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Protein-protein interaction network supporting growth and differentiation of Bacillus subtilis

Research Project

Project/Area Number 12206007
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNara Institute of Science and Technology

Principal Investigator

OGASAWARA Naotake  Nara Institute of Science and Technology, Graduate School of Information Science, Professor, 情報科学研究科, 教授 (10110553)

Co-Investigator(Kenkyū-buntansha) KANATA Shigehiko  Nara Institute of Science and Technology, Graduate School of Information Science, Professor, 情報科学研究科, 教授 (90224584)
KAWAMURA Fujio  Rikkyo University, Faculty of Science, Professor, 理学部, 教授 (10126039)
YOSHIKAWA Hirofumi  Tokyo University of Agriculture, Department of Bioscience, Professor, 応用生物科学部, 教授 (50175676)
WATABE Kazuhito  Setsunan Univaersity, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (20107105)
SATO Thutomu  Tokyo University of Agriculture and Technology, Graduate School of Agriculture, Associate Professor, 共生科学技術研究部, 助教授 (70215812)
小林 和夫  奈良先端科学技術大学院大学, 情報科学研究科, 助手 (70324978)
笠原 康裕  奈良先端科学技術大学院大学, 情報科学研究科, 助手 (20273849)
守家 成紀 (守夜 成紀)  奈良先端科学技術大学院大学, 情報科学研究科, 助教授 (40191051)
Project Period (FY) 2000 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥135,700,000 (Direct Cost: ¥135,700,000)
Fiscal Year 2004: ¥35,200,000 (Direct Cost: ¥35,200,000)
Fiscal Year 2003: ¥35,000,000 (Direct Cost: ¥35,000,000)
Fiscal Year 2002: ¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 2001: ¥35,500,000 (Direct Cost: ¥35,500,000)
KeywordsBacillus subtilis / Genome / Essential gene / Protein-protein interaction / Spore / 枯草菌 / リボゾーム / 蛋白質相互作用 / 蛋白質複合体 / 質量分析法 / 酵母2ハイブリッドシステム / 変異株バンク / 酵母2ハイブリッド系 / GFP / ゲノム解析
Research Abstract

1) We newly constructed about 300 mutants of the genes identified by genome sequencing, to complete the international mutant construction project, and reported that 271 genes are essential for the B. subtilis growth at 37℃ in LB medium.
2) Among essential genes without known function, we have found that yacA encodes an RNA-modifying enzyme responsible for lysidine formation and yneS, together with plsX, is essential for the first step of phospholipids biosynthesis. In addition, one of the essential GTP binding proteins, YlqF, has been demonstrated to participate in the last step of 50S ribosome subunit assembly.
3) We have attempted to construct knock out mutant of each of 57 ribosome protein genes, and found that 22 are dispensable for the growth.
4) We have systematically analyzed the synthetic lethality of double knockout of paralogous gene pairs, and found that the polA-yacP double mutant is lethal due to the defect in removal of primer RNA of Okazaki fragments.
5) By combination of proteome, transcriptome and genetic analysis, in addition to 119 genes previously reported, we have newly identified 175 genes that are expressed specifically during sporulation, and determined sigma factors responsible for their expression. We have also analyzed the localization of GFP fusions of about 90 spore proteins, and found at least 10 different the localization patterns.
6) We have attempted the analysis of protein-protein interaction network by using yeast two-hybrid analysis and mass spectroscopic analysis of in vivo protein complex, and identified several interesting interactions; anti-sigma proteins regulating activities of ECF sigma factors, Rap proteins regulating ComK responsible for competence development, YheIH involved in regulation of phosphor-relay inducing initiation of sporulation, YlqF interacting with FtsZ and involved in cell division, and YabA interacting with DnaA and DnaA and regulating initiation of genome replication.

