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Identification and characterization of pluripotent hepatic stem cells in the liver

Research Project

Project/Area Number 12307023
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionUniversity of Tsukuba

Principal Investigator

TANIGUCHI Hideki  University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (70292555)

Co-Investigator(Kenkyū-buntansha) TAKADA Yasutsugu  University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (10272197)
NAKAUCHI Hiromitsu  University of Tsukuba, Institute of Basic Medical Sciences, Professor, 基礎医学系, 教授 (40175485)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥21,310,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2001: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2000: ¥11,300,000 (Direct Cost: ¥11,300,000)
KeywordsRegenerative Medicine / Liver / Stem cell / 再生
Research Abstract

Using flow cytometry combined with in vitro and in vivo single-cell-based assays, we prospectively identified pluripotent stem cells with multilineage differentiation potential and self-renewing capability in the developing mouse liver. These cells could be clonally propagated in culture, where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When cells that expanded in vitro were transplanted into recipient animals, they morphologically and functionally differentiated into hepatocytes and cholangiocytes, with reconstitution of hepatocyte and bile duct structures. Furthermore, these cells differentiated into pancreatic ductal and acinar cells or intestinal epithelial cells when transplanted into pancreas or duodenal wall. These data indicate that self-renewing pluripotent stem cells are retained in midgestational developing liver. Clonally isolated stem cells could be used to reveal the mechanism of cell differentiation in embryonic gut endoderm, and also could provide new insight into therapies for diseases of the digestive system.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] A.Suzukik H.Taniguchi, et al.: "Flowcytometric separation and enrichment of multipotent hepatic progenitor cells in mouse developing liver"Hepatology. 32. 1230-1239 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Taniguchi, et al.: "Usefulness of flowcytometric cell sorting for enrichment of hepatic stem cells in the liver"Transplantation Proceedings. 32. 249-251 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Taniguchi, et al.: "Clonogenic colony forming ability of flowcytometrically isolated hepatic stem/progenitor cells in the murine fetal liver"Cell Transplantation. 9. 897-700 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Suzuki, H.Taniguchi, et al.: "Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver"The Journal of Cell Biology. 156. 173-184 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Suzuki, H.Taniguchi, et al.: "Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting"Cell Transplant. 156. 173-184 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Suzuki, H.Taniguchi, et al.: "Hepatic stem/progenitor cells with high-proliferative potential in liver organ-formation"Transplan Proc. 33. 585-586 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, H.Taniguchi, et al: "Flowcytometric separation and enrichment of multipotent hepatic progenitor cells in mouse developing liver"Hepatology. 32(6). 1230-1239 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Taniguchi, et al: "Usefulness of flowcytometric cell sorting for enrichment of hepatic stem cells in the liver"Transplantation Proceedings. 32. 249-251 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Taniguchi, et al: "Clonogenic colony forming ability of flowcytometrically isolated hepatic stem/progenitor cells in the murine fetal liver"Cell Transplantation. 9. 697-700 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, H.Taniguchi, et al: "Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting"Cell Transplantation. 10. 393-396 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Suzuki, H.Taniguchi, et al: "Hepatic stem/progenitor cells with high-proliferative potential in liver organ-formation"Transplantation Proceedings. 33. 585-586 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Suzuki, H.Taniguchi, et al: "Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver"The Journal of Cell Biology. 156. 173-184 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Suzuki, H.Taniguchi, et al.: "Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver."The Journal of Cell Biology. 156. 173-184 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Suzuki, H.Taniguchi, et al.: "Clonal expansion of hepatic stem/progenitor cells following flow cytometric cell sorting"Cell Transplant. 10. 393-396 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Suzuki, H.Taniguchi, et al.: "Hepatic stem/progenitor cells with high-proliferative potential in liver organ-formation"Transplan Proc. 33. 585-586 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Suzuki,H.Taniguchi, et al: "Flowcytometric separation and enrichment of multipotent hepatic progenitor cells in mouse developing liver"Hepatology. 32. 1230-1239 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Taniguchi, et al: "Usefulness of flowcytometric cell sorting for enrichment of hepatic stem cells in the liver"Transplantation Proceedings. 32. 249-251 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Taniguchi, et al: "Clonogenic colony forming ability of flowcytometrically isolated hepatic stem/progenitor cells in the murine fetal liver"Cell Transplantation. 9. 697-700 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 谷口英樹,鈴木淳史: "多能性肝幹細胞の分化・増殖機構の解明"蛋白・核酸・酵素. 45. 256-262 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 谷口英樹,鈴木淳史: "「臓器置換」から「臓器再生」へ"細胞工学. 19. 869-873 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 谷口英樹: "肝臓における再生医療の展開"今日の移植. 13. 228-232 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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