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Structural and functional analyses of the plant host proteins associated with viral infection.

Research Project

Project/Area Number 12052207
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTAMAGAWA UNIVERSITY (2004-2005)
The University of Tokyo (2000-2003)

Principal Investigator

HIBI Tadaaki  Tamagawa U., Research Inst., Prof., 学術研究所, 教授 (50261954)

Co-Investigator(Kenkyū-buntansha) NAMBA Shigetou  U.Tokyo, Grad S., Agr.Life Sci., Prof., 大学院農学生命科学研究科, 教授 (50189221)
SHIRAKO Yukio  U.Tokyo, Asian Nat.Environ.Sci.Center, Prof., アジア生物資源環境研究センター, 教授 (90143023)
FUJIWARA Tohru  U.Tokyo, Grad S., Agr.Life Sci., Assoc.Prof., 大学院農学生命科学研究科, 助教授 (80242163)
Project Period (FY) 2000 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥92,300,000 (Direct Cost: ¥92,300,000)
Fiscal Year 2004: ¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2003: ¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 2002: ¥19,400,000 (Direct Cost: ¥19,400,000)
Fiscal Year 2001: ¥19,900,000 (Direct Cost: ¥19,900,000)
Fiscal Year 2000: ¥20,400,000 (Direct Cost: ¥20,400,000)
Keywordsplant virus / RNA replication / cell-to-cell movement / host protein / TMV / TVX / ASGV / SBWMV / PVX / チオレドキソンh / チオレドキシンh / 宿主因子 / TMW / CTLV / 形質転換植物
Research Abstract

1. A tobacco translation elongation factor 1A (eEF1A) that binds to TMV RNA polymerase was demonstrated to be an essential host factor of TMV replication complex by overexpression or suppression of eEF1A gene in the infected plants. While, a tobacco ringfinger protein PHF15 that also binds to TMV RNA polymerase was indicated to be an inhibitory factor for TMV infection by the same experiments as the above.
2. A tobacco co-chaperon NtDnaJ-3 that binds to TMV movement protein (MP) and a tobacco transcription factor NtKN1 that binds to NtDnaJ-3 were both indicated to be essential for stable MP accumulation which leads the infected cell to facilitate the viral
3. To elucidate the interaction between MPs and host proteins required for viral movement, yeast two-hybrid screening using cDNA library clones from plant mRNA was performed. The partial or full-length sequences of TGBpl,-2,-3, and CP of a potexvirus (Tulip virus X, TVX), or the MP and CP of a capillovirus (Apple stem grooving virus, A … More SGV) were used as bait. The ankyrin-repeat domain containing protein (ARD) was identified as a TGBp2-interacting protein. In planta ARD knock-down experiments indicated that ARD promotes TVX cell-to-cell movement. By contrast, ASGV MP did not interact with ARD. These data suggest that plant viruses require different host factors for cell-to-cell movement depending on the type of MP.
4. Formation of RNA replication complexes of Soil-borne wheat mosaic virus was examined using Rep RNA1 consisting 5'-and 3'-untranslated regions and p152/p211 replicase genes as an inoculum and barley mesophyll protoplasts as host cells. Replication of Rep RNA 1 reached the maximum at 9 hours post-inoculation (hpi) while p152/p211 translation continued until 24 hpi. p152/p211 at 12 hpi were detected in soluble fractions of P1 and S15 whereas those at 24 hpi were mostly detected in an insoluble P1 fraction. These results suggest that Rep RNA 1 replicates at the early stages of infection in membrane-bound soluble RNA replication complexes and that p152/p211 complexes accumulated at the late stages of infection are insoluble and inactive in RNA replication. The molecular ratio of p152 to p211 was approximately 4 : 1. Less

Report

(6 results)
  • 2005 Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report

Research Products

(40 results)

All 2005 2004 2003 Other

All Journal Article (18 results) Publications (22 results)

  • [Journal Article] A single amino acid residue of the protein encoded by ORF1 in the Potato virus X genome determines the symptoms in Nicotiana plants.2005

    • Author(s)
      Kagiwada, S, Yamaji, Y., Komatsu, K., Namba, S.et al.
    • Journal Title

      Virus Res. 110

      Pages: 177-182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stable deletions arising in the readthrough region of Soil-borne wheat mosaic virus RNA2 define the 5' limit of the functional promoter for the p19 subgenomic RNA.2005

    • Author(s)
      Yamamiya, A., Miyanishi, M., Shirako, Y.
    • Journal Title

      Arch. Virology 150

      Pages: 1871-1884

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A single amino acid residue of the protein encoded by ORF1 in the Potato virus X genome determines the symptoms in Nicotiana plants.2005