Report

(6 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (102 results)

All 2006 2005 2004 2003 2002 2001 Other

All Journal Article (76 results) Publications (26 results)

  • [Journal Article] Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus subtilis2006

    • Author(s)
      Noirot-Gros, M. F.
    • Journal Title

      Proc Natl Acad Sci USA 103

      Pages: 2368-2373

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The GTP-binding protein, YlqF, participates in the late step of 50S ribosomal subunit assembly in Bacillus subtilis2006

    • Author(s)
      Matsuo, Y.
    • Journal Title

      J Biol Chem 281

      Pages: 8110-8117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bacillus subtilis EzrA and FtsL synergistically regulate FtsZ ring dynamics during cell division2006

    • Author(s)
      Kawai, Y.
    • Journal Title

      Microbiology 152

      Pages: 1129-1141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The putative ABC transporter YheH/YheI is involved in the signaling pathway that activates KinA during sporulation initiation2006

    • Author(s)
      Fukushima, S.
    • Journal Title

      FEMS Microbiol Lett 256

      Pages: 90-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus subtilis.2006

    • Author(s)
      Noirot-Gros, M.F.; Velten, M., Yoshimura, M., McGovern, S., Morimoto, T., Ehrlich, S.D., Ogasawara, N., Polard, P., Noirot, P.
    • Journal Title

      Proc Nati Acad Sci U S A. 103

      Pages: 2368-2373

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The GTP-binding protein, YlqF, participates in the late step of 50S ribosomal subunit assembly in Bacillus subtilis.2006

    • Author(s)
      Matsuo, Y., Morimoto, T., Kuwano, M., Loh, P.C., Oshima, T., Ogasawara, N.
    • Journal Title

      J Biol Chem 281

      Pages: 8110-8117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bacillus subtilis EzrA and FtsL synergistically regulate FtsZ ring dynamics during cell division.2006

    • Author(s)
      Kawai, Y., Ogasawara, N.
    • Journal Title

      Microbiology 152

      Pages: 1129-1141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The putative ABC transporter YheH/YheI is involved in the signaling pathway that activates KinA during sporulation initiation.2006

    • Author(s)
      Fukushima, S., Yoshimura, M., Chibazakura, T., Sato, T., Yoshikawa, H.
    • Journal Title

      FEMS Microbiol Lett 256

      Pages: 90-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems2005

    • Author(s)
      Bongiorni, C.
    • Journal Title

      J Bacteriol 187

      Pages: 4353-4361

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore2005

    • Author(s)
      Kuwana R.
    • Journal Title

      FEMS Microbiol Lett 242

      Pages: 51-57

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.2005

    • Author(s)
      Bongiorni, C., Ishikawa, S., Stephenson, S., Ogasawara, N., Perego, M.
    • Journal Title

      J Bacteriol 187

      Pages: 4353-4361

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore.2005

    • Author(s)
      Kuwana R., Okumura T., Takamatsu H., Watabe K.
    • Journal Title

      FEMS Microbiol Lett 242

      Pages: 51-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The, ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore2005

    • Author(s)
      Kuwana, R.
    • Journal Title

      FEMS Microbiol.Lett. 242・1

      Pages: 51-57

    • Related Report
      2004 Annual Research Report
  • [Journal Article] DBTBS : database of transcriptional regulation in Bacillus subtilis and its contribution to comparative genomics2004

    • Author(s)
      Makita, Y.
    • Journal Title

      Nucleic Acids Res 32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome2004

    • Author(s)
      Nanamiya, H.
    • Journal Title

      Mol Microbiol 52

      Pages: 273-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis2004

    • Author(s)
      Kuwana, R.
    • Journal Title

      Microbiology 150

      Pages: 163-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] spoIVH (ykvV), a requisite cortex formation gene, is expressed in both sporulating compartments of Bacillus subtilis2004

    • Author(s)
      Imamura, D.
    • Journal Title

      J Bacteriol 186

      Pages: 450-5459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses2004

    • Author(s)
      Serizawa, M.
    • Journal Title

      Gene 329

      Pages: 125-136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors2004

    • Author(s)
      Yoshimura, M.
    • Journal Title

      Microbiology 150

      Pages: 591-599

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis2004

    • Author(s)
      Fukushima, T.
    • Journal Title

      J Biochem 136

      Pages: 283-291

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome.2004

    • Author(s)
      Nanamiya, H., Akanuma, G., Natori, Y., Murayama, R., Kosono, S., Kudo, T., Kobayashi, K., Ogasawara, N., Park, S.M., Ochi, K., Kawamura, F.
    • Journal Title

