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Analysis of developmental genetic system using small teleost fish as vertebrate model systems

Research Project

Project/Area Number 12202004
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Tokyo (2001-2004)
National Institute of Genetics (2000)

Principal Investigator

TAKEDA Hiroyuki  University of Tokyo, Graduate School of Science, 大学院理学系研究科, 教授 (80179647)

Co-Investigator(Kenkyū-buntansha) NARUSE Kiyoshi  University of Tokyo, Graduate School of Science, 大学院理学系研究科, 講師 (50208089)
HORI Hiroshi  Nagoya University, Graduate School of Science, 大学院理学研究科, 教授 (60116663)
KUDO Akira  Tokyo Institute of Technology, Department of Biological information, 生命理工研究科, 教授 (70178002)
Project Period (FY) 2000 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥208,800,000 (Direct Cost: ¥208,800,000)
Fiscal Year 2004: ¥54,400,000 (Direct Cost: ¥54,400,000)
Fiscal Year 2003: ¥54,400,000 (Direct Cost: ¥54,400,000)
Fiscal Year 2002: ¥48,000,000 (Direct Cost: ¥48,000,000)
Fiscal Year 2001: ¥52,000,000 (Direct Cost: ¥52,000,000)
Keywordsmedaka / genome / EST / Hox / mutant / development / evolution / SNP / SNPS / Hox cluster / Hox / 器官形成 / BAC
Research Abstract

In the present study, we focused on the medaka that became an attractive model organism for the study of vertebrate early development and genome evolution. The following three groups were jointed, aiming at the establishment of comprehensive resources for medaka developmental genetics as well as structural and functional analyses of developmentally important genes in medaka.
1. Takeda and Naruse
To strengthen the study of medaka developmental genetics, Takeda's laboratory has conducted a large-scale isolation of ESTs from medaka embryos and developed tools that facilitate mutant analysis such as rapid mapping system, M-markers and microarray with 8,000 uni-genes. These resources are accelerating medaka mutant analyses and made an important contribution to the medaka genome sequencing project.
2. Hori
Hori's laboratory analyzed the organization of the entire hox gene loci. Together with the information in the Fugu genome database and in the Danio genome database, the physical maps of three fish genomes were constructed and compared one another. Not only numbers of hox genes but also the distances between the neighboring hox genes are highly similar between medaka and fugu. As for six clusters, Aa, Ab, Ba, Bb, Ca and Da that are commonly present in the three fishes, only few or no differences were found in each cluster. Thus, the hox gene sets should have been well conserved once they had been established in respective species.
3. Kudo
Kudo's laboratory has screened for mutants that are defective in organogenesis and fin regeneration in the medaka, using ethylnitrosourea (ENU) as a mutagen. A total of 386 mutagenized genomes were scored to identify 151 mutations affecting organogenesis, and 16 mutations affecting the fin regeneration were identified. In parallel, they isolated 20,000 ESTs from re-generating fins, representing about 3,000 uni-genes.

Report

(6 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (74 results)

All 2005 2004 2002 2001 Other

All Journal Article (29 results) Publications (45 results)

  • [Journal Article] Identification of a gene required for de novo DNA methylation of the zebrafish no tail gene2005

    • Author(s)
      Shimoda, N.
    • Journal Title

      Developmental Dynamics 233

      Pages: 1509-1516

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic linkage map of medaka with polymerase chain reaction length polymorphisms2005

    • Author(s)
      Kimura, T.
    • Journal Title

      Gene 19

      Pages: 24-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Retinoic acid-metabolizing enzy;me Cyp26al is essential for determining territories of hindbrain and spinal cord in zebrafish2005

    • Author(s)
      Emoto, Y.
    • Journal Title

      Dev. Biol. 278

      Pages: 415-427

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic linkage map of medaka with polymerase chain reaction length polymorphisms2005

    • Author(s)
      Kimura, T.
    • Journal Title

      Gene 19(363)

      Pages: 24-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Retinoic acid-metabolizing enzy ; me Cyp26al is essential for determining territories of hindbrain and spinal cord in zebrafish2005

