| Project/Area Number |
12206006
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto University |
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Principal Investigator |
NISHIOKA Takaaki Kyoto University, Graduate School of Agriculture, Professor, 農学研究科, 教授 (80026559)
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| Co-Investigator(Kenkyū-buntansha) |
TERABE Shigeru University of Hyoto, Graduate School of Material Science, Professor, 物質理学研究科, 教授 (50115888)
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| Project Period (FY) |
2000 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥89,200,000 (Direct Cost: ¥89,200,000)
Fiscal Year 2004: ¥21,600,000 (Direct Cost: ¥21,600,000)
Fiscal Year 2003: ¥21,600,000 (Direct Cost: ¥21,600,000)
Fiscal Year 2002: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2001: ¥25,000,000 (Direct Cost: ¥25,000,000)
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| Keywords | metabolomics / metabolism / capillary electrophoresis / metabolite profile / homeostasis / CE / MS / ESI-IontrapMS / ESI-MS / TOFMS / 2次元分離 / LC / メタボローム解析 / 枯草菌 / 医療診断 / 創薬 / キャピラリー内濃縮 / pHジャンクション / ハイスループット化学分析 / レーザー励起蛍光 / アミノ酸 / 有機酸 / ゲノム / ネットワーク / 代謝物 |
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| Research Abstract |
Most of the metabolites in living cells or tissues are polar and ionic substances that are not suitable for chemical analysis by using conventional methods such as GC-and LC-MS. We have successfully developed capillary electrophoresis coupled with mass spectrometry (CE-MS) as a method of metabolomics. We applied the method to analyze the metabolic intermediates of the central energy metabolism of the Bacillus subtilis cells and compared the metabolite profiles that schematically show the intercellular amount of the metabolites on the central metabolic pathways of the cells cultured under various environmental and genetic perturbations. The metabolite profiles were globally same when the rates of the cell division were the same under the different perturbations. This suggests that B. subtilis has a metabolite profile that is optimized for its cell division and that it maintain the profile against the environmental and genetic perturbations. At the same times when we analyzed the metabolites, we measured the gene expressions by using the DNA microarray of B. subtilis. Gene expression data were overlaid on the metabolite profiles. When perturbations were not so different from the control experiment, amount of the expressions of several enzyme genes were regulated. On the others where perturbations were quite different, B. subtilis changed not only gene expressions but also metabolic pathways; it used a quite different metabolic pathways from those of the control experiment. These results suggests that the metabolite profile is quantitatively and qualitatively regulated; this must be a way of "homeostasis". It is most likely that each living species has its own metabolite profile for cell division.
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