      Mol Microbiol 52

      Pages: 273-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] spoIVH (ykvV), a requisite cortex formation gene, is expressed in both sporulating compartments of Bacillus subtilis.2004

    • Author(s)
      Imamura, D., Kobayashi, K., Sekiguchi, J., Ogasawara, N., Takeuchi, M., Sato, T.
    • Journal Title

      J Bacteriol 186

      Pages: 5450-5459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis.2004

    • Author(s)
      Kuwana, R., Ikejiri, H., Yamamura, S., Takamatsu, H., Watabe, K.
    • Journal Title

      Microbiology 150

      Pages: 163-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors.2004

    • Author(s)
      Yoshimura, M., Asai, K., Sadaie, Y., Yoshikawa, H.
    • Journal Title

      Microbiology 150

      Pages: 591-599

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DBTBS : database of transcriptional regulation in Bacillus subtilis and its contribution to comparative genomics.2004

    • Author(s)
      Makita, Y., Nakao, M., Ogasawara, N., Nakai, K.
    • Journal Title

      Nucleic Acids Res 32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.2004

    • Author(s)
      Serizawa, M., Yamamoto, H., Yamaguchi, H., Fujita, Y., Kobayashi, K., Ogasawara, N., Sekiguchi, J.
    • Journal Title

      Gene 329

      Pages: 125-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.2004

    • Author(s)
      Fukushima, T., Tanabe, T., Yamamoto, H., Hosoya, S., Sato, T., Yoshikawa, H., Sekiguchi, J.
    • Journal Title

      J Biochem. 136

      Pages: 283-291

    • NAID

      10016201890

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Zinc is a key factor in controlling alteration of two types of L31 protein in the Bacillus subtilis ribosome2004

    • Author(s)
      Nanamiya, H.
    • Journal Title

      Mol.Microbiol. 52・1

      Pages: 273-283

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N terminal regions of their potential anti-sigma factors2004

    • Author(s)
      Yoshimura, M.
    • Journal Title

      Microbiology 150・3

      Pages: 591-599

    • Related Report
      2004 Annual Research Report
  • [Journal Article] spoIVH (ykvV), a requisite cortex formation gene, expresses in both sporulating compartments of Bacillus subtilis2004

    • Author(s)
      Imamura, D
    • Journal Title

      J.Bacteriol. 186・16

      Pages: 5450-5459

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Essential Bacillus subtilis genes2003

    • Author(s)
      Kobayashi, K.
    • Journal Title

      Proc Natl Acad Sci USA 100

      Pages: 4678-4683

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA2003

    • Author(s)
      Soma, A.
    • Journal Title

      Mol Cell 12

      Pages: 689-698

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis2003

    • Author(s)
      Kawai, Y.
    • Journal Title

      Mol Microbiol 47

      Pages: 1113-1122

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The BceRS two-component regulatory system induces expression of the bacitracin transporter, BceAB, in Bacillus subtilis2003

    • Author(s)
      Ohki, R.
    • Journal Title

      Mol Microbiol 49

      Pages: 1135-1144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis2003

    • Author(s)
      Ogura, M
    • Journal Title

      Mol Microbiol 49

      Pages: 1685-1697

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium2003

    • Author(s)
      Tanaka, K.
    • Journal Title

      Microbiology 149

      Pages: 2317-2329

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bacillus subtilis diacylglycerol kinase (DgkA) enhances efficient sporulation2003

    • Author(s)
      Amiteye, S.
    • Journal Title

      J Bacteriol 185

      Pages: 5306-5309

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ncreasing the ratio of Soj to SpoOJ promotes replication initiation in Bacillus subtilis2003

    • Author(s)
      Ogura, Y.
    • Journal Title

      J Bacteriol 185

      Pages: 6316-6324

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance2003

    • Author(s)
      Kuwana, R.
    • Journal Title

      Microbiology 149

      Pages: 3011-3021

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis2003

    • Author(s)
      Nanamiya, H.
    • Journal Title

      J Biochem (Tokyo) 133

      Pages: 295-302

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transcriptional, functional and cytochemical analyses of the veg gene in Bacillus subtilis2003

