journal Title: 感染・炎症・免疫 Volume: 42 Pages: 235-237
• [Journal Article] 活性酸素のシグナル伝達系と分子標的 (2012)
  • Author(s): 澤 智裕、赤池孝章
  • Journal Title: 日本臨床(増刊号) Volume: 70 Pages: 273-276
• [Journal Article] 概論:活性酸素によるレドツクス恒常性制御の新たなパラダイム転換に迫る (2012)
  • Author(s): 赤池孝章
  • Journal Title: 実験医学(増刊) Volume: 30 Pages: 2690-2696
• [Journal Article] ROS・親電子リガンドとガス状分子のシグナルネットワーク (2012)
  • Author(s): 澤 智裕、赤池孝章
  • Journal Title: 実験医学(増刊) Volume: 30 Pages: 2740-2745
• [Journal Article] Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP : potential implications for ROS signalling (2012)
  • Author(s): Ahmed KA., et al
  • Journal Title: Biochem J Volume: 441 Issue: 2 Pages: 719-730
  • DOI: 10.1042/bj20111130
  • NAID: 120004875062
  • Peer Reviewed
• [Journal Article] 新・活性酸素中毒 (2012)
  • Author(s): 赤池孝章
  • Journal Title: ファルマシア Volume: 48 Pages: 1-1
  • Peer Reviewed
• [Journal Article] 基礎の基礎 (2012)
  • Author(s): 赤池孝章
  • Journal Title: 細胞工学 Volume: 31 Pages: 134-137
  • Peer Reviewed
• [Journal Article] ニトロ化環状ヌクレオチドによるタンパク質S-グアニル化を介する酸化ストレス適応応答の分子機序 (2012)
  • Author(s): 藤井重元、赤池孝章
  • Journal Title: 生化学 Volume: 84 Pages: 124-128
  • NAID: 10030346449
  • Peer Reviewed
• [Journal Article] Frontiers in nitric oxide and redox signaling (2011)
  • Author(s): Akaike T., et al
  • Journal Title: Nitric Oxide Volume: 25 Issue: 2 Pages: 57-58
  • DOI: 10.1016/j.niox.2011.04.015
  • Peer Reviewed
• [Journal Article] Midbrain dopaminergic neurons utilize nitric oxide/cyclic GMP signaling to recruit ERK that links retinoic acid receptor stimulation to up-regulation of BDNF (2011)
  • Author(s): Kurauchi Y., et al
  • Journal Title: J Neurochem Volume: 116 Issue: 3 Pages: 323-333
  • DOI: 10.1111/j.1471-4159.2010.06916.x
  • Peer Reviewed
• [Journal Article] Vascular responses to 8-nitro-cyclic GMP in non-diabetic and diabetic mice (2011)
  • Author(s): Tokutomi Y., et al
  • Journal Title: Br J Pharmabol Volume: 162 Issue: 8 Pages: 1884-1893
  • DOI: 10.1111/j.1476-5381.2011.01201.x
  • Peer Reviewed
• [Journal Article] Protein cysteine S-guanylation and electrophilic signal transduction by endogenous nitro-nucleotides (2011)
  • Author(s): Ahmed KA., et al
  • Journal Title: Amino Acids Volume: 41 Issue: 1 Pages: 123-130
  • DOI: 10.1007/s00726-010-0535-1
  • Peer Reviewed
• [Journal Article] Antioxidant Effect of a Nitrated Cyclic Nucleotide Functioning as an Endogenous Electrophile (2011)
  • Author(s): Sawa T., et al
  • Journal Title: Curr Top Med Chem Volume: 11 Pages: 1854-1860
  • Peer Reviewed
• [Journal Article] Detoxification of methylmercury by hydrogen sulfide-producing enzyme in Mammalian cells (2011)
  • Author(s): Yoshida E, Toyama T (equalcontributor), Shinkai Y, Sawa T, Akaike T, Kumagai Y
  • Journal Title: Chemical Research in Toxicology Volume: 24 Issue: 10 Pages: 1633-1635
