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Genome analysis of the cellular slime molds to understand the genetic system controlling cell differentiation

Research Project

Project/Area Number 12206001
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Tsukuba

Principal Investigator

URUSHIHARA Hideko (2003-2004)  University of Tsukuba, Graduate school of Life and Environmental sciences, Professor, 大学院生命環境科学研究科, 教授 (00150087)

田仲 可昌 (2000-2002)  筑波大学, 生物科学系, 教授 (80091908)

Co-Investigator(Kenkyū-buntansha) TAKAKA Yoshimasa  University of Tsukuba, Graduate school of Life and Environmental sciences, Professor, 大学院生命環境科学研究科, 教授 (80091908)
MORIO Takahiro  University of Tsukuba, Graduate school of Life and Environmental sciences, Assistant Professor, 大学院生命環境科学研究科, 助手 (10292509)
MAEDA Mineko  Osaka University, Graduate School of Sciences, Associate Professor, 大学院理学研究科, 助教授 (70029700)
OCHIAI Hiroshi  Hokkaido University, Graduate School of Sciences, Professor, 大学院理学研究科, 教授 (10002122)
漆原 秀子  筑波大学, 生物科学系, 助教授 (00150087)
Project Period (FY) 2000 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥136,800,000 (Direct Cost: ¥136,800,000)
Fiscal Year 2004: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 2003: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 2002: ¥33,800,000 (Direct Cost: ¥33,800,000)
Fiscal Year 2001: ¥43,000,000 (Direct Cost: ¥43,000,000)
KeywordsDictyostelium discoideum / EST analysis / Cell differentiation / Gene expression / Microarray analysis / transcription factors / Upstream sequences / Cis-elements / 遺伝子発現 / 遺伝子ネットワーク / 脱分化 / 転写制御 / 配列アセンブル / DNAマイクロアレイ / cDNA解析 / ゲノム / 細胞分化 / マイクロアレイ / in situハイブリダイゼーション / 転写制御因子 / 完全長cDNA / ポリAトラップ法
Research Abstract

The cellular slime mold, Dictyostelium discoideum, was used to understand the genetic control of development and differentiation. This organism normally proliferates as unicellular amoebae but gathers and constructs multicellular structures upon starvation. First, large scale analysis of cDNAs was performed to establish the genetic resources. Fourteen cDNA libraries were constructed from the cells at 5 developmental stages, and the nucleotide sequences of 95,884 clones, including 72,703 full-length ones, were determined. The sequences were assembled into 6,790 independent genes, which corresponded to 54% of the predicted 12,500 genes. These resources were then used for reverse-genetic and bioinformatic analyses. The following results were obtained.
1. Gene expression patterns during normal development were obtained showing occurrence of the overall drastic changes in expressed genes after cell aggregation. Whole mount in situ hybridization revealed the fine pattern formation in the prestalk region.
2. Two hundred and sixty genes were extracted to encode probable transcription factors, and precise expression patterns were obtained by real-time PCR for 40 of them. Thirteen genes were disrupted.
3. The Genetic network among 9 probable transcription factors was obtained using the S-system calculation based on the gene expression patterns during development in the wild type and disruption mutants.
4. Upstream promoter sequences were obtained by aligning the full-length cDNA and genome sequences, and candidate cis regulatory elements were extracted referring to the expression specificities.

Report

(6 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (93 results)

All 2006 2005 2004 2003 2002 2001 2000 Other

All Journal Article (71 results) Book (2 results) Publications (20 results)

  • [Journal Article] Dictyostelium transcriptional host cell response upon infection with Legionella.2006

    • Author(s)
      Farbrother, P.
    • Journal Title

      Cell. Microbiol. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion2006

    • Author(s)
      Muramoto, T.
    • Journal Title

      Dev. Growth Differ. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evidence that the Dictyostelium STAT protein Dd-STATa plays a role in the differentiation of inner basal disc cells and identification of a promoter element essential for expression in these cells.2005

