| Project/Area Number |
12307001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Osaka University |
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Principal Investigator |
KANOSUE Kasuyuki Faculty of Medicine, Professor, 医学部, 教授 (50127213)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASHIMA Kei Faculty of Medicine, Research associate, 医学部, 助手 (40275194)
DOHI Yoshitane Faculty of Medicine, Professor, 医学部, 教授 (10028574)
INAGAKI Shinobu Faculty of Medicine, Professor, 医学部, 教授 (90151571)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥35,640,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2002: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2001: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2000: ¥17,700,000 (Direct Cost: ¥17,700,000)
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| Keywords | Thermoregulation / Efferent pathway / Effector / Hypothalamus / ラット / 遠心性信号 |
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| Research Abstract |
The preoptic area (PO) plays a key role in body temperature regulation by integrating information about local brain temperature and other body temperatures, and by sending efferent signals to various effector organs. Our previous studies elucidated that thermoregulatory skin vasodilation is elicited by the activation of warm-sensitive neurons in the PO, while shivering and non-shivering thermogenesis are controlled by the inhibitory actions of warm-sensitive neurons. In the midbrain skin vasodilative neurons were located in the the ventrolateral portion of the rostral periaqueductal grey (rPAG), and neurons having excitatory effect on non-shivering thermogenetive are located in the ventrolateral part of the caudal PAG (cPAG). However, the direct connections between the PO or the rest of the hypothalamus and the PAG in terms of thermoregulation has not been fully documented. In the present study we investigated in the rat's brain the distribution of neurons that are activated by exposure to neutral (26 ゜C), warm (33 ゜C) or cold (10 ゜C) ambient temperature, and that also project to the rPAG or cPAG, by the immunohistochemical analysis of Fos and a retrograde tracer, cholera toxin- b (CTb). When the tracer injection was centered in the rPAG, many double-labeled cells were observed in the dorsal part of the median preoptic nucleus (MnPO) in warm-exposed rats but few double-labeled cells were observed in the MnPO in cold-exposed rats. On the other hand, when the tracer injection was centered in the cPAG, many double-labeled cells were seen in the the posterior hypothalamic area (PH) in the cold-exposed rats but few double- labeled cells were seen in the warm-exposed rats. These results suggest that the rPAG receives warm signals from the MnPO and the cPAG receives cold signals from the PH region, probably participating in the efferent pathway for thermoregulatory skin vasomotion and non-shivering thermogenesis, respectively.
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