    • Author(s)
      Fukushima, T.
    • Journal Title

      J Biochem (Tokyo) 133

      Pages: 475-483

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Essential Bacillus subtilis genes.2003

    • Author(s)
      Kobayashi, K., Ehrlich, S.D., Albertini, A., Amati, G., Andersen, K.K., Arnaud, M., Asai, K., Ashikaga, S., Aymerich, S., Bessieres, P., et al.
    • Journal Title

      Proc Natl Acad Sci U S A 100

      Pages: 4678-4683

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA.2003

    • Author(s)
      Soma, A., Ikeuchi, Y., Kanemasa, S., Kobayashi, K., Ogasawara, N., Ote, T., Kato, J., Watanabe, K., Sekine, Y., Suzuki, T.
    • Journal Title

      Mol Cell 12

      Pages: 689-698

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis.2003

    • Author(s)
      Kawai, Y., Moriya, S., Ogasawara, N.
    • Journal Title

      Mol Microbiol 47

      Pages: 1113-1122

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The BceRS two-component regulatory system induces expression of the bacitracin transporter, BceAB, in Bacillus subtilis.2003

    • Author(s)
      Ohki, R., Giyanto, Tateno, K., Masuyama, W., Moriya, S., Kobayashi, K., Ogasawara, N.
    • Journal Title

      Mol Microbiol 49

      Pages: 1135-1144

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis.2003

    • Author(s)
      Ogura, M., Shimane, K., Asai, K., Ogasawara, N., Tanaka, T.
    • Journal Title

      Mol Microbiol 49

      Pages: 1685-1697

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bacillus subtilis diacylglycerol kinase (DgkA) enhances efficient sporulation.2003

    • Author(s)
      Amiteye, S., Kobayashi, K., Imamura, D., Hosoya, S., Ogasawara, N., Sato, T.
    • Journal Title

      J Bacteriol 185

      Pages: 5306-5309

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increasing the ratio of Soj to SpoOJ promotes replication initiation in Bacillus subtilis.2003

    • Author(s)
      Ogura, Y., Ogasawara, N., Harry, E.J., Moriya, S.
    • Journal Title

      J Bacteriol 185

      Pages: 6316-6324

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.2003

    • Author(s)
      Tanaka, K., Kobayashi, K., Ogasawara, N.
    • Journal Title

      Microbiology 149

      Pages: 2317-2329

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance.2003

    • Author(s)
      Kuwana, R., Yamamura, S., Ikejiri, H., Kobayashi, K., Ogasawara, N., Asai, K., Sadaie, Y., Takamatsu, H., Watabe, K.
    • Journal Title

      Microbiology 149

      Pages: 3011-3021

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis.2003

    • Author(s)
      Nanamiya, H., Shiomi, E., Ogura, M., Tanaka, T., Asai, K., Kawamura, F.
    • Journal Title

      J Biochem (Tokyo) 133

      Pages: 295-302

    • NAID

      10012053276

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transcriptional, functional and cytochemical analyses of the veg gene in Bacillus subtilis.2003

    • Author(s)
      Fukushima, T., Ishikawa, S., Yamamoto, H., Ogasawara, N., Sekiguchi, J.
    • Journal Title

      J Biochem (Tokyo) 133

      Pages: 475-483

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members ScpA and ScpB2002

    • Author(s)
      Soppa, J.
    • Journal Title

      Mol Microbiol 45

      Pages: 59-71

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of the Bacillus subtilis spoIIIJ gene and its paralogue gene, yqjG2002

    • Author(s)
      Murakami, T.
    • Journal Title

      J Bacteriol 184

      Pages: 1998-2004

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK2002

    • Author(s)
      Ogura, M.
    • Journal Title

      J Bacteriol 184

      Pages: 2344-2351

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutation in yaaT leads to significant inhibition of phosphorelay during sporulation in Bacillus subtilis2002