    • Author(s)
      Shimada, N.
    • Journal Title

      Differentiation 73

      Pages: 50-60

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] On the development of an analysis system for upstream sequences in Dictyostelium discoideum genome.2005

    • Author(s)
      Kobayashi, N.
    • Journal Title

      コンピュータソフトウェア 22

      Pages: 167-172

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signaling pathway in Dictyostelium discoideum.2005

    • Author(s)
      Muramoto, T.
    • Journal Title

      Mech. Dev. 122

      Pages: 733-743

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The genome of the social amoeba Dictyostelium discoideum.2005

    • Author(s)
      Eichinger, L.
    • Journal Title

      Nature 435

      Pages: 43-57

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa.2005

    • Author(s)
      Rivero, F.M.T.
    • Journal Title

      BMC Genom. 6

      Pages: 28-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evidence that the Dictyostelium STAT protein Dd-STATa plays a role in the differentiation of inner basal disc cells and identification of a promoter element essential for expression in these cells.2005

    • Author(s)
      Shimada, N., Kawata, T., Maruo, T., Maeda, M., Urushihara, H.
    • Journal Title

      Differentiation 73

      Pages: 50-60

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] On the development of an analysis system for upstream sequences in Dictyostelium discoideum genome.2005

    • Author(s)
      Kobayashi, N., Marin, M., Tanaka, Y., Urushihara, H.
    • Journal Title

      Computor Softwear 22(3)

      Pages: 167-172

    • NAID

      130005006570

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signalling pathway in Dictyostelium discoideum.2005

    • Author(s)
      Muramoto, T., Takeda, S., Furuya, Y., Urushihara, H.
    • Journal Title

      Mech.Dev. 122

      Pages: 733-743

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The genome of the social amoeba Dictyostelium discoideum.2005

    • Author(s)
      Eichinger, L., Pachebat, J.A., Glockner, G, Rajandream, M.-A., Sucgang, R., Berriman, M., Song, J., Olsen, R., Szafranski, K., Xu, Q.et al.
    • Journal Title

      Nature 435

      Pages: 43-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa.2005

    • Author(s)
      Rivero, F.M.T., Meyer, A.K., Urushihara, H., Uyeda, T.Q., Kitayama, C
    • Journal Title

      BMC Genom. 6(1)

      Pages: 28-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evidence that the Dictyostelium STAT protein Dd-STATa plays a role in the differentiation of inner basal disc cells and identification of a promoter element essential for expression in these cells2005

    • Author(s)
      Shimada, N. et al.
    • Journal Title

      Differentiation (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] An orderly retreat : dedifferentiation is a regulated process.2004

    • Author(s)
      Katoh, M.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 101

      Pages: 7005-7010

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum.2004

    • Author(s)
      Urushihara, H.
    • Journal Title

      Nuc. Acids Res. 32

      Pages: 1647-1653

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Extracellular matrix family proteins that are potential targets of Dd-STATa in Dictyostelium discoideum.2004

    • Author(s)
      Shimada, N.
    • Journal Title

      J. Plant Res. 117

      Pages: 345-353

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridization.2004

    • Author(s)
      Shimada, N.
    • Journal Title

      Int. J. Dev. Biol. 48

      Pages: 679-682

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium.2004

    • Author(s)
      Matsuoka, S.
    • Journal Title

      Dev. Growth Differ. 45

      Pages: 195-199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Control of cell-type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization.2004

    • Author(s)
      Maruo, T.
    • Journal Title

      Eukaryot. Cell 3

      Pages: 1241-1248

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An orderly retreat : dedifferentiation is a regulated process.2004

    • Author(s)
      Katoh, M., Shaw, C., Van Driessche, N., Xu, Q., Morio, T., Kuwayama, H., Obara, S., Urushihara, H., Tanaka, Y., Shaulsky, G.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 7005-7010

