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GENETIC CASCADE UNDERLYING NOTOCHORD FORMATION IN ASCIDIAN EMBRYOS

Research Project

Project/Area Number 12308038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SATOH Noriyuki  KYOTO UNIVERSITY, GRADUATE SCHOOL OF SCIENCE, PROFESSOR, 大学院・理学研究科, 教授 (30025481)

Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥45,170,000 (Direct Cost: ¥39,500,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2002: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2001: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2000: ¥20,600,000 (Direct Cost: ¥20,600,000)
KeywordsAscidians / Notochord formation / Genetic cascade / Brachyury / beta-catenin / FoxD / ZicL / Convergent extension / 脊索動物の進化 / 下流標的遺伝子 / Zic / 誘導 / β-カテニン / 遺伝子発現 / 機能制御 / 脊索 / 特異的遺伝子 / 発現制御 / 脊索動物 / 進化
Research Abstract

Notochord is the most prominent feature of chordates. The present study aimed to explore the genetic cascade underlying notochord formation in ascidian embryos. Previous studies, mostly from my own laboratory, demonstrated that Brachyury gene of Ciona intestinalis (Ci-Bra) begins to be expressed at the 64-cell stage and plays-an essential role in the notochord differentiation. In the present study, we investigated (1) Ci-Bra upstream cascade which leads to the initiation of Ci-Bra expression and (2) Ci-Bra downstream cascade which leads to morphological formation of the notochord
(1) Ci-Bra upstream cascade : In the present study, we make clear that (1) maternally supplied beta-catenin is translocated after fertilization from the cytoplasm into nuclei of vegetal blastomeres and specifies the endoderm developmental fate, (2) beta-catenin triggers the activation of various target genes including FoxD, ZicL, and Fgf9/16/20, (3) FoxD is a direct target of beta-catenin and is expressed in th … More e endoderm cells at the 16-and 32-cell stage, and the gene function is essential for Ci-Bra activation, (4) ZicL is downstream of FoxD and is expressed in notochord/nerve cord cells at the 32-cell stage, and this gene function is essential for Ci-Bra activation. Therefore, the present study has revealed that Ci-Bra upstream genetic cascade consists of beta-catenin, FoxD, ZicL and Ci-Bra in this order
(2) Ci-Bra downstream cascade : We have succeeded in the isolation and characterization of Ci-Bra downstream genes including prickle, ERM, tyrosine phosphate, and cdc45. The 5' upstream sequences of these genes contain Ci-Bra protein binding sequences, suggesting that they are direct targets of Ci-Bra. When function of these Ci-Bra downstream genes is suppressed, convergent-extension movement of the notochord cells is prohibited. Therefore, most of the Ci-Bra downstream genes act as members involved in morphogenetic movements
The present study is therefore an achievement of genetic cascade underlying the notochord formation in the ascidian embryo Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Shimauchi, Y.: "FGF signals are involved in the differentiation of notochord cells and mesenchyme cells of the ascidian Halocynthia roretzi"Development. 128. 2711-2721 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishino, A.: "Brachyury (T) gene expression and notochord development in Oikopleura longicauda (Appendicularia, Urochordata)"Development Genes and Evolution. 211. 219-231 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishino, A.: "The simple tail of chordates : Phylogenetic significance of appendicularians"genesis. 29. 36-45 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh, N.: "Ascidian embryos as a model system to analyze expression and function of developmental genes"Differentiation. 68. 1-12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Dehal, P.: "The draft genome of Ciona intestinalis : insights into chordate and vertebrate origins"Science. 298. 2157-2167 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai, K.S.: "Early embryonic expression of FGF4/6/9 gene and its role in the induction of mesenchyme and notochord in Ciona savignyi embryos"Development. 129. 1729-1738 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai, K.S.: "Multiple functions of a Zic-like gene in the differentiation of notochord, central nervous system and muscle in Ciona savignyi embryos"Development. 129. 2723-2732 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai, K.S.: "An essential role of a FoxD gene in notochord induction in Ciona embryos"Development. 129. 3441-3453 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takada, N.: "Brachyury expression in tailless molgulid ascidian embryos"Evolution & Development. 4. 