    • Author(s)
      Hosoya, S.
    • Journal Title

      J Bacteriol 184

      Pages: 5545-5553

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis2002

    • Author(s)
      Morimoto, T.
    • Journal Title

      Microbiology 148

      Pages: 3539-3352

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteomics characterization of novel spore proteins of Bacillus subtilis2002

    • Author(s)
      Kuwana, R.
    • Journal Title

      Microbiology 148

      Pages: 3971-3982

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ2002

    • Author(s)
      Hosoya, S.
    • Journal Title

      FEMS Microbiol. Lett 210

      Pages: 193-199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DnaK chaperone machine and trigger factor are only partially required for normal growth of Bacillus subtilis2002

    • Author(s)
      Reyes, D. Y.
    • Journal Title

      Biosci Biotechnol Biochem 66

      Pages: 1583-1586

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] (2002). Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members ScpA and ScpB.2002

    • Author(s)
      Soppa, J., Kobayashi, K., Noirot-Gros, M.F., Oesterhelt, D., Ehrlich, S.D., Dervyn, E., Ogasawara, N., Moriya, S.
    • Journal Title

      Mol Microbiol 45

      Pages: 59-71

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of the Bacillus subtilis spoIIIJ gene and its paralogue gene, yqjG.2002

    • Author(s)
      Murakami, T., Haga, K., Takeuchi, M., Sato, T.
    • Journal Title

      J Bacteriol 184

      Pages: 1998-2004

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.2002

    • Author(s)
      Ogura, M., Yamaguchi, H., Kobayashi, K., Ogasawara, N., Fujita, Y., Tanaka, T.
    • Journal Title

      J Bacteriol 184

      Pages: 2344-2351

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutation in yaaT leads to significant inhibition of phosphorelay during sporulation in Bacillus subtilis.2002

    • Author(s)
      Hosoya, S., Asai, K., Ogasawara, N., Takeuchi, M., Sato, T.
    • Journal Title

      J Bacteriol 184

      Pages: 5545-5553

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.2002

    • Author(s)
      Morimoto, T., Loh, P.C., Hirai, T., Asai, K., Kobayashi, K., Moriya, S., Ogasawara, N.
    • Journal Title

      Microbiology 148

      Pages: 3539-3352

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proteomics characterization of novel spore proteins of Bacillus subtilis.2002

    • Author(s)
      Kuwana, R., Kasahara, Y., Fujibayashi, M., Takamatsu, H., Ogasawara, N., Watabe, K.
    • Journal Title

      Microbiology 148

      Pages: 3971-3982

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.2002

    • Author(s)
      Hosoya, S., Yamane, K., Takeuchi, M., Sato, T.
    • Journal Title

      FEMS Microbiol Lett 210

      Pages: 193-199

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DnaK chaperone machine and trigger factor are only partially required for normal growth of Bacillus subtilis.2002

    • Author(s)
      Reyes, D.Y., Yoshikawa, H.
    • Journal Title

      Biosci Biotechnol Biochem 66

      Pages: 1583-1586

    • NAID

      110002693868

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis.2001

    • Author(s)
      Yoshida, K
    • Journal Title

      Nucleic Acids Res 29

      Pages: 683-692

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication2001

    • Author(s)
      Ishigo-Oka, D.
    • Journal Title

      J Bacteriol 183

      Pages: 2148-2150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems2001

    • Author(s)
      Kobayashi, K.
    • Journal Title

      J Bacteriol 183

      Pages: 7365-7370

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A putative ATP-binding cassette transporter YbdA involved in sporulation of Bacillus subtilis2001

    • Author(s)
      Isezaki, M
    • Journal Title

      FEMS Microbiol Lett 204

      Pages: 239-245

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis.2001

    • Author(s)
      Yoshida, K., Kobayashi, K., Miwa, Y., Kang, C., Matsunaga, M., Yamaguchi, H., Tojo, S., Yamamoto, M., Nishi, R., Ogasawara, N., Nakayama, T., Fujita, Y.
    • Journal Title