  • DOI: 10.1021/tx200394g
  • Peer Reviewed
• [Journal Article] Cellular uptake mechanisms and responses to NO transferred from mono- and poly-S-nitrosated human serum albumin (2011)
  • Author(s): Yu Ishima., et al
  • Journal Title: Free Radical Research Volume: 45 Issue: 10 Pages: 1196-1206
  • DOI: 10.3109/10715762.2011.606814
  • Peer Reviewed
• [Journal Article] 喫煙と酸化ストレス:Oxidative stress induced by cigarette smoking (2011)
  • Author(s): 岡本竜哉, 他
  • Journal Title: 最新精神医学 Volume: 16 Pages: 431-439
• [Journal Article] 活性酸素・一酸化窒素によるニトロ化シグナルと抗炎症作用 (2011)
  • Author(s): 澤智裕, 他
  • Journal Title: 感染・炎症・免疫 Volume: 41 Pages: 12-19
• [Journal Article] Nucleotides function as endogenous chemical sensors for oxidative stress signaling (2010)
  • Author(s): Ihara H., et al
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 48 Issue: 1 Pages: 33-39
  • DOI: 10.3164/jcbn.11-003FR
  • NAID: 130004879228
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Presentation] 環境応答とチオールバイオロジー (2014)
  • Author(s): 赤池孝章
  • Organizer: 第30回高峰カンファレンス
  • Place of Presentation: 東京會館(東京都中央区)
  • Year and Date: 2014-03-07
  • Invited
• [Presentation] 酸化ストレスとオートファジー (2014)
  • Author(s): 赤池孝章
  • Organizer: 大阪大学蛋白質研究所セミナー
  • Place of Presentation: 大阪大学蛋白質研究所(大阪府吹田市)
  • Invited
• [Presentation] Regulation of 8-nitro-cGMP electrophilic signal transduction (2014)
  • Author(s): Akaike T.
  • Organizer: The Environmental Response IV
  • Place of Presentation: 東北大学片平さくらホール(宮城県仙台市)
  • Invited
• [Presentation] 酸化ストレス応答とポリチオールバイオロジー (2014)
  • Author(s): 赤池孝章
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス(神奈川県川崎市)
  • Invited
• [Presentation] 喘息を含めた呼吸器疾患と酸化ストレス (2013)
  • Author(s): 赤池孝章
  • Organizer: 第2回東北呼吸器疾患懇談会
  • Place of Presentation: AER(宮城県仙台市)
  • Year and Date: 2013-09-28
  • Invited
• [Presentation] 酸化ストレスの新しい制御因子 : 活性イオウ分子 (2013)
  • Author(s): 赤池孝章
  • Organizer: 第236回宮城県立がんセンターセミナー
  • Place of Presentation: 宮城県立がんセンター(宮城県名取市)
  • Year and Date: 2013-09-06
  • Invited
• [Presentation] 活性酸素シグナル制御とポリサルファバイオロジー (2013)
  • Author(s): 赤池孝章
  • Organizer: 東京理科大学セミナー
  • Place of Presentation: 東京理科大学生命医科学研究所(千葉県野田市)
  • Year and Date: 2013-08-09
  • Invited
• [Presentation] 活性酸素シグナル制御とポリサルファバイオロジー (2013)
  • Author(s): 赤池孝章
  • Organizer: 国立がんセンターセミナー
  • Place of Presentation: 国立がんセンター(千葉県柏市)
  • Year and Date: 2013-08-09
  • Invited
• [Presentation] 活性酸素シグナルとオートファジー : 活性酸素によるユニークな感染防御機構 (2013)
  • Author(s): 赤池孝章
  • Organizer: 大阪大学微生物病研究所アドバンスセミナー
  • Place of Presentation: 大阪大学微生物病研究所(大阪府吹田市)
  • Year and Date: 2013-06-06
  • Invited
• [Presentation] 活性イオウ分子による活性酸素シグナル制御 (2013)
  • Author(s): 赤池孝章
  • Organizer: 第22回日本Cell Death学会学術集会
  • Place of Presentation: 京都大学医学部芝蘭会舘(京都府京都市)
  • Invited
