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Modecular design of artificial glycoconjugate polymers for developing biotedrnology

Research Project

Project/Area Number 12305059
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 高分子合成
Research InstitutionNagoya University

Principal Investigator

KABAYASHI Kazukiyo  Nagoya U. Engineering. Prof., 工学研究科, 教授 (10023483)

Co-Investigator(Kenkyū-buntansha) MIURA Yoshiko  Nagoya U. Engineering. Associate Prof., 工学研究科, 助手 (00335069)
NISHIDA Yoshihiro  Nagoya U. Engineering. Associate Prof., 工学研究科, 助教授 (80183896)
松浦 和則  名古屋大学, 工学研究科, 助手 (60283389)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2001: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
KeywordsCarbohydrate / Biomaterials / Polymer / Molecular Recognition / DNA / フラーレン
Research Abstract

A complex of pTRI-β-actin plasmid DNA-lactose conjugate with β-galactose-specific RCA_<120) lectin is proposed as a functional mimic of the chromatin structure comprising DNA and histones : the in vitro transcription of DNA in the conjugate with T7 RNA polymerase was repressed in the presence of RCA_<120> and then the transcription ability was recovered by adding lactose or a lactose-carrying polymer to the repression system. The complex can be regarded as an artificial regulation system of transcription.
In conclusion, we have demonstrated the first successful regulation of gene expression with an artificial recognition system using a combination of a plasmid-canpohydtate conjugate with lectin. The concept proposed in this paper has possibility to be extended to various ligand-receptor systems and will open up new dimensions in biomedical researches.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] K.Matsuura: "Construction of Glyco-clusters by Self-ortganization"J. Am. Chem. Soc. 123. 357-358 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Akasaka, K.Matsuura: "Transformation from Block-type to Graft-type"Bioconjugate Chem. 12. 776-785 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Goto, K.Kobayashi: "Micellar behavior of sugar-carrying polystyrene"Macromol. Chem. Phys. 202. 1161-1165 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Nishida, H.Uzawa: "A facile synthetic approach to L-and P-Selectin"Macromolecules. 1. 68-74 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Hasegawa, K.Matsuura: "Multilayer Adsorption of molecular organization"Macromolecules. 33. 2772-2775 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Matsuura, H.Kitakoji: "A Quantitative Estimation of Carbohydrate"J. Am. Chem. Soc. 122. 7406-7407 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松浦和則, 小林一清: "次世代高分子設計"アイシービー. 613 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirofumi Dohi, Yoshihiro Nishida, Yuki Furuta, Hirotaka Uzawa, Shin-ichiro Yokoyama, Hiroshi Mori, Kazukiyo Kobayashi: "Biologic Potential of Galacto-type Trehalose. The First Report of Non-natural Carbohydrate Ligands of Shiga-like Toxins"Org. Lett.. 4. 355-357 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshihiro Nishida, Kideaki Tamakoshi, and Kazukiyo Kobayashi: "A Novel Bovin (β1,4-Galactosyltransferase Reaction with an Acyclic Amino Alcohol to Yield 3-O-β-D-Galactosyl-sn-glycerol Skeleton"Org. Lett.. 3. 1-3 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazunori Matsuura, Miki Hibino, Yoshinao Yamada, and Kazukiyo Kobayashi: "Construction of Glyco-clusters by Self-organization of Site-sepcifically Glycosylated Oligonucleotides and Their Cooperative Amplification of Lectin-recognition"J. Am. Chem. Soc.. 123. 357-358 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teruaki Hasegawa, Takahiro Yonemura, Kazunori Matsuura and Kazukiyo Kobayashi: "Tris-bipyridine ruthenium complex-based glyco-clusters : amplified luminescence and enhanced lectin affinities"Tetrahedron Lett.. 42. 3989-3992 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsukasa Akasaka, Kazunori Matsuura, and Kazukiyo Kobayashi: "Transformation from Block-type to Graft-type of Oligonucleotide-Glycopolymer Conjugates by Self-Organization Assisted with the Half-Sliding Complementary Oligonucleotide and Their Lectin Recognition"Bioconjugate Chem.. 12. 