| Project/Area Number |
12309004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
ABE Masanori Tokyo Institute of Technology, Graduate School of Science and Engineering, Prof,, 大学院・理工学研究科, 教授 (70016624)
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| Co-Investigator(Kenkyū-buntansha) |
HIBI Toshihumi Keio University, School of Medicine, Prof., 医学部, 教授 (50129623)
HANDA Hiroshi Frontier Collaborative Research Center, Prof., フロンティア創造共同研究センター, 教授 (80107432)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥32,100,000 (Direct Cost: ¥27,900,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2002: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2001: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2000: ¥13,900,000 (Direct Cost: ¥13,900,000)
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| Keywords | medical magnetic beads / ferrite beads / IGG antibody / anticancer drug / hyperthermia / magnetic bioprobe / antibody / antigen / 蛍光磁性プローブ / マグネタイト / バイオ・リガンド / バイオ高分子 / スピネル型 / BSA / CMC / 生体高分子 / 磁気分離 |
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| Research Abstract |
1. Development of novel medical ferrite Nano-Beads
We established the technique to immobilize physiologically active molecules onto the surfaces of ferrite (Fe_3O_<4+8>) nano beads (10-15 run in diameter) during the beads are synthesized from an aqueous solution. We prepared medical magnetic nano beads on the surfaces of which we strongly immobilized such physiologically active molecules as Trypsin FITC-avidin, anti-cancer drugs, glycidyl methacrylate (GMA), amino acids (e.g. aspartic acid) and similar-strusture molecules, polyacryl amino acid, etc.
2. We revealed that in the above-mentioned fenite nano beads the physiologically active molecules are strongly couples to ferrite intermediated by S-H and COOH groups of the amino acids or similar-structure molecules contained in the physiologically active molecules. We named the intermediating amino acids (and similar structured molecules) connectors. Also we called the medical nano beads (with physiologically active molecules coupled to the
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surfaces intermediated by the connectors) "Connectors-Bonded Magnetic (CBM)" nano beads. Furthermore, we developed CBM nano beads having surfaces coated with (1)connectors and (2)polymers exhibiting low sensitivity to non-specific absorption of physiologically active molecules ; on their surfaces we can easily immobilize physiologically active molecules by biochemical technique.
3. Preparation of human-type antibody and novel ferrite bioprobes
We have prepared human-type IGG antibody to human colon cancer cells by replacing the constant region of mouse monoclonal IGG antibody with that of human IGG antibody. The human-type IGG antibody fixed on the ferrite beads selectively reacted with the antigens present on human colon cancer cell membrane, which were visualized by staining with anti-IGG antibody conjugated with fluorescence. This shows that die IGG antibody-fixed ferrite beads are utilized for detection of cancer as diagnostic magnetic bioprobes.
4. Power absorption rate of ferrite nano beads for hyperthermia
We found that the fenite nano beads prepared in this study have specific power absorption rate for AC magnetic field induction 1.4 times higher than that of conventional ferrite beads. This indicates that our ferrite nano beads are advantageous for applying to hyperthermia. Less
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