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金属が関与するセンサーとスイッチのケミカルバイオロジー

Research Project

Project/Area Number 12147207
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNippon Medical School

Principal Investigator

西野 武士  日本医科大学, 大学院・医学研究科, 教授 (40094312)

Co-Investigator(Kenkyū-buntansha) 石浜 明  (財)日本生物科学研究所, 主任研究員 (80019869)
斎藤 正男 (斉藤 正男 / 齋藤 正男)  東北大学, 多元物質科学研究所, 教授 (70302239)
石森 浩一郎  京都大学, 大学院・工学研究科, 助教授 (20192487)
岩崎 俊雄  日本医科大学, 医学部, 講師 (40277497)
岡本 研  日本医科大学, 医学部, 講師 (60267143)
新井 賢一  東京大学, 医科研, 教授(所長) (00012782)
三浦 謹一郎  (株)プロティオス研究所, 所長 (30000227)
京極 好正  産業技術総合研究所, センター長 (90012632)
Project Period (FY) 2000 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2004: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2003: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2002: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywords金属タンパク / 余属リセプター / 情報伝達金属タンパク質 / 情報スイッチ蛋白質 / COセンサー / O_2センサー / NOセンサー / スーパーオキシサイドセンサー / 金属タンパク質 / 金属リセプター / 情報スウィチ蛋白質 / スーパーオキサイドセンサー
Research Abstract

研究組織は総括班(研究代表者、その他の計画研究代表者2名、評価委員3名および事務担当その他の分担者)および計画研究代表者全体会議である。
1)平成16年6月19日(午後1時より)に日本医科大学において、計画研究代表者全員および分担研究者,評価委員2名の出席で全体会議を持つた。会議では、それぞれの計画研究代表者の今までの成果と今後の計画を発表し、班員の連係および最終年度のまとめおよび成果公開の具体的計画を立て確認した。
とくに成果公開を重点におくことそのための3回の公開および関連学会でのシンポジウム開催が確認された。
2)平成16年度公開シンポジウムを横浜で開催した。生化学会と連動させ実質二日間の国際シンポジウムとなった。公開シンポジウムは生化学会直前にもかかわらず海外からの参加者5名に加え、総数70名を超えた。
3)また生化学会は初日午前であったが会場はほぼ満席に近く、成果については科学新聞でも大きく取り上げられた。
4)生物物理学会において代表である西野および斉藤が中心となり金属蛋白質に関するシンポジウムを計画した。
5)平成17年1月7日(午後1時より7時まで)および8日(午前9時より12時まで)に日本医科大学において、計画研究代表者全員および分担研究者、評価委員1名の出席で全体会議を持った。総括班会議では、それぞれの計画研究の進展状況および各種シンポジウムの報告がなされ、研究の進展状況と問題点が検討された。全体会議では代表者の1年間の成果を発表し、詳しく討論された。さらに最終年度でもあり5年間の研究成果の集約にむけ研究のまとめをと今後の計画の討論を行なった。

Report

(5 results)
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (30 results)

All 2005 2004 Other

All Journal Article (6 results) Publications (24 results)

  • [Journal Article] Roles of distal Asp in heme oxygenase from Corynebacterium diphtheriae, HmuO : A water-driven oxygen activation mechanism2005

    • Author(s)
      T.Matsui, et al.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 2981-2989

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The fission yeast protein Ker1p is an ortholog of RNA polymerase I subunit A14 in Saccharomyces cerevisiae and is required for stable association of Rrn3p and RPA21 in RNA polymerase I2005

    • Author(s)
      Y.Imazawa, et al.
    • Journal Title

      J.Biol.Chem. 280・12

      Pages: 11467-11474

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli2005

    • Author(s)
      K.Yamamoto, et al.
    • Journal Title

      J.Biol.Chem. 280・2

      Pages: 1448-1456

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The crystal structure of xanthine oxidoreductase during catalysis : Implications for reaction mechanism and enzyme inhibition2004

    • Author(s)
      Ken Okamoto, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 101・21

      Pages: 7931-7936

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Y-700 [1-[3-Cyano-4-(2,2dimethylpropoxy)phenyl]-1H-pyrazole-4-carboxylic Acid] : A Potent Xanthine Oxidoreductase Inhibitor with Hepatic Excretion2004

    • Author(s)
      Atsushi Fukunari, et al.
    • Journal Title

      The Journal of Pharmacorogy and Experimental Therapeutics 311

      Pages: 519-528

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Crystal structure of the dioxygen-bound heme oxygenase from Corynebacterium diphtheriae : Implications for heme oxygenase function2004

    • Author(s)
      M.Unno, et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 21055-21061

