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Novel approach to control ischemia-reperfusion injury in small intestinal transplantation

Research Project

Project/Area Number 12307025
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKeio University

Principal Investigator

KITAJIMA Masaki  Department of Surgery, Keio University School of Medicine, Professor, 医学部, 教授 (90112672)

Co-Investigator(Kenkyū-buntansha) WAKABAYASHI Go  Department of Surgery, Keio University School of Medicine, Assistant Professor, 医学部, 講師 (50175064)
SHIMAZU Motohide  Department of Surgery, Keio University School of Medicine, Assistant Professor, 医学部, 講師 (70124948)
KOYASU Shigeo  Department of Immunology, Keio University School of Medicine, Professor, 医学部, 教授 (90153684)
TANABE Minoru  Department of Surgery, Keio University School of Medicine, Teaching Staff, 医学部, 助手 (50197513)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥37,890,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2001: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2000: ¥23,200,000 (Direct Cost: ¥23,200,000)
Keywordssmall bowel transplantation / ischemia-reperfusion injury / mitogen activated protein kinase (MAPK) / interleukin-1 (IL-1) / tumor necrosis factor-α (INF-α) / apoptosis / 虚血再灌流障害 / interleukin-1(IL-1) / 免疫抑制療法 / MAP kinase
Research Abstract

Because small intestine is one of the most susceptible organs to ischemia, ischemia-reperfusion injury is formidably limiting factor in small intestinal transplantation. By using rat small intestinal ischemia-reperfusion injury model, we demonstrated that TNF-α and IL-1β is produced in the rat small intesyinal ischemia-reperfusion injury and that the production can be reduced by the specific TNF-α and IL-1β inhibitor, attenuating the intestinal injury. Recently, mitogen-activated protein kinase (MARK) superfamily has been widely noticed as upupstream signal transduction mechanisms for TNF-α and IL-1β have been reported. Especially, c-Jun N-terminal kinase (JNK) and p38 induce cell apoptosis by various stimuli including ischemia. In addition, p38 acts as a key enzyme to produce IL-1 and TNF, suggesting these enzymes play a key role in ischemia-reperfusion Injury. In the present study, we investigated the pathophysiologic significance of JNK and p38 in rat small intestinal ischemia-reperfusion injury. Remarkable activation of JNK and p38 was shown in the injured small intestinal tissue after ischemia-reperfusion by using kinase assay. Immunohistochemistry of the intestinal tissue after reperfusion revealed that the activated p38 was mainly located in the epithelial cells of the villus tip, where TUNNEL positive apoptotic cells were present. Administration of LL-Z1640-2, a dual inhibitor of JNK and p38, effectively suppressed the activation of these enzymes, and reduced the number of apoptotic cells on villus tip, resulting that the mucosal morphology was well preserved. These results indicate that JNK and p38 play a key role in small intestinal ischemia-reperfusion injury, and that the simultaneous inhibition of these enzymes may provide a novel therapeutic approach to overcome ischemic damage in small intestinal transplantation.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 亀井秀策: "小腸虚血再潅流障害における組織内MAPKの活性変動と組織障害の観察"Cyto-protection & biology. 18. 73-75 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 田辺 稔: "小腸移植の現況"G.I.Research. 9(1). 53-60 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 篠田昌宏: "各種病態に対する生体肝移植周術期におけるサイトカイン変動の検討"移植. 36(3). 159-165 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 島津元秀: "肝虚血・再潅流障害におけるnitric oxide(NO)の役割"G.I.Research. 9(4). 363 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto S: "The role of tumor necrosis factor-α and interleukin-1β in ischemia-reperfusion injury of the rat small intestine"Journal of Surgical Research. 99. 134-141 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kamei S., et al.: "Pathophysiological role of MARK in small intestinal ischemia-refierfusion injury"Cyto-protection & Biology. 18. 73-75 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shinoda M., et al.: "Cytokine release in perioperative phase of living-related liver transplantation"Ishoku. 36 (3). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimazu M., et al.: "The role of nitric oxide in hepatic ischemiareperfusion injury"G.I. Research. 9 (4). 363 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto S., et al.: "The role of tumor necrosis factor-α and interleukin-1β in iscnemia-reperfusion injury of the rat small intestine"Jouranal of Surgical Research. 99. 134-141 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanabe M., et al.: "Current status of small bowel transplantation"G.I. Research. 9 (1). 53-61 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 田辺 稔: "小腸移植の現況"G.I.Research. 9(1). 53-60 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 篠田昌宏: "各種病態に対する生体肝移植周術期におけるサイトカイン変動の検討"移植. 36(3). 159-165 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 島津元秀: "肝虚血・再灌流障害におけるnitric oxide(NO)の役割"G.I.Research. 9(4). 363 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamamoto S: "The role of tumor necrosis factor-α and interleukin-1 β in ischemia-reperfusion injury of the rat small intestine"Journal of Surgical Research. 99. 134-141 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamamoto S: "The role of tumor necrosis factor-a and interleukin-1b in ischemia-relerfusion injury of the rat small intestine"J Surg Res. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 島津元秀: "【肝移植と集中治療】肝移植後の血管合併症の診断と対策"集中治療. 11(7). 668-673 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 島津元秀: "生体部分肝移植の手技-ドナーの手技"外科治療. 82(2). 139-143 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田辺稔: "【臓器移植】小腸移植の現況"GI Research. 9(1). 53-60 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shinoda M: "Ischemic preconditioning against hepatic microcirculation disturbance after warm ischemia/reperfusion in rat"Microcirculation annual. 16. 119-120 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 篠田昌宏: "生体肝移植における術中・術後の凝固線溶系の変動とその管理"移植. 35(5). 243-254 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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