• [Presentation] 活性酸素シグナル制御とポリスルフィドミクス (2013)
  • Author(s): 赤池孝章
  • Organizer: 第86回日本生化学会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Invited
• [Presentation] 硫化水素によるレドックスシグナル制御 (2013)
  • Author(s): 赤池孝章
  • Organizer: 第12回分子予防環境医学研究会大会
  • Place of Presentation: つくばサイエンス・インフォメーションセンター(筑波)(招待講演)
• [Presentation] 活性酸素による多階層シグナル伝達機構とレドックス恒常性制御 (2013)
  • Author(s): 赤池孝章
  • Organizer: 日本薬理学会第133年会
  • Place of Presentation: パシフィコ横浜(横浜市)(招待講演)
• [Presentation] 硫化水素によるレドックスシグナル制御:新しいセカンドメッセンジャー8-SH-cGMPの発見 (2012)
  • Author(s): 赤池孝章
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神疔国際会議場(神戸市)(招待講演) )
  • Year and Date: 2012-06-30
• [Presentation] トップジャーナル七転び八起き (2012)
  • Author(s): 赤池孝章
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎)(招待講演)
  • Year and Date: 2012-03-27
• [Presentation] Nitrated cyclic nucleotide mediates endogenous electrophilic signaling for Ras-dependent senescence and autophagy (2012)
  • Author(s): Akaike, T., et al.
  • Organizer: The 33rd Naito Conference
  • Place of Presentation: ガトーキングダムサッポロ(札幌市)(招待講演)
• [Presentation] 8-SH-cGMP endogenously formed from 8-nitro-cGMP as a second messenger of hydrogen sulfide (2012)
  • Author(s): Akaike, T., et al.
  • Organizer: The 7th International Conference on the Biology, Chemistry and Therapeutic Applications ofNitric Oxide
  • Place of Presentation: Edinburgh, UK
• [Presentation] Hydrogen sulfide regulation for electrophile-mediatec ROS signaling (2012)
  • Author(s): Akaike, T
  • Organizer: Second International Conference on h2s Biology and M edicine
  • Place of Presentation: Atlanta, USA
• [Presentation] 感染防御における活性酸素シグナル制御と破綻 (2012)
  • Author(s): 赤池孝章
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島大学(徳島市)(招待講演)
• [Presentation] レドックスシグナル制御と酸化ストレスバイオマーカー (2012)
  • Author(s): 赤池孝章
  • Organizer: 第20回日本精神科救急学会学術総会
  • Place of Presentation: 奈良県新公会堂(奈良市)(招待講演)
• [Presentation] Persulfide regulation of redox signaling involving unique polysulfur biology (2012)
  • Author(s): Akaike, T
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡)(招待講演)
• [Presentation] Cellular signaling mediated by nitrated cyclic nucleotides and its regulation by hydrogen sulfide (2011)
  • Author(s): Takaaki Akaike
  • Organizer: The 23^<rd> Annual Meeting of the Korean Society for Molecular and Cellular Biology
  • Place of Presentation: COEX(ソウル韓国)(招待講演)
  • Year and Date: 2011-10-06
• [Presentation] ROS and electrophilic cellular signaling regulated by hydrogen sulfide (2011)
  • Author(s): 赤池孝章, 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] Antimicrobial cell signaling mediated by nitrated cyclic nucleotide (2011)
  • Author(s): Takaaki Akaike
  • Organizer: 第84回日本細菌学会総会
  • Place of Presentation: 札幌コンベンションセンター(札幌)(招待講演)
  • Year and Date: 2011-09-06