    • Author(s)
      Emoto, Y.
    • Journal Title

      Dev. Biol. 278

      Pages: 415-427

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Retinoic acid-metabolizing enzyme Cyp26a1 is essential for determining territories of hindbrain and spinal cord in zebrafish.2005

    • Author(s)
      Emoto, Y.
    • Journal Title

      Developmental Biology 278

      Pages: 415-427

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Large-scale isolation of ESTs from medaka embryos and its application to medaka developmental genetics2004

    • Author(s)
      Kimura, T.
    • Journal Title

      Mechanisms of Development 121

      Pages: 915-932

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Early fin primordia of zebrafish larvae regenerate by a similar growth control mechanism with adult regeneration2004

    • Author(s)
      Kawakami A.
    • Journal Title

      Developmental Dynamics 231(4)

      Pages: 693-699

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A mutation in the gene for delta-aminolevulinic acid dehydratase (ALAD) causes hypochromic anemia in the medaka, Oryzias latipes2004

    • Author(s)
      Sakamoto, D.
    • Journal Title

      Mechanisms of Development 121

      Pages: 747-752

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of mutations affecting embryonic hematopoiesis in the medaka, Oryzias latipes2004

    • Author(s)
      Tanaka, K.
    • Journal Title

      Mechanisms of Development 121

      Pages: 739-746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Large-scale analysis of the genes involved in fin regeneration and blastema formation in the medaka, Oryzias latipes2004

    • Author(s)
      Katogi, R.
    • Journal Title

      Mechanisms of Development 121

      Pages: 861-872

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The novel medaka transglutaminase gene is expressed in developing yolk veins2004

    • Author(s)
      Koh, D.
    • Journal Title

      Gene Exp. Patterns. 4

      Pages: 263-266

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Zebrafish periostin is required for the adhesion of muscle fiber bundles to the myoseptum and for the differentiation of muscle fibers2004

    • Author(s)
      Kudo, H.
    • Journal Title

      Developmental Biolology 267

      Pages: 473-487

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Medaka genomics : a bridge between mutant phenotypes and gene function2004

    • Author(s)
      Naruse, K.
    • Journal Title

      Mechanisms of Development 121

      Pages: 619-628

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Large-scale isolation of ESTs from medaka embryos and its application to medaka developmental genetics.2004

    • Author(s)
      Kimura, T
    • Journal Title

      Mechanisms of Development 121

      Pages: 915-932

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Medaka genomics : a bridge between mutant phenotypes and gene function.2004

    • Author(s)
      Naruse, K.
    • Journal Title

      Mechanisms of Development 121

      Pages: 629-638

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Early fin primordia of zebrafish larvae regenerate by a similar growth control mechanism with adult regeneration.2004

    • Author(s)
      Kawakami A
    • Journal Title

      Developmental Dynamics 231(4)

      Pages: 693-699

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of mutations affecting embryonic hematopoiesis in the medaka, Oryzias latipes.2004

    • Author(s)
      Tanaka, K
    • Journal Title

      Mechanisms of Development 121

      Pages: 739-746

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Large-scale analysis of the genes involved in fin regeneration and blastema formation in the medaka, Oryzias latipes.2004

    • Author(s)
      Katogi, R
    • Journal Title

      Mechanisms of Development 121

      Pages: 861-872

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Tbx24, a novel T-box gene, is mutated in the zebrafish somite segmentation mutant fused somites2002

    • Author(s)
      Nikaido, M.
    • Journal Title

      Nature Genetics 31

      Pages: 5-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DMY is a Y-specific DM-domain gene required for male development in the medaka fish2002

    • Author(s)
      Matsuda M.
    • Journal Title

      Nature 417(6888)

      Pages: 559-563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DMY is a Y-specific DM-domain gene required for male development in the medaka fish2002

    • Author(s)
      Matsuda, M.
    • Journal Title

      Nature 417(6888)

