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Genetic mechanism controlling the transportation and accumulation of glutelin into protein body in rice endosperm

Research Project

Project/Area Number 12138206
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKyushu University

Principal Investigator

SATOH Hikaru  Kyushu Univ., Faculty of Agriculture, Prof., 大学院・農学研究院, 教授 (70128031)

Co-Investigator(Kenkyū-buntansha) KUMAMARU Toshihiro  Kyushu Univ., Faculty of Agriculture, Assoc. Prof., 大学院・農学研究院, 助教授 (00284555)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥38,300,000 (Direct Cost: ¥38,300,000)
Fiscal Year 2003: ¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 2002: ¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 2001: ¥12,800,000 (Direct Cost: ¥12,800,000)
KeywordsRice / Seed Storage Protein / Glutelin / Prolamin / Intracellular Transportation / Mutation / Endoplasmic Reticulum / Protein Storage Vacuole / 成分育種 / 化学変異原 / 胚乳貯蔵タンパク質 / 突然変異体 / MNU受精卵処理
Research Abstract

To elucidate the mechanism of intracellular transportation and accumulation of rice seed storage protein, 130 Iines of 57H mutant, which are characterized by the increased amount of proglutelin with 57 kD, were produced and novel 4 genes for 57H trait were identified. Based on genetic and histochemical analyses of the mutants, the function of each gene was discussed.
-Mutation for the sorting of storage proteins within endoplasmic reticulum (ER), Type1 mutation The analysis of esp2 mutation deleted PDI demonstrated that PDI was indispensable for the sorting of proglutelin precursor and prolamin within ER Iumen. glup4 and glup6 mutations also were classified into Type1 mutation, showing that at least 3 genes participate in the sorting of both proteins within ER lumen.
-Mutation for transportation from ER to protein storage vacuole (PSV), Type2 mutation In Type2 mutation, proglutelin precursor and prolamin were accumulated into separated particles within ER derived PB. Glup1, glup2, Glup5, glup7, Glup8 mutations were classified into Type2 mutation, showing that at least 5 genes participate in the transportation of proglutelin from ER lumen to PSV.
-Mutation for proteolysis within PSV. Type3 mutation The analysis of glup3 mutation demonstrated that VPE was indispensable for proteolytical cleavage of proglutelin precursor within PSV. glup3 mutation accumulated the progiutelin and the cleaved form of glutelin in PSV together, showing that at least 2 VPEs participate in proteolysis of proglutelin precursor.
These results demonstrated that the pathway of transportation and accumulation of glutelin in seed was divided into steps and that the genes regulating each step were 1) Targeting of glutelin mRNA to cis-ER, 2) Sorting of proglutelin precursor within ER lumen, Esp2 (PDI), Glup4, Glup6, 3) Transportation of proglutelin precursor into PSV, glupl, Glup2, glup5, Glup7, glup8, 4) Proteolysis of proglutelin precursor within PSV, Glup3 (VPE),