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum.2004

    • Author(s)
      Urushihara, H., Morio, T., Saito, T., Kohara, Y., Kouriki, E., Ochiai, H., Maeda, M., Williams, J.G, Takeuchi, I., Tanaka, Y.
    • Journal Title

      Nuc.Acids Res. 32

      Pages: 1647-1653

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Extracellular matrix family proteins that are potential targets of Dd-STATa in Dictyostelium discoideum.2004

    • Author(s)
      Shimada, N., Nishino, K., Maeda, M., Urushihara, H., Kawata, T.
    • Journal Title

      J.Plant Res. 117

      Pages: 345-353

    • NAID

      10013737062

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridization.2004

    • Author(s)
      Shimada, N., Maeda, M., Urushihara, H., Kawata, T.
    • Journal Title

      Int.J.Dev.Biol. 48

      Pages: 679-682

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Defect in peroxisomal multifunctional enzyme MFE1 affects cAMP relay in Dictyostelium2004

    • Author(s)
      Matsuoka, S., Kuwayama, H., Ikeno, D., Oyama, M., Maeda, M.
    • Journal Title

      Dev.Growth Differ. 45

      Pages: 195-199

    • NAID

      10012907986

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Control of cell-type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization.2004

    • Author(s)
      Maruo, T., Sakamoto, S., Iranfar, N., Fuller, D., Morio, T., Urushihara, H., Tanaka, Y., Maeda, M., F.Loomis, W.F.
    • Journal Title

      Eukaryot.Cell 3

      Pages: 1241-1248

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An orderly retreat : Dedifferentiation is a regulated process.2004

    • Author(s)
      Katoh, M. et al.
    • Journal Title

      Proc.Nat.Acad.Sci.USA 101(18)

      Pages: 7005-7010

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridisation.2004

    • Author(s)
      Shimada, N. et al.
    • Journal Title

      Int.J.Dev.Biol. 48

      Pages: 679-682

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Extracellular matrix family proteins that are poteintial targets of Dd-STATa in Dictyostelium discoideum.2004

    • Author(s)
      Shimada, N. et al.
    • Journal Title

      J. Plant Res. 117

      Pages: 345-353

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum2004

    • Author(s)
      Urushihara, H. et al.
    • Journal Title

      Nuc. Acids Res. 32(5)

      Pages: 1647-1655

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signaling pathway in Dictyostelium discoideum2004

    • Author(s)
      Muramoto, T. et al.
    • Journal Title

      Mech.Dev. (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Changing patterns of gene expression in prestalk cell subtypes of Dictyostelium recognized by in situ hybridization with genes from microarray analyses.2003

    • Author(s)
      Maeda, M.
    • Journal Title

      Eukaryot. Cell 2

      Pages: 627-637

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp.2003

    • Author(s)
      Matsuoka, S.
    • Journal Title

      Eukaryot. Cell 2

      Pages: 638-645

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Construction of a gamete-enriched gene pool and RNAi- mediated functional analysis in Dictyostelium discoideum.2003

    • Author(s)
      Muramoto, T.
    • Journal Title

      Mech. Dev. 120

      Pages: 965-975

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during development.2003

    • Author(s)
      Sakamoto, S.
    • Journal Title

      Dev. Growth Differ. 45

      Pages: 507-514

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel gene trap method using terminator-REMI and 3'rapid amplification of cDNA ends (RACE) in Dictyostelium.2003

    • Author(s)
      Takeda, K.
    • Journal Title

      Gene 312

      Pages: 321-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A STAT regulated, stress-induced signalling pathway in Dictyostelium.2003

    • Author(s)
      Araki, T.
    • Journal Title

      J. Cell Sci. 116

      Pages: 2907-2915

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changing patterns of gene expression in prestalk cell subtypes of Dictyostelium recognized by in situ hybridization with genes from microarray analyses.2003

    • Author(s)
      Maeda, M., Sakamoto, S., Iranfar, N., Fuller, D., Maruo, T., Ogihara, S., Morio, T., Urushihara, H., Tanaka, Y., Loomis, W.L.
    • Journal Title

