| Project/Area Number |
12052207
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | TAMAGAWA UNIVERSITY (2004-2005)
The University of Tokyo (2000-2003) |
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Principal Investigator |
HIBI Tadaaki Tamagawa U., Research Inst., Prof., 学術研究所, 教授 (50261954)
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| Co-Investigator(Kenkyū-buntansha) |
NAMBA Shigetou U.Tokyo, Grad S., Agr.Life Sci., Prof., 大学院農学生命科学研究科, 教授 (50189221)
SHIRAKO Yukio U.Tokyo, Asian Nat.Environ.Sci.Center, Prof., アジア生物資源環境研究センター, 教授 (90143023)
FUJIWARA Tohru U.Tokyo, Grad S., Agr.Life Sci., Assoc.Prof., 大学院農学生命科学研究科, 助教授 (80242163)
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| Project Period (FY) |
2000 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥92,300,000 (Direct Cost: ¥92,300,000)
Fiscal Year 2004: ¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2003: ¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 2002: ¥19,400,000 (Direct Cost: ¥19,400,000)
Fiscal Year 2001: ¥19,900,000 (Direct Cost: ¥19,900,000)
Fiscal Year 2000: ¥20,400,000 (Direct Cost: ¥20,400,000)
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| Keywords | plant virus / RNA replication / cell-to-cell movement / host protein / TMV / TVX / ASGV / SBWMV / PVX / チオレドキソンh / チオレドキシンh / 宿主因子 / TMW / CTLV / 形質転換植物 |
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| Research Abstract |
1. A tobacco translation elongation factor 1A (eEF1A) that binds to TMV RNA polymerase was demonstrated to be an essential host factor of TMV replication complex by overexpression or suppression of eEF1A gene in the infected plants. While, a tobacco ringfinger protein PHF15 that also binds to TMV RNA polymerase was indicated to be an inhibitory factor for TMV infection by the same experiments as the above.
2. A tobacco co-chaperon NtDnaJ-3 that binds to TMV movement protein (MP) and a tobacco transcription factor NtKN1 that binds to NtDnaJ-3 were both indicated to be essential for stable MP accumulation which leads the infected cell to facilitate the viral
3. To elucidate the interaction between MPs and host proteins required for viral movement, yeast two-hybrid screening using cDNA library clones from plant mRNA was performed. The partial or full-length sequences of TGBpl,-2,-3, and CP of a potexvirus (Tulip virus X, TVX), or the MP and CP of a capillovirus (Apple stem grooving virus, A
… More
SGV) were used as bait. The ankyrin-repeat domain containing protein (ARD) was identified as a TGBp2-interacting protein. In planta ARD knock-down experiments indicated that ARD promotes TVX cell-to-cell movement. By contrast, ASGV MP did not interact with ARD. These data suggest that plant viruses require different host factors for cell-to-cell movement depending on the type of MP.
4. Formation of RNA replication complexes of Soil-borne wheat mosaic virus was examined using Rep RNA1 consisting 5'-and 3'-untranslated regions and p152/p211 replicase genes as an inoculum and barley mesophyll protoplasts as host cells. Replication of Rep RNA 1 reached the maximum at 9 hours post-inoculation (hpi) while p152/p211 translation continued until 24 hpi. p152/p211 at 12 hpi were detected in soluble fractions of P1 and S15 whereas those at 24 hpi were mostly detected in an insoluble P1 fraction. These results suggest that Rep RNA 1 replicates at the early stages of infection in membrane-bound soluble RNA replication complexes and that p152/p211 complexes accumulated at the late stages of infection are insoluble and inactive in RNA replication. The molecular ratio of p152 to p211 was approximately 4 : 1. Less
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