      Nucleic Acids Res 29

      Pages: 683-692

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication.2001

    • Author(s)
      Ishigo-Oka, D., Ogasawara, N., Moriya, S.
    • Journal Title

      J Bacteriol 183

      Pages: 2148-2150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems.2001

    • Author(s)
      Kobayashi, K., Ogura, M., Yamaguchi, H., Yoshida, K., Ogasawara, N., Tanaka, T., Fujita, Y.
    • Journal Title

      J Bacteriol 183

      Pages: 7365-7370

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A putative ATP-binding cassette transporter YbdA involved in sporulation of Bacillus subtilis.2001

    • Author(s)
      Isezaki, M., Hosoya, S., Takeuchi, M., Sato, T.
    • Journal Title

      FEMS Microbiol Lett 204

      Pages: 239-245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kawai, Y.: "Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis"Mol Microbial. 47. 1113-1122 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nanamiya, H.: "Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis"J Biochem (Tokyo). 133. 295-302 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fukushima, T.: "Transcriptional, functional and cytochemical analyses of the veg gene in Bacillus subtilis"J Biochem (Tokyo). 133. 475-483 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kobayashi, K.: "Essential Bacillus subtilis genes"Proc Natl Acad Sci USA. 100. 4678-4683 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohki, R.: "The BeeRS two-component regulatory system induces expression of the bacitracin transporter, BceAB, in Bacillus subtilis"Mol Microbiol. 49. 1135-1144 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Amiteye, S.: "Bacillus subtilis diacylglycerol kinase (DgkA) enhances efficient sporulation"J Bacteriol. 185. 5306-5309 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ogura, M.: "Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis"Mol Microbiol. 49. 1685-1697 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka, K.: "The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium"Microbiology. 149. 2317-2329 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuwana, R.: "Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance"Microbiology. 149. 3011-3021 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohashi, Y.: "Expression profiling of translation-associated genes in sporulating Bacillus subtilis and consequence of sporulation by gene inactivation"Biosci Biotechnol Biochem. 67. 2245-2253 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kuwana, R.: "Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis"Microbiology. 150. 163-170 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshimura, M.: "Interaction of Bacillus subtilis extracytoplasmic function (ECF) sigma factors with the N-terminal regions of their potential anti-sigma factors"Microbiology. 150. 591-599 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Soppa, J.: "Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members SpA and ScpB"Mol Microbiol. 45・1. 59-71 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Reyes, D. Y.: "DnaK Chaperone Machine and trigger factor are only partially required for normal growth of Bacillus subtilis"Biosci Biotech Biochem. 66・7. 1583-1586 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Morimoto, T.: "Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis"Microbiology. 148・11. 3539-3552 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawai, Y.: "Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis"Mol Microbiol. 47・4. 1113-1122 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nanamiya, H.: "Involvement of ClpX Protein in the Post-Transcriptional Regulation of Competence Specific Transcription Factor, ComK Protein, of Bacillus subtilis"J Biochemistry. 133・3. 295-302 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kobayashi, K.: "Essential Bacillus subtilis genes"Proc Natl Acad Sci USA. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshida, K.: "Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis"Nucleic Acids Res.. 29. 683-692 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishigo-Oka, D.: "DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication"J. Bacteriol.. 183. 2148-2150 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishigo-oka,D.: "DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication."J.Bacteriol.. 183・6. 2148-2150 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukuchi,K.: "The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis."Microbiology. 146・7. 1573-1583 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Imai,Y.: "Subcellular localization of Dna-initiation proteins of Bacillus subtilis : evidence that chromosome replication begins at either edge of nucleoids."Mol.Microbiol.. 36・5. 1037-1048 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakano,M.M.: "Mutations conferring amino acid residue substitutions in the carboxyl-terminal domain of RNA polymerase αcan suppress clpX and clpP with respect to developmentally regulated transcription in Bacillus subtilis."Mol.Microbiol.. 37・4. 869-884 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kobayashi,H.: "Genetic analysis of SecA-SecY interaction required for spore development in Bacillus subtilis."FEMS Microbiol.Lett.. 184・2. 285-289 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nanamiya,H.: "Suppression of temperature-sensitive sporulation mutation in the Bacillus subtilis sigA gene by rpoB mutation."FEMS Microbiol.Lett.. 192・2. 237-241 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2018-03-28  

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