205-211 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh, N.: "The ascidian tadpole larva : Comparative molecular development and genomics"Nature Reviews Genetics. 4. 285-295 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh, N.: "Ciona intestinalis : an emerging model for whole-genome analyses"Trends in Genetics. 19. 376-381 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimauchi, Y., Murakami, S.D., Satoh, N.: "FGF signals are involved in the differentiation of notochord cells and mesenchyme cells of the ascidian Halocynthia roretzi"Development. 128. 2711-2721 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishino, A., Satou, Y., Morisawa, M., Satoh, N.: "Brachyury (T) gene expression and notochord development in Oikopleura longicauda (Appendicularia, Urochordata)"Development Genes and Evolution. 211. 219-231 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishino, A., Satoh, N.: "The simple tail of chordates : Phylogenetic significance of appendicularians"genesis. 29. 36-45 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh, N.: "Ascidian embryos as a model system to analyze expression and function of developmental genes"Differentiation. 68. 1-12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Dehal, P., Satou, Y., Campbell, R.K., Kohara, Y., Levine, M., Satoh, N., Rokhsar, D.S.: "The draft genome, of Ciona intestinalis : insights into chordate and vertebrate origins"Science. 298. 2157-2167 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai, K.S., Satoh, N., Satou, Y.: "Early embryonic expression of FGF4/6/9 gene and its role in the induction of mesenchyme and notochord in Ciona savignyi embryos"Development. 129. 1729-1738 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai, K.S., Satou, Y., Satoh, N.: "Multiple functions of a Zic-like gene in the differentiation of notochord, central nervous system and muscle in Ciona savignyi embryos"Development. 129. 2723-2732 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai, K.S., Satoh, N., Satou, Y.: "An essential role of a FoxD gene in notochord induction in Ciona embryos"Development. 129. 3441-3453 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takada, N., York, J., Davis, J.M., Schumpert, B., Yasuo, H., Satoh, N., Swalla, B.J.: "Brachyury expression in tailless molgulid ascidian embryos"Evolution & Development. 4. 205-211 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh, N.: "The ascidian tadpole larva : Comparative molecular development and genomics"Nature Reviews Genetics. 4. 285-295 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh, N., Satou, Y., Davidson, B., Levine, M.: "Ciona intestinalis : an emerging model for whole-genome analyses"Trends in Genetics. 19. 376-381 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai, K.S.: "Early embryonic expression of FGF4/6/9 gene and its role in the induction of mesenchyme and notochord in Ciona savignyi embryos"Development. 129. 1729-1738 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Imai, K.S.: "Multiple functions of a Zic-like gene in the differentiation of notochord, central nervous System and muscle in Ciona savignyi embryos"Development. 129. 2723-2732 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Imai, K.S.: "An essential role of a FoxD gene in notochord induction in Ciona embryos"Development. 129. 3441-3453 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Noriyuki Satoh: "Ascidian embryos as a model system to analyze expression and function of developmental genes"Differentiation. 68. 1-12 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yutaka Satou: "Early expression of a LIM-homeobox gene Cs-1hx3 is downstream of b-catenin adn responsible for endoderm differentiation"Development. 128. 3559-3570 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshie Shimauchi: "FGF signals are involved in the differentiation of notochord cells and mesenchyme cells"Development. 128. 2711-2711 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshie Shimauchi: "Synergistic action of HNF-3 and Brachyury in the notochord differentiation of ascidian embryos"Intern.Journ.Develop.Biol.. 45. 643-652 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hotta,Kohji: "Characterization of Brachyury-downstream notochord genes in the Ciona intestinalis embryo"Developmental Biology. 234. 69-80 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishino,Atsuo: "The simple tail of chordates : Phylogenetic significance of appendicularians"Genesis. 29. 36-45 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishino,Atsuo: "Brachyury (T) gene expression and notochord development in Oikopleura longicauda"Development, Genes and Evolution. 211(in press). (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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