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Molecular basis for highly pathogenesis of avian influenza virus in humans.

Research Project

Project/Area Number 12307008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionThe University of Tokyo

Principal Investigator

KAWAOKA Yoshihiro  The University of Tokyo, IMS, Professor, 医科学研究所, 教授 (70135838)

Co-Investigator(Kenkyū-buntansha) KIDA Hiroshi  Hokkaido University, Professor, 大学院, 教授 (10109506)
HORIMOTO Taisuke  The University of Tokyo, IMS, Associate Professor, 医科学研究所, 助教授 (00222282)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥48,800,000 (Direct Cost: ¥42,800,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2002: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2001: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2000: ¥22,800,000 (Direct Cost: ¥22,800,000)
Keywordsavian influenza virus / H5N1 / reverse genetics / インフルエンザウイルス / リバース・ジェネティクス / 病原性 / 香港 / ヘマグルチニン / HA / 宿主域 / 組織指向性
Research Abstract

Influenza is an example of a disease for which the viral pathogen has emerged into the human population many times over past centuries, sometimes with devastating consequences
In 1997, an H5N1 influenza A virus was transmitted from birds to humans in Hong Kong, killing 6 out of the 18 people infected. When mice were infected with human isolates, two virulence groups became apparent. Using reverse genetics, we showed that a mutation at position 627 in the PB2 protein influenced the outcome of infection in mice. Moreover, high cleavability of the hemagglutinin glycoprotein was essential requirement for lethal infection.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (22 results)

All 2002 2001 2000 Other

All Journal Article (10 results) Publications (12 results)

  • [Journal Article] Human influenza A viral genes responsible for the restriction of its replication in duck intestine2002

    • Author(s)
      Hatta M, Halfmann P, Wells K, Kawaoka Y.
    • Journal Title

      Virology 295

      Pages: 250-255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses.2001

    • Author(s)
      Hatta M, Gao P, Halfmann P, Kawaoka Y.
    • Journal Title

      Science 293

      Pages: 1840-1842

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Amino acids responsible for the absolute sialidase activity of the influenza A virus neuraminidase : relationship to growth in duck intestine.2001

    • Author(s)
      Kobasa D, Wells K, Kawaoka Y
    • Journal Title

      Journal of Virology 75

      Pages: 11773-11780

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Plasminogen-binding activity of neuraminidase determines the pathogenicity of influenza A virus.2001

    • Author(s)
      Goto H, Wells K, Takada A, Kawaoka Y
    • Journal Title

      Journal of Virology 75

      Pages: 9297-9301

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Adaptation of influenza A viruses to cells expressing low levels of sialic acid leads to loss of neuraminidase activity2001

    • Author(s)
      Hughes MT, McGregor M, Suzuki T, Suzuki Y, Kawaoka Y
    • Journal Title

      Journal of Virology 75

      Pages: 3766-3770

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Amino acids responsible for the absolute sialidase activity of the influenza A virus neuraminidase : relationship to growth in duck intestine.2001

    • Author(s)
      Kobasa D, Wells K, Kawaoka Y.
    • Journal Title

      Journal of Virology 75

      Pages: 11773-11780

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Plasminogen-binding activity of neuraminidase determines the pathogenicity of influenza A virus.2001

    • Author(s)
      Goto H, Wells K, Takada A, Kawaoka Y.
    • Journal Title

      Journal of Virology 75

      Pages: 9297-9301

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Adaptation of influenza A viruses to cells expressing low levels of sialic acid leads to loss of neuraminidase activity2001

    • Author(s)
      Hughes MT, McGregor M., Suzuki T, Suzuki Y, Kawaoka Y
    • Journal Title

      Journal of Virology 75

      Pages: 3766-3770

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Sialic acid species as a determinant of the host range of influenza A viruses.2000

    • Author(s)
      Suzuki Y, Ito T, Suzuki T, Holland Jr, RE, Chambers TM, Kiso M, Ishida H, Kawaoka Y
    • Journal Title

      Journal of Virology 74

      Pages: 11825-11831

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Sialic acid species as a determinant of the host range of influenza A viruses2000

    • Author(s)
      Suzuki Y, Ito T, Suzuki T, Holland Jr, RE, Chambers TM, Kiso M, Ishida H, Kawaoka Y
    • Journal Title

      Journal of Virology 74

      Pages: 11825-11831

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hatta M, Kawaoka Y.: "The continued pandemic threat posed by avian influenza viruses in Hong Kong"Trends Microbiol.. 10(7). 340-344 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hatta M, Halfmann P, Wells K, Kawaoka Y.: "Human influenza a viral genes responsible for the restriction of its replication in duck intestine"Virology. 295(2). 250-255 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ito T, Kobayashi Y, Morita T, Horimoto T, Kawaoka Y.: "Virulent influenza A viruses induce apoptosis in chickens"Virus Res.. 84(1-2). 27-35 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Neumann G, Kawaoka Y.: "Synthesis of influenza virus : new impetus from an old enzyme, RNA polymerase I"Virus Res.. 82(1-2). 153-158 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Neumann G, Whitt MA, Kawaoka Y.: "A decade after the generation of a negative-sense RNA virus from cloned cDNA-what have we learned?"J Gen Virol.. 83(Pt11). 2635-2662 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hatta M, Neumann G, Kawaoka Y.: "Reverse genetics approach towards understanding pathogenesis of H5N1 Hong Kong influenza A virus infection"Philos Trans R Soc Lond B Biol Sci.. 356(1416). 1841-1843 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hatta M., Gao P., Halfmann P., Kawaoka Y.: "Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses"Science. 293. 1840-1842 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Schultz-Cherry S., Dybdahl-Sissoko N., Neumann G., Kawaoka Y., Hinshaw VS.: "Influenza Virus NS1 Protein Induces Apoptosis in Cultured Cells"Journal of Virology. 75. 7875-7881 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kobasa D., Wells K., Kawaoka Y.: "Amino Acids Responsible for the Absolute Sialidase Activity of the Influenza Virus Neuraminidase : Relationship to Growth in Duck Intestine"Journal of Virology. 75. 11773-11780 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Goto H., Wells K., Takada A., Kawaoka Y.: "Plasminogen-binding activity of neuraminidase determines the pathogenicity of influenza A virus"Journal of Virology. 75. 9287-9301 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ito T., Goto H., Yamamoto E., Tanaka H., Takeuchi M., Kuwayama M., Kawaoka Y., Otsuki K.: "Generation of a Highly Pathogenic Avian Influenza A Virus From an Avirulent Field Isolate by Passaging in Chickens"Journal of Virology. 75. 4439-4443 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hughes MT., McGregor M., Suzuki T., Suzuki Y., Kawaoka Y.: "Adaptation of influenza A viruses to cells expressing low levels of sialic acid leads to loss of neuraminidase activity"Journal of Virology. 75. 3766-3770 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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