| Project/Area Number |
12307030
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
OKAWA Akihiko Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (30312945)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZARIS Masashi Chiba University, University Hospital, Assistan, 医学部付属病院, 助手 (50281712)
MORIYA Hideshige Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (30092109)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥37,260,000 (Direct Cost: ¥35,700,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2002: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2001: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2000: ¥30,500,000 (Direct Cost: ¥30,500,000)
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| Keywords | osteoarthritis / ANKH gene / association study / SNPs (single nucleotide polymorphisms) / genomic DNA / 疾患遺伝子 / モデルマウス / Ank / 一塩基多型 / ankマウス / 罹患同胞対連鎖解析 / 遺伝子 / ank マウス |
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| Research Abstract |
In order to search die genes responsible for osteoarthritis (OA), association study between the gene responsible for the model mouse and osteoarthritis were performed and sib-pair linkage analysis were projected,
1. Collecting genomic DNA of osteoarthritis patients
Genomic DNAs of 173 sporadic cases and 29 family cases (38 sibpairs), who have osteoarthritis without influence of injury, were collected,
2. Analysis of the gene responsible for the osteoarthritis model mouse
Association, study between the gene responsible for the model mouse and osteoarthritis was performed, A human homologue of mouse ank, which is responsible, for the OA model mouse, was cloned. The nucleotide sequence of full-length cDNA and promoter region of the ANKH gene was elucidated. Using 40 samples of genomic DNA, sequence variances in the ANKH gene were searched by PCR-SSCP and direct sequencing method. Five polymorphisms were found, A1303G in coding region, G1831A in 31untranslated region, eight-base-pairs deletionin 5' untranslated region, -785A/G and -639 delC in the promoter region. Using 202 samples of 0 A patients and 103 samples of non-OA controls, association between these polymorphisms and OA were analyzed by qui-square test. An association between eight-base-pairs deletion in 51untranslated region and OA was So it found. So it was found. Suggested that the ANKH gene has influence in the etiology of osteoarthritis. Found.
3. Sib-pair linkage analysis
Collected samples of family cases are not enough to perform the sib -pair linkage analysis. Further collection is necessary.
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