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Quantitative microbiological technology for advanced management for water environment

Research Project

Project/Area Number 12305032
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionThe University of Tokyo

Principal Investigator

OHGAKI Shinichiro  Graduate School of Engineering, The University of Tokyo, Professor, 大学院・工学系研究科, 教授 (20005549)

Co-Investigator(Kenkyū-buntansha) HARADA Hideaki  Nagaoka University of Technology, Graduate School oi engineering, Professor, 大学院・工学研究科, 教授 (70134971)
FUJITA Masanori  Osaka University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (70029289)
OMURA Tatsuo  Tohoku University, Graduate School of Engineering, Professor, 大学院・工学系研究科, 教授 (30111248)
ENDO Ginro  Tohoku Gakuin University, Faculty of Engineering, Professor, 工学部, 教授 (80194033)
TSUNO Hiroshi  Kyoto University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (40026315)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥32,840,000 (Direct Cost: ¥29,300,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2001: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2000: ¥17,500,000 (Direct Cost: ¥17,500,000)
KeywordsWater microbiology / quantification / gene technology / PCR / FISH / antibiotics / VIRUS / concentration / 微生物 / 水環境 / 上下水道 / 遺伝子 / 酵素
Research Abstract

There had been a great progress in genetic engineering and analytical methods for water chemistry in the last decade of 20th century. However, these techniques were not directly applied to the field of the environmental engineering, because it includes such difficulty in understanding the phenomena that low concentration and complicated microbial community while many other compounds are interfere the analytical methods. In this study, in order to utilize the emerging technology for environmental engineering for water management, various efforts were paid for quantification of microbes for the purpose of elucidation of complex microbial community and the fates of pathogenic microbes in water environment.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] katayama H., Shimasaki A., Ohgaki S.: "Development of a Virus Concentration Method and Its Application to Detection of Enterovirus and Norwalk Virus from Coastal Sea Water"Applied and Environmental Microbiology. 68:3. 1033-1039 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katayama H., M.Nakamura, S.Ohgaki: "PCR determination of inactivated RNA coliphage Qβ"Advances in Water and Wastewater Treatment Technology …Molecular technology, nutrient removal. sludge reduction, and environmental health. 1. 101-108 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sei K., Nakao M., Mori K., Ike M., Kohno T., Fujita M: "Design of PCR primers and a gene probe for extensive detection of poly(3-hydroxybutyrate)(PHB)-degrading bacteria possessing fibronectin type III linker type-PHB depolymerases"Applied Microbiology and Biotechnology. 55. 801-806 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D.Sano, K.Fukushi, K.Yano.Y.Yoshida, T.Omura: "Enhanced Virus Recovery from Municipal Sewage Sludge with a Combination of Enzyme and Cation Exchange Resin"Water. Science and Technology. 43(2). 75-82 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Watanabe, D.Sano, T.Omura: "Risk Evaluation for Pathogenic Bacteria and Viruses in the Compost of Sewage Sludge"Proceedings of 1st International Water Association(IWA)Asia-Pacific Regional Conference. 1. 729-734 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Sekiguchi, H.Takahashi, Y.Kamagata, A.Ohashi, H.Harada: "In situ Detection, Isolation and Physiological Properties of a Thin Filamentous Microoganism Abundant in Methanogenic Granular Sludges : a Novel Isolate affiliated with a Clone Cluster, the Green Non Sulfur Bacteria Subdivision 1"Appl. Environ. Microbiol.. 67・12. 5740-5749 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Alam, M. Z. B., Ohtaki, M., Furumai, H., Ohgaki, S.: "Direct and indirect inactivation of Microcystis aeruginosa by UV-radiation"Water Research. Vol. 35, No. 4. 1008-1014 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katayama, H., Shimasaki, A., Ohgaki, S.: "Development of a Virus Concentration Method and Its Application to Detection of Enterovirus and Norwalk Virus from Coastal Seawater"Applied and Environmental Microbiology. 1033-1039 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katayama, H., Kuyama, T., Ohgaki, S.: "Daily and Seasonal Profits of Coliforms and F-specific RNA Phase in Indigenous Oysters and Marine Water in Tokyo Bay"Proc. of 1st IWA Asia-Pacific Regional Conference, Fukuoka. 12-15 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Alam, M.Z.B., Luxmy, B.S., Katayama, H., Ohgaki, S.: "Detecting the Viability of Particle Associated Bacteria after UV-radiation: A Molecular Biological Approach"Proc. of First Asian Pacific Regional Conference of IWA, Fukuoka. 661-666 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sei, K., Asano, K., Tateishi, N., Mori, K., Ike, M., Kohno, T., Fujita, M.: "Development of Simple Methods of DNA Extraction from Environmental Sample for Monitoring Microbial Community Based on PCR"Japanese Jounal of Water Treatment Biology. Vol. 36, No. 4. 193-204 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sei, N., Nakano, M., Mori, K., Ike, K., Kohno, T., Fujita, M.: "Design of FOR Promers and a Gene Probe for Extensive Detection of Poly(3-hydroxybutyrate) (PHB)-degrading Bacteria Possessing Fibronectin Type III Linker Type-PHB Depolymerases"Appl. Microbiol. Biotechnol.. 