      Pages: 559-563

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The Tol2 transposable element of the medaka fish : an active DNA-based element naturally occurring in a vertebrate genome. (Review)2001

    • Author(s)
      Koga A.
    • Journal Title

      Genes Genet Syst. 76(1)

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of the endoderm and gut in medaka, Oryzias latipes

    • Author(s)
      Kobayashi, D.
    • Journal Title

      Development, Growth and Differentiation (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transgenic analysis of medaka mesp-b enhancer in somitogenesis

    • Author(s)
      Terasaki, H.
    • Journal Title

      Development, Growth and Differentiation (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes

    • Author(s)
      Kurosawa, G.
    • Journal Title

      Gene (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of the endoderm and gut in medaka, Oryzias latipes

    • Author(s)
      Kobayashi, D.
    • Journal Title

      Development, Growth and Differentiation (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transgenic analysis of medaka mesp-b enhancer in somitogenesis

    • Author(s)
      Terasaki, H.
    • Journal Title

      Development, Growth and Differentiation (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Organization and structure of hox gene loci in medaka genome and comparison with those of pufferfish and zebrafish genomes

    • Author(s)
      Kurosawa, G.
    • Journal Title

      Gene (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Takeuchi, M.: "The prickle-related gene in vertebrates is essential for gastrulation cell movements"Current Biology. 13. 674-679 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kanamori, A.: "Genomic organization of ZP domain containing egg envelope genes in medaka (Oryzias latipes)"Gene. 305. 35-45 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tsustsumi, M.: "Long and short mRNAs transcribed from the medaka fish transposon To12 respectively exert positive and negative effects on excision"Genetic Research. 82. 33-40 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kudo, H.: "Zebrafish periostin is required for the adhesion of muscle fiber bundles to the myoseptum and for the differentiation of muscle fibers"Developmental Biology. 267. 473-487 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sekimizu, K.: "The zebrafish iguana locus encodes Dzipl, a novel zinc finger protein required for proper regulation of hedgehog signaling"Development. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kimura, T.: "Large-scale isolation of ESTs from medaka embryos and its application to medaka developmental genetics"Mechanisms of Development. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Naruse, K.: "Medaka genomics : a bridge between mutant phenotypes and gene function"Mechanisms of Development. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Okubo: "Structural characterization of GnRH loci in the medaka genome"Gene. 293(1-2). 181-189 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Okubo: "Conserved physical linkage of GnRH-R and RBM8 in the medaka and human genomes"Bioche. Biophy. Res. Com. 293:(1). 327-331 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Kondo: "Molecular cloning and characterization of DMRT genes from the medaka Oryzias latipes and the platyfish Xiphophorus maculates"Gene. 295(2). 213-222 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Kanamori: "Genomic organization of ZP domain containing egg envelope genes in medaka (Oryzias latipes)"Gene. 305. 35-45 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Aizawa: "The medaka midblastula transition as revealed by the expression of the paternal genome"Gene Expression Patterns. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koga A: "Tansposable element in medaka fish. (Review)"Zool Science. 19(1). 1-6 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsuda M: "DMY is a Y-specific DM-domain gene required for male development in the medaka fish"Nature. 417. 559-563 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koga A: "Gene transfer and cloning of flanking chromosornal regions using medaka fish Tol2 transposable element"Marine Biotechnology. 4. 6-11 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koga A: "Gamera, a family of LINE-like repetitive sequences widely distributed in medaka and related fishes"Heredity. 89(6). 446-452 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamagami S: "Expression and genomic organization of a medaka fish novel membrane form of guanylyl cyclase/orphan receptor"Zool Science. 20(1)(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nikaido, M: "Tbx24, a novel T-box gene, is mutated in the zebrafish somite segmentation mutant fused somites"Nature Genetics. 31. 195-199 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Picker, A: "A novel positive transcriptional feed back loop in midbrain-hindbrain boundary development is revealed through analysis of the zebrafish pax2.1 promoter in transgenic lines"Development. 