    • Author(s)
      Kagiwada, S, Yamaji, Y., Komatsu, K., Takahashi, S., Hirata, H., Suzuki, M., Ugaki, M., Namba, S.
    • Journal Title

      Virus Res. 110

      Pages: 177-182

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stable deletions arising in the readthrough region of Soil-borne wheat mosaic virus RNA2 define the 5' limit of the functional promoter for the p19 subgenomic RNA.2005

    • Author(s)
      Yamamiya, A., Miyanishi, M., Shirako, Y.
    • Journal Title

      Arch.Virology 150

      Pages: 1871-1884

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A single amino acid residue of the protein encoded by ORF1 in the Potato virus X genome determines the symptoms in Nicotiana plants.2005

    • Author(s)
      Kagiwada, S. et al.
    • Journal Title

      Virus Res. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Stable deletions arising in the readthrough region of Soil-borne wheat mosaic virus RNA2 define the 5' limit of the functional promoter for the p19 subgenomic RNA.2005

    • Author(s)
      Yamamiya, A. et al.
    • Journal Title

      Arch.Virology 150(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translational elongation factor 1A.2005

    • Author(s)
      Yamaji, Y. et al.
    • Journal Title

      Virology (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interaction between the helicase domain of Tobacco mosaic virus replicasce and a tobacco arginine decarboxylase.2004

    • Author(s)
      Shimizu, T., Yamaji, Y., Ogasawara, Y., Hamada, K., Sakurai, K., Hibi, T.
    • Journal Title

      Journal of General Plant Pathology 70

      Pages: 353-358

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction between the helicase domain of Tobacco mosaic virus replicase and a tobacco arginine decarboxylase.2004

    • Author(s)
      Shimizu, T., Yamaji, Y., Ogasawara, Y., Hamada, K., Sakurai, K., Kobayashi, T., Watanabe, T., Hibi, T.
    • Journal Title

      J.Gen.Plant Pathol. 70

      Pages: 353-358

    • NAID

      10014083301

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction between the helicase domain of Tobacco mosaic virus replicase and a tobacco arginine decarboxylase.2004

    • Author(s)
      Shimizu, T. et al.
    • Journal Title

      Journal of General Plant Pathology 70

      Pages: 353-358

    • Related Report
      2004 Annual Research Report
  • [Journal Article] RNA recombination between eucumoviruses : possible role of predicted stem-loop structures and an internal subecnomic promoter-like motif.2003

    • Author(s)
      Suzuki, M., Hibi, T., Masuta, C.
    • Journal Title

      Virology 306

      Pages: 77-86

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The optimal temperature for RNA replication in cells infected by Soil-borne wheat mosaic virus is 17゜C.2003

    • Author(s)
      Ohsato, S., Miyanishi, M., Shirako, Y.
    • Journal Title

      Journal of General Virology 84

      Pages: 995-1000

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus.2003

    • Author(s)
      Suzuki, M., Yoshida, M., Yoshinuma, T., Hibi, T.
    • Journal Title

      Journal of General Virology 84

      Pages: 1931-1939

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A single silent substitution in the Apple stem grooving virus genome causes symptom attenuation.2003

    • Author(s)
      Hirata, H., Lu, X., Yamaji Y., Namba S.et al.
    • Journal Title

      Journal of General Virology 84

      Pages: 2579-2583

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] RNA recombination between cucumoviruses : possible role of predicted stem-loop structures and an internal subgenomic promoter-like motif.2003

    • Author(s)
      Suzuki, M., Hibi, T., Masuta, C.
    • Journal Title

      Virology 306

      Pages: 77-86

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The optimal temperature for RNA replication in cells infected by Soil-borne wheat mosaic virus is 17 ℃.2003

    • Author(s)
      Ohsato, S., Miyanishi, M., Shirako, Y.
    • Journal Title

      J.Gen.Virol. 84

      Pages: 995-1000

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus.2003

    • Author(s)
      Suzuki, M., Yoshida, M., Yoshinuma, T., Hibi, T.
    • Journal Title

      J.Gen.Virol. 84

      Pages: 1931-1939

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A single silent substitution in the genome of Apple stem grooving virus causes symptom attenuation.2003

    • Author(s)
      Hirata, H., Lu, X., Yamaji Y., Kagiwada, S., Ugaki M., Namba, S.
    • Journal Title

