| Project/Area Number |
12307024
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Mie University (2001-2002)
Kyoto University (2000) |
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Principal Investigator |
UEMOTO Shinji Mie University Faculty of Medicine Professor, 医学部, 教授 (40252449)
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| Co-Investigator(Kenkyū-buntansha) |
LI Xiao-kang 国立成育医療センター研究所, 移植・外科研究部, 室長 (60321890)
INOMATA Yukihiro Kumamoto University Faculty of Medicine Professor, 医学部, 教授 (50193628)
TANAKA Kouichi Kyoto University Faculty of Medicine Professor, 医学研究科, 教授 (20115877)
YOKOI Hajime Mie University Faculty of Medicine Assistant Professor, 医学部, 講師 (60174843)
KIMURA Hiromitsu Department of collaborative Research National research Institute for Child Health and development Director, 共同研究管理室, 室長 (80115477)
貝原 聡 京都大学, 医学研究科, 講師 (70324647)
鈴木 盛一 国立生育医療センター研究所, 移植・外科研究部, 部長 (00111386)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥33,720,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2002: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2001: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2000: ¥21,500,000 (Direct Cost: ¥21,500,000)
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| Keywords | gene / DANmicroarray / living donor liver transplantation / Immunosuppression / transplant tolerance |
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| Research Abstract |
Seventy recipients were enrolled in elective weaning immunosuppression at Kyoto University Hospital. Twenty-four recipients achieved complete withdrawal of immunosuppression, 37 were during weaning, and 9 encountered rejection. Fifty-four recipients were enrolled in nonelective weaning of immunosuppression. Thirty-four recipients achieved complete withdrawal, 7 were during weaning, and 13 encountered rejection. At Mie University Hospital, 3 recipients were enrolled in nonelective weaning because of EB virus infection. One recipient achieved complete withdrawal, but remaining 2 encountered rejection.
Total RNA was extracted from peripheral blood lymphocytes of recipients who accomplished complete withdrawal of immunosuppression and normal volunteers. RNA was analyzed by cDNA microarray (Agilent) comparing mRNA expression of recipients with that of normal volunteers. Number of genes with up-regulated mRNA expression was 120, and that with down-regulated expression was 169. Genes with up-regulated or down-regulated expression were divided into subgroups according to gene-related function. Number of up-regulated or down-regulated genes were 4/3 for [Apoptosis], 2/2 for [Cell cycle], 2/7 for [Cytokine], 4/4 for [DNA metabolism], 1/0 for [Heat shock protein], 3/7 for [Receptor], 16/18 for [Signal transduction], 7/14 for [Stress], 8/13 for [Transcription], 9/14 for [Transporter], and 72/113 for undetermined factor, respectively.
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