129. 3227-3239 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yokoi, H: "sox9 in a teleost fish, medaka (Orizias latipes) : evidence for diversified function of sox9 in gonad differentiation"Molecular Reproduction and Development. 63. 5-16 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tonou-Fujimori, N: "Expression of the FGF receptor 2 gene (fgfr2) during embryogenesis in the zebrafish Danio rerio"Gene Expression Patterns. 2. 183-188 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sakaguchi, T: "Formation and patterning roles of the yolk syncytial layer. (Review)"Pattern Formation in Zebrafish. 1-14 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Holley, S.A: "Catching a wave : the oscillator and wavefront that create the zebrafish somite. (Review)"Seminars in Cell & Developmental Biology. 13. 481-488 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takuya Sakaguchi: "Expression of Zebrafish btg-b, an anti-proliferative cofactor, during early enbryogenesis"Mechanisms of Development. 104. 113-115 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takuya Sakaguchi: "A novel sox gene, 226D7, acts downstream of Nodal signaling to specify endoderm precursors in zebrafish"Mechanism of Development. 107. 25-38 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Minori Shinya: "Fgf signalling through MAPK cascade is required for development of the subpallial telencephalon in zebrafish embryos"Development. 128. 4153-4154 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Atsushi Sawada: "Fgf/YMAPK signalling is a crucial positional cue in somite boundary formation"Development. 128. 4873-4880 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yumiko Saga: "The making of the somite : molecular events in vertebrate segmentation"Nature Reviews Genetics. 2. 835-845 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sumito Koshida: "Inhibition of BMP activity by the FGF signal promotes posterior neural development in zebrafish"Developmental Biology. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 成瀬 清: "ゲノム時代を泳ぎ抜く -小型魚類"細胞工学. 22. 56-62 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masaru Matsuda: "Construction of a BAC library derived from the inbred Hd-rR strain of the teleost fish, Oryzias latipes"Genes Genetic System. 76(1). 61-63 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akihiko Koga: "The Tol2 transposable element of the medaka fish : an active DNA-based element naturally occurring in a vertebrate genome. (Review)"Genes Genetic System. 76(1). 1-8 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akihiko Koga: "Transpozable element in medaka fish. (Review)"Zoological Science. 19(1). 1-6 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akihiko Koga: "Gene transfer and cloning of flanking chromosomal regions using medaka fish Tol2 transposable element"Marine Biotechnology. 4(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 武田 洋幸: "動物のからだづくり -形態発生の分子メカニズム-"朝倉書店. 138 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Atsushi Sawada: "Zebrafish Mesp family genes, mesp-a and mesp-b are segmentally expressed in the presomitic mesoderm, and Mesp-b confers the anterior identity to the developing somites."Development. 128. 1691-1702 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hisato Kondo: "Zebrafish mutations in Gli mediated hedgehog signaling lead to lens transdifferentiation from the adenohypophysis anlage."Mechanisms of Development. 96. 165-174 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takuya Sakaguchi: "Expression of zebrafish BTG-b, anti-proliferative cofactor, during early embryogenesis"Mechanisms of Development. in press. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akihiko Koga: "The medaka fish transpozable element Tol2 : Low level of interspecific sequence divergence points to recent invation of the fish genome"Genetics. 155. 273-281 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akihiko Koga: "Detection of de novo insertion of the medaka fish transposable element Tol2"Genetics. 155. 1243-1247 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masaru Matsuda: "Construction of a BAC library derived from the inbred Hd-rR strain of the teleost fish,Orizias latipes"Genes & Genetic Sysmtems. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akihiko Koga: "The Tol2 transposable element of the medaka fish : an active DNA-based element naturally occurring in a vertebrate geneoms"Genes & Genetic Sysmtems. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 澤田篤志: "体節の形成と分化"蛋白質核酸酵素. 45. 2766-2773 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 新屋みのり: "神経誘導と神経組織の体軸に沿ったパターニング"蛋白質核酸酵素. 45. 2738-2744 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 黒田仁: "魚類のゲノムとHox遺伝子群の進化"蛋白質核酸酵素. 45. 2893-2899 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2021-12-17  

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