      Eukaryot.Cell 2

      Pages: 627-637

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] MFE1, a member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family, affects fatty acid metabolism necessary for morphogenesis in Dictyostelium spp.2003

    • Author(s)
      Matsuoka, S., Saito, T., Kuwayama, H., Morita, N., Ochiai, H., Maeda, M.
    • Journal Title

      Eukaryot.Cell 2

      Pages: 638-645

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum.2003

    • Author(s)
      Muramoto, T., Suzuki, K., Shimizu, H., Kohara, Y., Kohriki, E., Obara, S., Tanaka, Y., Urushihara, H.
    • Journal Title

      Mech.Dev. 120

      Pages: 965-975

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during development.2003

    • Author(s)
      Sakamoto, S., Nishino, K., Tomisako, M., Kuwayama, H., Tanaka, Y., Suetake, I., Tajima, S., Ogihara, S., Coukell, B., Maeda, M.
    • Journal Title

      Dev.Growth Differ. 45

      Pages: 507-514

    • NAID

      10012005774

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium.2003

    • Author(s)
      Takeda, K., Saito, T., Tanaka, T., Morio, T., Maeda, M., Tanaka, Y., Ochiai, H.
    • Journal Title

      Gene 312

      Pages: 321-333

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A STAT regulated, stress-induced signalling pathway in Dictyostelium.2003

    • Author(s)
      Araki, T., Tsujioka, M., Abe, A., Fukuzawa, M., Meima, M., Schaap, P., Morio, T., Urushihara, H., Katoh, M., Maeda, M., Tanaka, Y., Takeuchi, I., Williams, J.G
    • Journal Title

      J.Cell Sci. 116

      Pages: 2907-2915

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.2002

    • Author(s)
      Kuwayama, H.
    • Journal Title

      Nuc. Acids Res. 30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A transcriptional profile of multicellular development in Dictyostelium discoideum.2002

    • Author(s)
      Van Driessche, N.
    • Journal Title

      Development 129

      Pages: 1543-1552

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel PCR-mediated method for one tube generation of a gene disruption construct.2002

    • Author(s)
      Kuwayama, H.
    • Journal Title

      Biotech. Lett. 24

      Pages: 1307-1312

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Syntaxin 7, syntaxin 8, Vtil and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum.2002

    • Author(s)
      Bogdanovic, A.
    • Journal Title

      Biochem. J. 368

      Pages: 29-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional Genomics of the social amoebae, Dictyostelium discoideum.2002

    • Author(s)
      Urushihara, H.
    • Journal Title

      Mol. Cells 13

      Pages: 1-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.2002

    • Author(s)
      Kuwayama, H., Obara, O., Morio, T., Katoh, M., Urushihara, H., Tanaka, Y.
    • Journal Title

      Nuc.Acids Res. 30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A transcriptional profile of multicellular development in Dictyostelium discoideum.2002

    • Author(s)
      Van Driessche, N., Shaw, C., Katoh, M., Morio, T., Sucgang, R., Ibarra, M., Kuwayama, H., Saito, T., Urushihara, H., Maeda, M., Takeuchi, I., Ochiai, H., Eaton, W., Tollett, J., Halter, J., Kuspa, K., Tanaka, Y., Shaulsky, G.
    • Journal Title

      Development 129

      Pages: 1543-1552

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel PCR-mediated method for one tube generation of a gene disruption construct.2002

    • Author(s)
      Kuwayama, H., Obara, S., Morio, T., Katoh, M., Kuhara, K., Tanaka, Y.
    • Journal Title

      Biotech.Lett. 24

      Pages: 1307-1312

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum.2002

    • Author(s)
      Bogdanovic, A., Bennett, N., Kieffer, S., Louwagie, M., Morio, T., Garin, J., Satre, M., Bruckert, F.
    • Journal Title

      Biochem.J. 368

      Pages: 29-39

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional Genomics of the social amoebae, Dictyostelium discoideum.2002

