| Project/Area Number |
12204003
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
SUGANO Sumio THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF FRONTIER SCIENCES DEPARTMENT OF MEDICAL GENOME SCIENCES LABORATORY OF FUNCTIONAL GENOMICS, PROFESSOR, 大学院新領域創成科学研究科, 教授 (60162848)
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| Co-Investigator(Kenkyū-buntansha) |
OHMI Shinobu THE UNIVERSITY OF TOKYO, THE INSTITUTE OF MEDICAL SCIENCE, ASSOCIATE PROFESSOR, 医科学研究所, 助教授 (20160046)
KATO Hiroyuki NATIONAL INSTITUTE OF INFECTIONS, DISEASES DEPARTMENT OF VIROLOGY III, SENIOR INVESTIGATOR, ウィルス第三部, 主任研究官 (60185866)
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| Project Period (FY) |
2000 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥96,400,000 (Direct Cost: ¥96,400,000)
Fiscal Year 2004: ¥24,000,000 (Direct Cost: ¥24,000,000)
Fiscal Year 2003: ¥24,000,000 (Direct Cost: ¥24,000,000)
Fiscal Year 2002: ¥24,000,000 (Direct Cost: ¥24,000,000)
Fiscal Year 2001: ¥24,400,000 (Direct Cost: ¥24,400,000)
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| Keywords | Full-Length of cDNA / Transcription / Promoter / SNP / Expression Profile / オリゴキャップ法 / EST / 5'端 / 転写開始点 / 転写因子 / TATA-BOX / ドラフト配列 |
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| Research Abstract |
We made cDNA libraries using oligocapping method of various normal tissues, tumors and cell lines. From those, we isolated about 1.65 million cDNA clones and determined their 5' EST sequence. Using about 1,5 million 5'EST data, we did clustering and mapping to human genome sequence. As the result, we could determine the transcriptional start sites for 19,000 known genes. We picked up up-stream 1000bp region for the putative promoters of those genes.
We made database DBTSS using above data and made public through web sites.
We also cloned promoter regions of about 500 genes and determined their activity in cultured cells. Similarly, we cloned random genomic fragments which do not include any promoter regions and assayed their activity. About 10% of the clones showed weak promoter activity. We currently think this observation may correspond to the abundance of the non-coding RNA found among the transcripts.
We also performed proteome analysis of the small sized protein. The result showed a possibility that small ORF present in 5'UTR may actually directs the synthesis of small proteins. We determined the subcellular localization of 3000 proteins whose function is not yet determined.
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