| Project/Area Number |
12306010
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Tohoku University |
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Principal Investigator |
TANIGUCHI Nobuhiko Graduate school of Agricultural Science, Professor, 大学院・農学研究科, 教授 (20036742)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Motohiro Faculty of Agriculture, Ehime University, Associate Professor, 農学部, 助教授 (70335892)
IKEDA Minoru Faculty of Agriculture, Tohoku University, Research Associate, 農学部, 教務職員 (70232204)
NAKAJIMA Masamichi Graduate School of Agricultural Science, Tohoku University, Associate Professor, 大学院・農学研究科, 助教授 (20192221)
鹿野 隆人 東北大学, 大学院・農学研究科, 日本学術振興会特別研究員
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥42,510,000 (Direct Cost: ¥39,600,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2002: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2001: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2000: ¥29,900,000 (Direct Cost: ¥29,900,000)
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| Keywords | Microsatellite DNA / Population Genetics / Genetic Diversity / Artificial Seed / Experimental Fish / Endangered Species |
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| Research Abstract |
Following species were examined for the research experiment of genetic divereity in fishes, Ayu and Masu salmon as representative natural population, Koi carp as representative ornamental fish, Surf fish as representative viviparous fish, Guppy as experimental fish, and Ryukyu-Ayu and Matsukaws as endangered species.
In these species, microsatellite DNA markers were developed to evaluate genetic diversity.
Genetic characteristics such as genetic variability and differences were measured in each species used microsatellite DNA markers.
The reduction of genetic diversity calculated from average heterozygosity in cultured populations were observed as the results of inbreeding.
From the estimation of effective size of population in each cultured population and seed population for release, the degree of emergency could be estimated in the population of the endangered species.
The influence of inbreeding to the population were estimated and methods of remediation was proposed. From the simulation of cross experiment based on the genotype data of parent population, the methods for the genetic remediation in endangered species and conservation of genetic diversity were also proporsed.
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