• [Presentation] 内因性硫化水素イオンによるROS・親電子シグナル制御 (2011)
  • Author(s): 赤池孝章
  • Organizer: 第2回Molecular Cardiovascular Conference II
  • Place of Presentation: ホテルピアノ(北海道キロロ)(招待講演)
  • Year and Date: 2011-09-02
• [Presentation] 生体内ガス分子と活性酸素による多次元シグナルネットワーク (2011)
  • Author(s): 赤池孝章
  • Organizer: 第8回Heme Oxygenase研究フォーラム
  • Place of Presentation: 京都府立医科大学・青蓮会館(京都)(招待講演)
  • Year and Date: 2011-08-26
• [Presentation] Protein S-guanylation and its unique regulation mechanisms involving cysteine metabolism (2011)
  • Author(s): Takaaki Akaike
  • Organizer: 12th International congress on amino acids, peptides and proteins
  • Place of Presentation: Beijing International Convention Center(北京)
  • Year and Date: 2011-08-01
• [Presentation] 親電子シグナルと硫化水素.九州大学生体防御医学研究所共同利用 (2011)
  • Author(s): 赤池孝章
  • Organizer: 九州大学生体防御医学研究所共同利用研究集会
  • Place of Presentation: 九州大学生体防御医学研究所会議室(福岡)(招待講演)
  • Year and Date: 2011-07-22
• [Presentation] 内因性硫化水素イオンによる親電子シグナル制御 (2011)
  • Author(s): 赤池孝章
  • Organizer: 64回日本酸化ストレス学会学術集会
  • Place of Presentation: ルスツリゾートホテル&コンベンション(北海道留寿都)(招待講演)
  • Year and Date: 2011-07-02
• [Presentation] Cellular signaling mediated by nitrated cyclic nucleotide and regulated by hydrogen sulfide. 5th International Conference on cGMP (2011)
  • Author(s): Takaaki Akaike
  • Organizer: 第5回cGMP国際会議
  • Place of Presentation: Lowengebaude, (Halle, Germany)(招待講演)
  • Year and Date: 2011-06-23
• [Presentation] 活性酸素シグナル伝達と加齢 (2011)
  • Author(s): 赤池孝章
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 国立京都国際会館(京都)(招待講演)
  • Year and Date: 2011-05-27
• [Presentation] Electrophilic cellular signaling regulated by H_2S-medaited sulfhydration (2011)
  • Author(s): Takaaki Akaike
  • Organizer: 第11回日本NO学会総会
  • Place of Presentation: 昭和薬科大学(東京都町田市)
  • Year and Date: 2011-05-13
• [Book] イラストで徹底理解するシグナル伝達キーワード事典(山本 雅、仙波憲太郎、山梨裕司 編)NOシグナリング,p 67-69 (2012)
  • Author(s): 藤井重元、赤池孝章
  • Total Pages: 3
  • Publisher: 羊土社
• [Book] Antioxidiant adaptive response of malignant glioma related to resistance to antitumor treatment. In Novel Therapeutic Concepts in Targeting Glioma (eds., Faris Farassati), p.247-262 (2011)
  • Author(s): Sawa, T., Akaike T
  • Total Pages: 15
  • Publisher: InTech (Open Access Company)
• [Book] 免疫の事典一酸化窒素nitric oxide/NO.p.39(桂義元,河本宏,小安重夫,山本一彦編) (2011)
  • Author(s): 澤智裕, 赤池孝章
  • Total Pages: 1
  • Publisher: 朝倉書店
• [Book] 免疫の事典活性酸素reactive oxygen species (ROS). p.109(桂義元,河本宏,小安重夫,山本一彦編) (2011)
  • Author(s): 澤智裕, 赤池孝章
  • Total Pages: 1
  • Publisher: 朝倉書店
• [Remarks] 東北大大学院医学系研究科環境保健医学分野
  • URL: http://www.toxicosci.med.tohoku.ac.jp/index.html
• [Remarks]
  • URL: http://kumadai-bisei.com/
• [Remarks]
  • URL: http://www.ros-signal.jp/
• [Remarks]
  • URL: http://kumadai-bisei.com/
• [Remarks]
  • URL: http://www.ros-signal.jp/