    • Author(s)
      Urushihara, H.
    • Journal Title

      Mol.Cells 13

      Pages: 1-4

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of two penta-EF-hand Ca2+-binding proteins in Dictyostelium discoideum.2001

    • Author(s)
      Ohkouchi, S.
    • Journal Title

      J. Biochem. 130

      Pages: 207-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ORI-GENE : gene classification based on the evolutionary tree.2001

    • Author(s)
      Mizuno, H.
    • Journal Title

      Bioinformatics 17

      Pages: 167-173

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium development.2001

    • Author(s)
      Tsujioka, M.
    • Journal Title

      Dev. Growth & Differ. 43

      Pages: 275-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FebA : a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum.2001

    • Author(s)
      Morio, T.
    • Journal Title

      Biochim. Biophys. Acta 1519

      Pages: 65-69

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of two penta-EF-hand Ca2+-binding proteins in Dictyostelium discoideum.2001

    • Author(s)
      Ohkouchi, S., Nishio, K., Maeda, M., Hitomi, K., Adachi, H., Maki, M.
    • Journal Title

      J.Biochem. 130

      Pages: 207-215

    • NAID

      130003534226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ORI-GENE : gene classification based on the evolutionary tree.2001

    • Author(s)
      Mizuno, H., Tanaka, Y., Nakai, K., Sarai, A.
    • Journal Title

      Bioinformatics 17(2)

      Pages: 167-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium development.2001

    • Author(s)
      Tsujioka, M., Yokoyama, M., Nishino, K., Kuwayama, H., Morio, T., Katoh, M., Urushihara, H., Saito, T., Ochiai, H., Tanaka, Y., Takeuchi, I., Maeda, M.
    • Journal Title

      Dev.Growth & Differ. 43

      Pages: 275-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] FebA : a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum.2001

    • Author(s)
      Morio, T., Yasukawa, H., Urushihara, H., Saito, T., Ochiai, H., Takeuchi, I., Maeda, M., Tanaka, Y.
    • Journal Title

      Biochim.Biophys.Acta 1519

      Pages: 65-69

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A second functional delta-5 fatty acid desaturase in the cellular slime mold Dictyostelium discoideum.2000

    • Author(s)
      Saito, T.
    • Journal Title

      Eur. J. Biochem. 267

      Pages: 1813-1818

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium.2000

    • Author(s)
      Mitra, B.N.
    • Journal Title

      Gene 251

      Pages: 131-139

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium.2000

    • Author(s)
      Maeda, M.
    • Journal Title

      Dev. Biol. 223

      Pages: 114-119

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion.2000

    • Author(s)
      Bogdanovic, A.
    • Journal Title

      J Biol. Chem. 275

      Pages: 36691-36697

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A second functional delta-5 fatty acid desaturase in the cellular slime mold Dictyostelium discoideum.2000

    • Author(s)
      Saito, T., Morio, T., Ochiai, H.
    • Journal Title

      Eur.J.Biochem. 267

      Pages: 1813-1818

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium.2000

    • Author(s)
      Mitra, B.N., Yoshino, R., Morio, T., Yokoyama, M., Maeda, M., Urushihara, H., Tanaka, Y.
    • Journal Title

      Gene 251

      Pages: 131-139

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium.2000

    • Author(s)
      Maeda, M., Kuwayama, H., Yokoyama, M., Nishino, K., Morio, T., Urushihara, H., Katoh, M., Tanaka, Y., Saito, T., Ochiai, H., Takemoto, K., Yasukawa, H., Takeuchi, I.
    • Journal Title

      Dev. Biol. 223

      Pages: 114-119

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A syntaxin 7 homologue is present in Dictyostelium discoideum endosomes and controls their homotypic fusion.2000

    • Author(s)
      Bogdanovic, A., Bruckert, F., Morio, T., Satre, M.
    • Journal Title

      J.Biol.Chem. 275(47)

      Pages: 36691-36697

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dictyostelium transcriptional host cell response upon infection with Legionella.