776-785 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirofumi Dohi, Yoshihiro Nishida, Hidehiko Tanaka, and Kazukiyo Kobayashi: "Convenient Use of a Non-Stinky Thio Galactosyl Donor For the Syntheses of Globosyl a(1-4)-Linkages"Synlett. 2001. No. 9. 1446-1448 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Haruhito Kato^a, Arihiro Yashiro^b, Akiko Mizuno^b, Yoshihiro Nishida^b, Kazukiyo Kabayashi^<b*>, and Hisanori Shinohara: "Synthesis and Biological Evaluations of α-D-Mannosyl [60]Fullerenols"Bioorg. Medicinal Chem. Lett.. 11. 2935-2939 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshihiro Nishida, Hirotaka Uzawa, Tadashi Toba, Kenji Sasaki, Hirosato Kondo, Kazukiyo Kobayashi: "A Facile Synthetic Approach to L- and P-Selectin Blockers via Copolymerization of Vinyl Monomers Comprising the Key Interactive Segments of Sialyl Lewis x Mimics"Biomacromolecules. 1. 68-74 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazunori Matsuura, Tsukasa Akasaka, Miki Hibino, and Kazukiyo Kobayashi: "Facile Synthesis of Stable and Lectin-recognizable DNA-Carbohydrate Conjugates via Diazo Coupling"Bioconjugate Chem.. 11. 202-211 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teruaki Hasegawa, Kazunori Matsuura, Katsuhiko Ariga, and Kazukiyo Kobayashi: "Multilayer Adsorption and Molecular Organization of Rigid Cylindrical Glycoconjugate Poly(phenylisocyanide) on Hydrophilic Surfaces"acromolecules. 33. 2772-2775 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hidehiko Tanaka, Yoshihiro Nishida, and Kazukiyo Kobayashi: "A Facile Synthesis of Glycoconjugate Cationic Polymer Carrying 3,6-Branched a-D-Mannosyl Trisaccharide Cluster"J. Carbohydr. Chem.. 19. 413-418 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Matsuura: "Construction of Glyco-clusters by Self-organization"J. Am. Chem. Soc. 123. 357-358 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Akasaka, K.Matsuura: "Transformation from Block-type to Graft-type"Bioconjugate Chem.. 12. 776-785 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Goto, K.Kobayashi: "Micellar behavior of sugar-carrying polystyrene"Macromol. Chem. Phys. 202. 1161-1165 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Nishida, H.Uzawa: "A facile synthetic approach to L- and P-Selection"Biomacromolecules. 1. 68-74 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Hasegawa, K.Matsuura: "Multilayer Adsorption of Molecular Organization"Macromolecular. 33. 2772-2775 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Matsuura, H.Kitakoji: "A Quantitave Estimation of Carbohydrate"J. Am. Chem. Soc. 122. 7406-7407 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松浦和則, 小林一清: "次世代高分子設計"アイシービー. 613 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Matsuura,K.Kobayashi: "Construction of Glyco-clusters by Self-organization of Srte-Specifically Glycosylated Oligonucleotides..."J.Am.Chem.Soc.. 123・2. 357-358 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Nishida,K.Kobayashi: "A Facile Synthetic Approach to L-and P-Selectin Blockers Via Copolymerization of Vinyl...."Biomacromolecules. 1・1. 68-74 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Hasegawa,K.Kobayashi: "Multilayer Adsorption and Molecular Organization of Rigid Cylindrical Glycoconjugate Poly...."Macromolecules. 33・7. 2772-2775 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Tanaka,K.Kobayashi: "A Facile Synthesis of Glycoconjugate Cationic Polymer Carrying 3, 6-Branched a-D-Mannosyl..."J.Carbohydr.Chem.. 19・3. 413-418 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K,Matsuura,K.Kobayashi: "A Quantitive Estimation of Carbohydrate-Carbohydrate Interaction Using Clustered..."J.Am.Chem.Soc. 122. 7406-7407 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Tsuchida,K.Kobayashi: "A Quartz-Crystal Microbalance Study of Adsorption Behaviors of Artificial Glycoconjugate..."Macromol.Chem.Phys.. 201・17. 2245-2250 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小林一清,松浦和則: "次世代糖鎖高分子の合成と機能"アイピーシー. 22 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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