801-806 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sano, D., Fukushi, K., Yano, K., Yoshida, Y., Omura, T.: "Enhanced Virus Recovery from Municipal Sewage Sluge with a Combination of Enzyme and Cation Exchange Resin"Water Science and Technology. Vol. 43, No. 2. 75-82 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe, T., Sano, D., Omura, T.: "Risk Evalation for Pathogenic Bacteria and Viruses in the Compost of Sewage Sludge"Proc. of 1st International Water Association (IWA) Asia-Pacific Regional Conference. 729-734 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Imachi, H., Sekiguchi, Y., Kamagata, Y., Ohashi, A., Harada, H.: "Cultivation and In Situ Detection of a Thermophilic Bacterium Capable of Oxidizing Propionate in Syntrophic Association with Hydrogenotropnic Methanogens in a Thermophilic Methanogenie Granular Sludge"Applied and Environmental Microbiology. 3608-3615 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sekiguchi, Takahashi, H., Y., Kamagata, Y, Ohashi, A., Harada, H.: "In Situ Detection, Isolation, and Physiological Properties of a Thin Filamentous Microorganism Abundant in Methanogenic Granular Sluges: a Novel Isolate Affiliated with a Clone Cluster, the Green Non-Sulfur Bacteria, Subdivision I"Applied and Environmental Microbiology. 5740-5749 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata, A. Hashimoto and M.Z.B. Alam: "Quantitative Risk Assessment of Cryptosporidium in a Watershed, Advances in Water and Wastewater Treatment, Edited by Matsuo, T., Hanaki, K., Satoh, H."Elsevier. 91-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Alam, M. Z. B., Ohgaki, S.: "Evaluation of UV-radiation and its residual effect for algal growth control, Advances in Water and Wastewater Treatment, Edited by Matsuo, T., Hanaki, K., Satoh, H."Elsevier. 109-117 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katayama H., M. Nakamura and S. Ohgaki: "PCR determination of inactivated RNA colipnage Qβ, Advances in Water and Wastewater Treatment, Edited by Matsuo, T., Hanaki, K., Satoh, H."Elsevier. 101-108 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katayama H., Shimasaki A., Ohgaki S.: "Development of a Virus Concentration Method and Its Application to Detection of Enterovirus and Norwalk Virus from Coastal Sea Water"Applied and Environmental Microbiology. 68・3. 1033-1039 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Katayama H., M. Nakamura, S. Ohgaki: "PCR determination of inactivated RNA coliphage Qβ"Advances in Water and Wastewater Treatment Technology Molecular technology, nutrient removal, sludge reduction, and environmental health. 1. 101-108 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sei K., Nakao M., Mori K., Ike M., Kohno T., Fujita M.: "Design of PCR primers and a gene probe for extensive detection of poly(3-hydroxybutyrate) (PHB)-degrading bacteria possessing fibronectin type III linker type-PHB depolymerases"Applied Microbiology and Biotechnology. 55. 801-806 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] D. Sano, K. Fukushi, K. Yano, Y. Yoshida, T. Omura: "Enhanced Virus Recovery from Municipal Sewage Sludge with a Combination of Enzyme and Cation Exchange Resin"Water. Science and Technology. 43・2. 75-82 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T. Watanabe, D. Sano, T. Omura: "Risk Evaluation for Pathogenic Bacteria and Viruses in the Compost of Sewage Sludge"Proceedings of 1st International Water Association (IWA) Asia-Pacific Regional Conference. 1. 729-734 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y. Sekiguchi, H. Takahashi, Y. Kamagata, A. Ohashi, H. Harada: "In situ Detection, Isolation and Physiological Properties of a Thin Filamentous Microoganism Abundant in Methanogenic Granular Sludges : a Novel Isolate affiliated with a Clone Cluster, the Green Non Sulfur Bacteria Subdivision 1"Appl. Environ. Microbiol.. 67・12. 5740-5749 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] D.Sano,K.Fukushi,K.Yano,Y.Yoshida and T,Omura: "Enhanced Virus Recovery from Municipal Sewage Sludge with Combination of Enzyme and Cation Exchange Resin"Water.Science and Technology. 43(2). 75-82 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M asaru Narita,Chei-Chen Huang,Takuya Koizumi,Takeshi Yamagata,Ginro Endo: "Identification and characterization of anaerobic mercury-resistant bacteria isolated from mercury-polluted sediment."Water Science and Technology. Vol.42 No.3-4. 109-114 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Tagawa,K.Syutsubo,Y.Sekiguchi,A.Ohashi and H.Harada: "Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization."Wat.Sc.& Tech.. vol.42 No.3-4. 77-82 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shutsubo K.,Sinthurat N.,Ohashi A.& Harada H.: "Population Dynamics of Anaerobic Microbial Consortia in Thermophilic Granular Sludge in Response to Feed Composition Change"Wat.Sc.& Tech. vol.43 No.1. 59-66 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Imachi,H.,Sekiguchi T.,Kamagata Y.,Ohashi A.and Harada H.: "Cultivation and Situ Detection of a Thermophilic Bacterium.Capable of Oxidizing Propionate in Syntr ophic Association with Hydrogenotrophic Methanogens in a Thermophilic Methanogenic Granular Sludge"Appl.Environ.Microbiol.. vol.66. 3608-3615 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kazunari Sei,Ken-ichiro Asano,Naohiro Tateishi,Kazuhiro Mori,Michihiko Ike,Tetsuro Kohno,and Masanori Fujita: "Development of Simple Methods of DNA Extraction from Environmental Samples for Monitoring Microbial Community Based on PCR"Japanese Journal of Water Treatment Biology. Vol.36,No.4,. 193-204 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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