      J.Gen.Virol. 84

      Pages: 2579-2583

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Suzuki, M., Hibi, T., Masuta, C.: "RNA recombination between cucumoviruses : possible role of predicted stem-loop structures and an internal subgenomic promoter-like motif"Virology. 306. 77-86 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki, M., Yoshida, M., Yoshinuma, T., Hibi, T: "Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus"Journal of General Virology. 84. 1931-1939 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirata, H., Lu, X., Yamaji, Ugaki M., Namba S.: "A single silent substitution in the Apple stem grooving virus genome causes symptom attenuation"Journal of General Virology. 84. 2579-2583 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shimizu, T., Yamaji, Y., Ogasawara, Y., Hamada, K., Sakurai, K., Kobayashi, T., Hibi, T.: "Interaction between the helicase domain of Tobacco mosaic virus replicase and a tobacco arginine decarboxylase"Journal of General Plant Pathology. in print. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Volkmann D, Mori T, Tirlapur UK, Konig TK., Fujiwara T.: "Unconventional Myosins of the Plant-Specific Class VIII : Endocytosis, Cytokinesis, Plasmodesmata/Pit-Fields, and Cell-to-Cell Coupling"Cell Biol.Int.. 3. 289-291 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hibi, T.: "SbCMV-like viruses"The Springer Index of Viruses. 217-221 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kagiwada, S., Yamaji, Y., Ugaki, M., Namba, S.: "The complete nucleotide sequence of Potato virus X strain OS : the first complete sequence of a Japanese isolate"Journal of General Plant Pathology. 68. 94-98 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakabayashi H., Yamaji., Ugaki, M., Namba, S: "The complete nucleotide sequence of a Japanese isolate of While clover mosaic virus strain RC"Journal of General Plant Pathology. 68. 173-176 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, M., Hibi, T., Masuta, C.: "RNA recombination between cucumoviruses : possible role of predicted stem-loop structures and an internal subgenomic promoter-like motif"Virology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suzuki, M., Yoshida, M.Yoshinuma, T., Hibi, T.: "Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus"Journal of General Virology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takemoto, Y., Hibi, T.: "Soybean chlorotic mottle virus genes I a, II, III, IV and V are essential but the gene I b product is nonessential for systemic infection"J. Gen. Virology. 82. 1481-1489 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Lu, X., Namba, S.et al.: "Random mutagenesis in a plant viral genome using a DNA repair-deficient mutator Escherichia coli strain"J. Virol. Methods. 94. 37-43 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oshima, K., Namba, S.et al.: "A Plasmid of phytoplasma encodes a unique replication protein having both plasmid-and virus-like domains : Clue to viral ancestry or result of virus/plasmid recombination?"Virology. 285. 270-277 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaji, Y.Namba, S.et al.: "Molecular evidence that a lily-infecting strain of Thlip breaking virus from Japan is a strain of Lily mottle virus"Eur. J. Plant Pathology. 146. 2309-2320 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaji, Y.Namba, S.et al.: "Complete nucleotide sequence of Tulip virus X (TVX-J) : borderline between species and strains within the genus Potexvirus"Archives of Virology. 107. 833-837 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Miyanishi, M., Shirako, Y.et al.: "Reassortment between genetically distinct Japanese and US strains of Soil-borne wheat mosaic virus : RNA1 from a Japanese strain and RNA2 from a US strain make a pseudorecombinant virus"Archives of Virology. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takemoto,Y.,Hibi,T. et al.: "The nucleotide sequence and genome organization of Japanesei iris necrotic ring virus, a new species in the genus Carmovirus."Arch.Virol.. 145. 651-657 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takemoto,Y.,Hibi,T.: "Soybean chlorotic mottle virus genes Ia,II,III,IV and V are essential but the gene Ib product is nonessential for systemic infection."J.Gen.Virology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Lu,X.,Namba,S. et al.: "Random mutagenesis in a plant viral genome using a DNA repair-deficient mutator Escherichia coli strain"J.Virol.Methods. 93(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamaji,Y.Namba,S. et al.: "Molecular evidence that a lily-infecting strain of TBV from Japan is a new potyvirus."Eur.J.Plant Pathology. 107(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujimaki,S.,Fujiwara,T. et al.: "A new method for direct introduction of chemicals into a single sieve tube of intact rice plants."Plant Cell Physiology. 41. 124-128 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishiwatari,Y.,Fujiwara,T. et al.: "In situ hybridization study of the rice phloem thioredoxin h mRNA accumulation-possible involvement in the differentiation of vascular tissues"Physiol.Plant.. 109. 90-96 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-03-31   Modified: 2018-03-28  

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