| Project/Area Number |
12045230
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
|
| Allocation Type | Single-year Grants |
|---|
| Review Section |
Science and Engineering
|
|---|
| Research Institution | Shizuoka University |
|---|
Principal Investigator |
WATANABE Naoharu Faculty of Agriculture of Shizuoka University, Professor, 農学部, 教授 (90230979)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2000: ¥6,400,000 (Direct Cost: ¥6,400,000)
|
|---|
| Keywords | flower opening / floral scent formation / rose flowers / 2-phenylethanol / biogenesis / flower induction / Pharbitis nil / KODA / 2-phehylethanol / β-glucosidase / methyltransferase / 2-phenylethanol / FIF / 香気発散 / ローフェニルエタノール / C_<13>-ノルイソプレノイド |
|---|
| Research Abstract |
1.Diurnal Floral Scent Emission form flowrs of Rosa damascena, R.'Hoh-Jun' Rhythmic cycles in the emission of 2-phenyletanol (1) are observed in Rosa damascena quatra saison and R.'Hoh-Jun', although R.damascena Mill.transiently emits 1.
2.Role of glucosidase inhibitor in 2-phenylethanol emission During the day-time after the feeding of β-glucosidase-inhibitor commenced, the level of 2-phenylethyl β-D-glucopyranoside (2) remained fairly constant in the fed group, but decreased in feeding commenced in the control group, but the concentration was almost constant during the unfurling process in the fed group One β-glucosidase clone was obtained from the petals of Rose damascena Mill.although its function is not clarified yet.
3.Biogenesis of 1 and 2 [^2H_8]-1 and [^2H_8]-2 must be biosynthesized from [^2H_8] L-phenylalanine with a retention of the deuterium atom at α-position of [^2H_8] L-phenylalanine.We fed deuterium-labeled intermediates into R.'Hoh-Jun' flowers.A high incorporation of phenylacetaldoxime was confirmed.We have proposed a plausible biosynthetic pathway for 1 and 2.
4.Cloning of O-methyltransferases involved in the biosynthesis of floral scent compounds. New OMT, POMT, was purified from flower petals, and characterized the partial sequences by MALDI-TOFMS.We also confirmed the presence of carotenoid cleavage enzymes in rose flowers.
5.KODA, a compound involved in flower-induction of Pharbitis nil.Violet (collaborative reseearch with Dr.Yokoyama, Shiseido Co. Ltd) α-Ketol linolenic acid (KODA) was identified for the first time in seedlings of Pharbitis grown under a flower-inductive condition (16-h dark exposure), by means of LC-SIM and LC-MS/MS analyses.One of the metabolites of KODA has been characterized by LC-MS-MS and NMR.
|