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] L.Qu et al.: "Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene"Theoretical and Applied Genetics. 107. 20-25 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Md.Jahan et al.: "Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources."Genetic Resources and Crop Evolution. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Sato et al.: "Gene mapping and immunocytochemistry of rice glup6 mutant."Rice Genetics Newsletter. 20. 42-43 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Sato et al.: "Mapping of glup4 gene for highly accumulating glutelin precursor polypeptides in rice"Rice Genetics Newsletter. 20. 43-45 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Takemoto et al.: "The rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfide isomerase"Plant Physiology. 128. 1212-1222 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] L.Qu et al.: "Inheritance of alleles for glutelin α-2 subunit genes in rice and identificalion of their corresponding cDNA"Theoretical and Applied Genetics. 105. 1099-1108 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kumamaru et al.: "High-resolution mapping of glup3 gene accumulating high amount of glutelin precursor"Rice Genetics Newsletter. 19. 62-63 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Ushijima et al.: "Mapping of esp1 gene for cystein-poor (CysP) prolamin decreased mutant in rice"Rice Genetics Newsletter. 19. 64-65 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pa Pa et al.: "Genetic diversity of seed storage prolamins, in Myanmar local rice cultivars"Rice Genetics Newsletter. 19. 65-67 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.S.Jahan et al.: "Variation of glutelin seed storage protein in Bangladesh rice cuiltivars, a primary study"Rice Genetics Newsletter. 18. 43-45 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pa Pa, et al.: "Genetic variation in seed storage protein glutelin of Myanmar local rice cultivars"Rice Genetics Newsletter. 18. 50-52 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.D.Tian, et al.: "Gene analysis of 57H mutant gene, glup6, in rice"Rice Genetics Newsletter. 18. 48-50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Satoh, H., Y.Uemura, T.Katayama S.Minakuchi T.Kumamaru, M.Ogawa: "Genetic diversity of endosperm stoage nutrients in rice."eds. 'Integration of Biodiversity and Genome Technology for Crop Improvement'. 89-92 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sadar Uddin Siddiqui, H.Satoh, T.Kumamaru: "Diversity in Pakistan rice genetic resources for endosperm storage."eds. 'K.Oono, T.Komatsuda, K.Kadowaki and D.Vaughan', Integration of Biodiversity and Genome Technology for Crop Improvement'. 169-170 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Dong D.L., H.Satoh, T.Kumamaru, Qu, L.Q., U.S.Sadar: "Protein marker of BT type hybrid and its three lines in japonica rice (Oryza sativa, L.)."Chinese J. of Rice Sci.. 15. 165-168 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jahan, M.S., T.Kumamaru, H.Satoh, A.Hamid: "Variation of glutelin seed storage protein in Bangladesh rice cultivars, a primary study."Rice Genetics Newslet.. 18. 43-45 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aung, Pa Pa, T.Kumamaru, H.Satoh: "Genetic variation in seed storage protein glutelin of Myanmar local rice cultivars."Rice Genetics Newslet.. 18. 50-52 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Aung, Pa Pa, T.Kumamaru, H.Satoh: "Variation of three important starch characters in Myanmar local rice cultivars for cooking and eating quality."Rice Genetics Newslet.. 18. 46-47 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tian H.D., T.Kumamaru, H.Satoh: "Gene analysis of 57H mutant gene, glup6, in rice."Rice Genetics Newslet.. 18. 48-50 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takemoto Y., S.J.Coughlan, T.W.Okita, H.Satoh, M.Ogawa, T.Kumamaru: "The rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfied isomerase."Plant Physiol. 128. 1212-1222 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Qu L.Q., X.L.Wei, H.Satoh, T.Kumamaru, M.Ogawa, F.Takaiwa: "Inheritance of alleles for glutelin α-2 subunit genes in rice and identification of their corresponding cDNA."Theor. Appl. Genet.. 105. 1099-1108 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Kumamaru, Y.Uemura, Y.Takemoto, M.Ogawa, H.Satoh: "High-resolution mapping of glup3 gene accumulating high amount of glutelin precursor."Rice Genetics Newsletter. 19. 62-63 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ushijima, T., H.Satoh, T.Kumamaru: "Mapping of esp1 gene for cystein-poor (CysP) prolamin decreased mutant in rice."Rice Genetics Newsletter. 19. 64-65 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pa Pa Aung, T.Kumamaru, H.Satoh: "Genetic diversity of seed storage prolamins, in Myanmar local rice cultivars."Rice Genetics Newsletter. 19. 65-67 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Qu, L.Q., X.L.Wei, H.Satoh, T.Kumamaru, M.Ogawa, F.Takaiwa: "Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene."Theor. Appl. Genet.. 107. 20-25 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Pa Pa Aung, T.Kumamaru, H.Satoh: "Diversity of glutelin basic subunit among Myanmar local rice cultivars."Rice Genetics Newsletter. 20. 36-39 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato M., D.Ishihara, H.D.Tian, Y.Takemoto, T.Kumamaru, H.S.Satoh: "Gene mapping and immunocytochemistry of rice glup6 mutant."Rice Genetics Newsletter. 20. 42-43 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Satoh, Y.Takemoto, T.Kumamaru, H.Satoh: "Mapping of glup4 gene for highly accumulating glutelin precursor polypeptides in rice."Rice Genetics Newsletter. 20. 43-45 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uemura Y., T.Kumamaru, M.Ogawa, H.Satoh: "A novel glutelin gene located on chromosome 3 in rice."Rice Genetics Newsletter. 20. 45-47 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Uemura Y., T.Kumamaru, M.Ogawa, H.Satoh: "Glu6 gene encodes a glutelin polypeptide containing α-2 acidic subunit with pI 6.55."Rice Genetics Newsletter. 20. 47-48 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Jahan Md.S., Y.Uemura, T.Kumamaru, A.Hamid, H.Satoh: "Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources."Genetic Resources and Crop Evolution (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] L.Qu et al.: "Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene"Theoretical and Applied Genetics. 107. 20-25 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Md.Jahan et al.: "Genetic variation of glutelin acidic subunit polypeptides in Bangladesh rice genetic resources."Genetic Resources and Crop Evolution. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Sato et al.: "Gene mapping and immunocytochemistry of rice glup6 mutant."Rice Genetics Newsletter. 20(印刷中).