    • Related Report
      2004 Annual Research Report
  • [Publications] Ishihama, A. et al.: "In vitro properties of RpoS (sS) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted."J.Bacteriol. 185(8). 2673-2679 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Makinoshima, H. et al.: "Growth phase-coupled alterations in cell structure and function of Escherichia coli."J.Bacteriol. 185(4). 1338-1345 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhang, X. et al.: "Stereoselectivity of each of the three steps of the heme oxygenase reaction : hemin to meso-hydroxyhemin, meso-hydroxyhemin to verdoheme, and verdoheme to biliverdin."Biochemistry. 42. 7418-7426 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Coyle, C.M.et al.: "FeNO structure in distal pocket mutants of myoglobin based on resonance Raman spectroscopy."Biochemistry. 42. 4896-4903 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuwabara, Y. et al.: "Unique relay system for switching from dehydrogenase to oxidase form of xanthine oxidoreductase."Proc.Natle.Acad, Scie.. 100. 8170-8175 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okamoto, K. et al.: "An Extremely Potent Inhibitor of Xanthine Oxidoreductase : Crystal Structure of the Enzyme-Inhibitor Complex and Mechanism of Inhibition"J.Biol.Chem.. 278. 1848-1855 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Akira, T.Atsuko, A.Wada, T.Nishino, A.Ishihama: "Systematic search for zinc-binding proteins in Esherichia coli"Eur. J. Biochm.. 269. 2403-2413 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Okamoto, B.T.Eger, T.Nishino, S.Kondo.B.F.Pai, T.Nishino: "An Extremely Potent Inhibitor of Xanthine Oxidoreductase : Crystal Structure of the Enzyme-Inhibitor Complex and Mechanism of Inhibition"J. Biol. Chem.. 278. 1848-1855 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Gonzalez, G., Dioum, E.M., Bertolucci, CM., Tomita, T.Ikeda-Saito, M., Cheesman, M.R., Watmough, N.J., Grilles-Gonzalez., M-A.: "Nature of the Displaceable Heme-Axial Residue in the EcDos Protein, a Heme-Based Sensor from Echerichia coli"Biochemistry. 41. 8414-8421 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tomita, T., Gonzalez, G., Chang, A.L., Ikeda-Saito, M., Gilles-Gonzalez, M.-A.: "A Comparative Resonance Raman Analysis of Heme-Binding PAS Domains : Heme Iron Coordination Structures of the BjFixL, AxPDEA1, EcDos, and MtDos Proteins"Biochemistry. 41. 4819-4826 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Ichida, T.Matsumura, R.Sakuma, T.Hosoya, T.Nishino: "Mutation of human molybdenum cofactor sulfurase gene is responsible for classical xanthinuria type II"Biochem. Biophys. Res. Commun.. 282. 1194-1200 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Cosper NJ, Scott RA, Hori H, Nishino T, Iwasaki T.: "X-ray absorption spectroscopic analysis of the high-spin ferriheme site in substrate-bound neuronal nitric-oxide synthase"J Biochem. 130(2). 191-198 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishino, T.: "Crystal structures of bovine milk xanthine dehydrogenase and mechanism of conversion from the dehydrogenase to the oxidase"Indian J. Chem. 41. 78-79 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishino, T., K.Okamoto, K., Pai, E.: "High-Resolution Structure of Bovine Milk Xanthine Oxidoreductase and Inhibitor Complexes"SPring-8 Research Frontiers. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Igarashi, I., Momohara, I., Nishino, T., Samejima, M.: "Kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus Phanerochaete chrysosporium"Biochem. J. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Pai, E., Nishino, T.: "Metal Ions in Biological Systems"Marcel Dekker, Inc., New York. 24 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Iwasaki,K.Okamoto,T.Nishino,J.Mizushima,H.Hori,and T.Nishino.: "Sequence Motif-specific Assignment of Two [2Fe-2S] Clusters in Rat Xanthine Oxidoreductase Studied by Site-directed Mutagenesis"J.Biochem.. 127. 771-778 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] C.Enroth,B.T.Eger,K.Okamoto,T.Nishino,T.Nishino,and E.F.Pai.: "Crystal Structures of Bovine Milk Xanthine Dehydrogenase and Xanthine Oxidase : Structure-Based Mechanism of Conversion"Proc.Natle.Acad.Sci.,USA. 97. 10723-10728 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] B.T.Eger,K.Okamoto,C.Enroth,M.Sato,T.Nishino,E.F.Pai,and T.Nishino.: "Purification,crystallization and preliminary X-ray diffraction studies of xanthine Dehydrogenase and xanthine Oxidase isolated from bovine milk."Acta Cryst.. D56. 1656-1658 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] JHwang,J.-S.,Yamda,K.,Honda,A.,Nakade,K.and Ishihama,A.: "Expression of functional influenza viral RNA polymerase in the methylotrophic yeast Pichia pastoris."J.Virol.. 74. 4074-4084 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishiguro,A.,Nogi,Y.,Hisatake,K.,Muramatsu,M.and Ishihama,A: "Ppb6 subunit of the fission yeast RNA polymerase II is a contact target of the transcription elongation factor TFIIS."Mol.Cell.Biol.. 20. 1263-1270 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ishihama,A: "Functional modulation of Escherichia coli RNA polymerase."Ann.Rev.Microbiol.. 54. 499-518 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ghu G.C.,Katakura,K.,Tomita,T.,Zhang,X.,Sun,D.,Sato,M.,Sasahara,M.,Kayama,T.,Ikeda-Saito,M.,and Yoshida,T.: "Histidine 20, the Crucial Proximal Axial Heme Ligand of Bacterial Heme Oxygenase Hmu O from Corynebacterium diphtheriae."J.Biol.Chem.. 275. 17494-17500 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomita T.,Haruta,N.,Aki,M.,Kitagawa,T.,and Ikeda-Saito,M.: "UV Resonance Raman Ditection of a Ligand Vibration on Ferric Nitrosyl Heme Proteins""J.Am.Chem.Soc.. 123(in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2018-03-28  

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