| Project/Area Number |
12308001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
体育学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ATOMI Yoriko The University of Tokyo, Graduate School of Arts and Sciences, Professor, 大学院・総合文化研究科, 教授 (90125972)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Hideaki The University of Tokyo, Graduate School of Arts and Sciences, Assistant Researcher, 大学院・総合文化研究科, 助手 (60313160)
HATTA Hideo The University of Tokyo, Graduate School of Arts and Sciences, Assistant Professor, 大学院・総合文化研究科, 助教授 (60208535)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥44,530,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2001: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2000: ¥18,400,000 (Direct Cost: ¥18,400,000)
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| Keywords | stress protein / running exercise / C57B6 inbred mice / skeletal muscle / HSP72 / hindlimb suspension model / tubulin / lacate threshold(LT) / LT(Lactate Threshold) / 副腎 / ACTH / HPA軸 / C57B6マウス / 機械刺激 / 運動負荷 / 酸素摂取量 / 実験動物 / エネルギー代謝 / 無酸素性作業閾値 |
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| Research Abstract |
In the present study new methods to study the effect of stress protein on adaptability and were developed and three different results were obtained. 1) In the hindlimb suspension model, cytoskeletal protein, tubulin also decreased as well as αB-crystallin. αB-crystallin has chaperone function for tubulin as previously reported (Arai & Atomi, 1997). Muscle shortening suppresses the expression of αB-crystallin. This might facilitate tubulin denaturing as substrate of αB-crystallin. 2) The suppression of αB-crystallin by way of antisense method in cultured cells, induced the change of cell shape and weakened cell adhesion, resulting in inducing abnormal motility using time-lapse analysis. The cell that over expresses αB-crystallin widely develops, strongly adheres. It is shown that αB-crystallin is essential for tonic cell contraction and adhesion. 3) New method to study the responses of mice (C57B6) to exercise was developed for the inbred mice that genetically identified. Anaerobic Threshold (AT) was measured by the use of VO_2 VCO_2 and identified to be me same as LT by the analysis of blood lactate concentration. Interestingly Hsp72 expressions was significantly increased after 30' treadmill running exercise only at lower intensity than LT level, but not higher than LT. This phenomenon is similar to stress tolerance, which is obtained after mild stress previously reported.
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