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Sato et al.: "Mapping of glup4 gene for highly accumulating glutelin precursor polypeptides in rice"Rice Genetics Newsletter. 20(印刷中).

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Takemoto, S.J.Coughlan, T.W.Okita, H.Satoh, M.Ogawa, T.Kumamaru: "The rice mutant esp2 greatly accumulates the glutelin precursor and deletes the protein disulfide isomerase"Plant Physiology. 128. 1212-1222 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Qu L.Q., X.L.Wei, H.Satoh, T.Kumamaru, M.Ogawa, F.Takaiwa: "Inheritance of alleles for glutelin α-2 subunit genes in rice and identification of their corresponding cDNA"Theoretical and Applied Genetics. 105. 1099-1108 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Kumamaru, Y.Uemura, Y.Takemoto, M.Ogawa, H.Satoh: "High-resolution mapping of glup3 gene accumulating high amount of glutelin precursor"Rice Genetics Newsletter. 19(印刷中). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ushijima, T., H.Satoh, T.Kumamaru: "Mapping of esp1 gene for cystein-poor (CysP) prolamin decreased mutant in rice"Rice Genetics Newsletter. 19(印刷中). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Pa Pa Aung, T.Kumamaru, H.Satoh: "Genetic diversity of seed storage prolamins, in Myanmar local rice cultivars"Rice Genetics Newsletter. 19(印刷中). (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Issiki,M.,M.Nakajima,H.Satoh and K.Shimamoto: "dull : rice mutants with tissue-specific effects on the splicing of the waxy pre-mRNA."The Plant Journal. 23(4). 451-460 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Matsusaka,T.Kumamaru,M.Ogawa and H.Satoh: "Biosynthesis of rice S- poor and S- rich prolamins are regulated by independent genetic system."Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Takemoto,M.Ogawa,T.Kumamaru,T.W.Okita and H.Satoh: "Hypothetical model of genetic regulation of glutelin biosynthesis pathwey"Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.S.Jahan,T.Kumamaru,H.Satoh and H.Hamid: "Genetic diversity in seed storage proteins of Bangradesh rice cultivars"Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] P.P.Aung,T.Kumamaru and H.Satoh: "Genetic variation in seed storage protein and storage endosperm starch in Myanmar local rice cultivars."Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.U.Siddiqui,H.Satoh and T.Kumamaru: "Variation in the seed storage protein in Pakistan rice germplasm."Rice Genetics IV. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Satoh,Y.Uemura,T.C.Katayama,S.Minakuchi,T.Kumamaru and M.Ogawa: "Integration of Biodiversity and Genome Technology for Crop Improvement"National Institute of Agrobiologicl Resources, Tukuba 305 8602, Japan. 181 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2018-03-28  

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