His・Aspリン酸リレーによる大腸菌O157病原性遺伝子群の発現調節機構

• Project/Area Number: 12142206
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: 牧野 耕三 大阪大学, 微生物病研究所, 助教授 (20181620)
• Project Period (FY): 2000 – 2004
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥13,600,000 (Direct Cost: ¥13,600,000); Fiscal Year 2002: ¥6,800,000 (Direct Cost: ¥6,800,000); Fiscal Year 2001: ¥6,800,000 (Direct Cost: ¥6,800,000)
• Keywords: 二成分制御系 / His・Aspリン酸リレー / リン酸レギュロン / O-157 / ゲノム / PhoU / PhoB / PhoR / RhoR

Research Abstract

大腸菌におけるHis・Aspリン酸リレー情報伝達分子機構の解明を目的として、病原性大腸菌O-157特異的ゲノム解析を基にした二成分制御系による病原性遺伝子群解析と、リン酸レギュロンの二成分制御系による遺伝子発現ネットワーク解析により以下のことを明らかにした。
腸管出血性大腸菌O-157のゲノム長は5.6M塩基対でK12株と比べ、O-157特異的領域は約1.6Mである。この特異的領域に存在する二成分制御系をホモロジーに基づくコンピュータによる検索と解析をおこなった結果、K12株にはない二組のO-157特異的二成分制御系を発見して、これらの二成分制御系により発現が制御される遺伝子領域を詳細に明らかにした。さらに、二成分制御系のリン酸レギュロンにおけるモデュレーターPhoUのドミナントネガテブなphoU変異体を用いて、モデュレーターPhoUがおこなうリン酸レギュロンの負の調節機構に関して、PhoU側の機能部位を特定した。

Research Products

• [Publications] Shindoh, K.: "Crystallization and preliminary X-ray diffraction studies on the DNA-binding domain of the transcriptional activator protein PhoB from Escherichia coli"Acta Crystallogr.D.Biol.Crystallogr.. 58. 1862-1864 (2002)
• [Publications] Iida, T.: "Filamentous bacteriophages of vibrios are integrated into the dif-like site of the host chromosome"J.Bateriol.. 187. 4933-4935 (2002)
• [Publications] Makino, K.: "Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V. cholerae"Lancet. 361. 743-749 (2003)
• [Publications] Buck V.: "Peroxide sensors for the fission yeast stress-activated mitogen-activated protein kinase pathway"Mol.Biol.Cell. 12. 407-419 (2001)
• [Publications] Hayashi T.: "Complete genome sequence of enterohemorrhagic Escherichia coli O157 : H7 and genomic comparison with a laboratory strain K-12"DNA Res.(supplement, 47-52). 8. 11-22 (2001)
• [Publications] Okamura,H.: "Structural comparison of the PhoB and OmpR DNA-binding/transactivation domains and the arrangement of PhoB molecules on the phosphate box."J.Mol.Biol.. 295. 1225-1236 (2000)
• [Publications] Nasu,H.: "A filamentous phage associated with recent pandemic Vibrio parahaemolyticus O3'K6 strains."J.Clin.Microbiol.. 38. 2156-2161 (2000)
• [Publications] Raitt D.C.: "The Skn 7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress."Mol.Biol.Cell. 11. 2335-2347 (2000)
• [Publications] Tatsuno,I.: "Isolation and characterization of mini-Tn5Km2 insertion mutants of enterohemorrhagic Escherichia coli O157:H7 deficient in adherence to caco-2 cells."Infect.Immun.. 68. 5943-5952 (2000)
• [Publications] Kim,S-K.: "Dual transcriptional regulation of the Escherichia coli phosphate-starvation-inducible psiE gene of the phosphate regulon by PhoB and the cyclic AMP (cAMP)-cAMP receptor protein complex."J.Bacteriol.. 182. 5596-5599 (2000)
• [Publications] Yokoyama,K.: "Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the entrohemorrhagic Escherichia coli O 157:H7 strain derived from the Sakai outbreak."Gene. 258. 127-139 (2000)
• [Publications] Kawashima,T.: "Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium."Proc.Natl.Acad.Sci.USA.. 97. 14257-14262 (2000)