    • Author(s)
      Farbrother, P., Wagner, C., Na, J., Budi, N.A., Tunggal, B., Morio, T., Urushihara, H., Tanaka, Y., Schleicher, M., Steinert, M., Eichinger, L.
    • Journal Title

      Cell.Microbiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion.

    • Author(s)
      Muramoto, T., Urushihara, H.
    • Journal Title

      Dev.Growth Differ. (in press)

    • NAID

      10018123285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The cDNA Sequencing Project.

    • Author(s)
      Urushihara, H., Morio, T., Tanaka, Y.
    • Journal Title

      Methods in Molecular Biology : Dictyostelium discoideum. (eds. Eichinger, L. andRivero, F.) (Humana Press) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] The cDNA Sequencing Project. In : Methods in Molecular Biology : Dictyostelium discoideum.2006

    • Author(s)
      Urushihara, H.
    • Publisher
      Humana Press(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 「細胞性粘菌のゲノム解析」ゲノミクス・プロテオミクスの新展開〜生物情報の解析と応用〜2004

    • Author(s)
      田仲可昌, 漆原秀子, 森尾貴広
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2004 Annual Research Report
  • [Publications] Urushihara, H. et al.: "Analyses of cDNAs from growth and slug stages of Dictyostelium discoideum"Nucleic Acids Research. 32(5). 1647-1655 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakamoto, H. et al.: "Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during development"Development Growth & Differentiation. 45(5-6). 507-514 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Muramoto, T. et al.: "Construction of a gamete-enriched gene pool and RNAi-mediated functional analysis in Dictyostelium discoideum"Mechanisms of Development. 120. 967-977 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Araki, T. et al.: "A STAT-regulated, stress-induced signalling pathway in Dictyostelium"Journal of Cell Science. 116. 2907-2915 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Maeda, M. et al.: "Changing patterns of gene expression in prestalk cell subtypes of Dictyostelium recognized by in situ hybridization with genes from microarray analyses"Eukaryotic Cell. 2. 638-645 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takeda, K. et al.: "A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium"Gene. 312. 321-333 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mineko MAEDA: "Changing patterns of gene expression in prestalk cell subtypes of Dictyostelium recognized by in situ hybridization with genes from microarray analyses"Eukaryotic Cell. (In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hidekazu KUWAYAMA: "A novel PCR-mediated method for one tube generation of a gene disruption construct"Biotechnology letters. 24. 1307-1312 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nancy Van DRIESSCHE: "A transcriptional profile of multicellular development in Dictyostelium discoideum"Development. 129. 1543-1552 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hideko URUSHIHARA: "Functional genomics in Dictyostelium discoideum"Molecules and Cells. 13. 1-4 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 桑山 秀一: "PCR法による遺伝子破壊コンストラクトの作製法"日本農芸化学会誌. 77. 150-153 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Aleksandra BOGDANOVIC: "Syntaxin 7, syntaxin 8, Vtil and VAMP7(vesicle-associated membrane protein 7)form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum"Biochemical Journal. 368. 29-39 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nancy VAN DRIESSCHE: "A transcriptional profile of multicellular development in Dictyostelium disooideum"Development. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hidekazu KUWAYAMA: "PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors"Nucleic Acids Research. 30. e2 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takahiro MORIO: "FebA : a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum"Biochimica et Biophysica Acta.. 1519. 65-69 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hideko URUSHIHARA: "Functional genomics in Dictyostelium discoideum"Molecules and Cells. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masatsune TSUJIOKA: "Spatial expression patterns of genes involved in cAMP-responses in Dictyostelium development."Development Growth and Differentiation. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hideatei MIZUNO: "ORI-GENE : A tool for gene classification based on the evolutionary tree."Bioinformatics. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Biswa Nath MITRA: "Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium."Gene. 251. 131-139 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mineko MAEDA: "Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium."Developmental Biology. 223. 114-119 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2018-03-28  

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