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Improvement of photosynthesis by modification of light harvesting systems

Research Project

Project/Area Number 12354009
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 植物生理
Research InstitutionHokkaido University

Principal Investigator

TANAKA Ayumi  Hokkaido Univ., Inst., of Low Temp. Sci., Prof., 低温科学研究所, 教授 (10197402)

Co-Investigator(Kenkyū-buntansha) HOSAKA Hideo  Nihonsoda Co., Ltd., Odawara Inst., General manager, 小田原研究所, 部長
TANAKA Ryouichi  Hokkaido Univ., Inst., of Low Temp. Sci., Prof., 低温科学研究所, 助手 (20311516)
HARA Toshihiko  Hokkaido Univ., Inst., of Low Temp. Sci., Prof., 低温科学研究所, 教授 (80183094)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥37,800,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2002: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2001: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2000: ¥22,200,000 (Direct Cost: ¥22,200,000)
KeywordsCAO / photosynthesis / chlorophyll / light energy / light-harvesting apparatus
Research Abstract

Photosynthetic organisms can proceed photosynthesis under various light conditions on the Earth. Plants employ two strategies to adapt light intensities, one is the dissipation of excess light energy as heat and the other mechanism is the regulation of light harvesting apparatus. Capturing light energy is the first step of photosynthesis and the regulation of the amount of harvesting light energy directly influences the rate of photosynthesis. There are many reports on the employment of genetic engineering for the improvement of agricultural production . Most typical successes were to improve the resistances to virus infection and insect attack. However, these genetic engineerings do not increase productivity itself. In order to increase the productivity of the plants, the ability of photosynthesis must be modified. The aims of this project are 1) to clarify the mechanisms of the acclimation of antenna size to light intensities, 2) modification of antenna size to improve productivities. Green plants have large antenna size under low light conditions in order to capture light energy for photosynthesis. In this case, the level of CAO mRNA was high and chlorophyll b was actively synthesized. However, in CAO over expressed lines of Arabidopsis thaliana, the level of CAO mRNA and the antenna size was almost same under wide range of light intensities. We concluded from the above experiments that CAO plays a central role in regulating the antenna size under various light intensities. We also found CAO over-expressed mutants grow well under low light intensities compared to wild type. It indicates that modifications of photosynthesis are potentially powerful tool to increase the productivities of the plants.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] U.Oster: "How chlorophyll b is made : Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana"Plant J.. 21. 305-310 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] E.W.Juergen: "Photosynthetic apparatus organization and function in wild type and a Chlorophyll b-less mutant of Chlamydomonas reinhardtii. Dependence on carbon source"Planta. 211. 335-344 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] R.Tanaka: "Overexpression of chlorophyllide a oxygenase (CAO) enlarges the antenna size of photosystem II in Arabidopsis thaliana"Plant J.. 26. 365-374 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Satoh: "Chlorophyll b expressed in cyanobacteria functions as a light-harvesting antenna in photosystem I through flexibility of the proteins"J.Biol.Chem.. 276. 4293-4297 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Satoh: "Chlorophyll b inhibits the formation of photosystem I trimer in Synechocystis sp.PCC6803"FEBS Letters. 528. 235-240 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Masuda: "Irradiance-dependent adjustment of chlorophyll antenna size in Dunaliella salina is regulated by coordinate expressions of chlorophyll a oxygenase (CAO) and Lhcb genes with singnaling pathways"Plant Mol.Biol.. 51. 757-771 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Oster, U., Tanaka, R., Tanaka, A., Rudiger, W.: "How chlorophyll b is made : Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana"Plant. J.21. 305-310 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Juergen, E. W. Polle, W., Benemann, J. R., Tanaka, A., Melis A.: "Photosynthetic apparatus organization and function in the wild type and a chlorophyll b-less mutant of Chlamydomonas reinhardtii. Dependence on caron source"Planta. 211. 335-344 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka R., Koshino Y., Sawa S., Ishiguro S., Okada K., Tanaka A.: "Overexpression of chlorophyllide a oxygenase (CAO) enlarges the antenna size of photosystem II in Arabidopsis thaliana"Plant. J.26. 365-374 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh S., Ikeuchi M., Mimuro M., Tanaka A.: "Chlorophyll b expressed in cyanobacteria functions as a light-harvesting antenna in photosystem I through flexibility of the proteins"J. Biol. Chem.. 276. 4293-4297 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satoh, S., Tanaka, A.: "Chlorophyll b inhibits the formation of photosystem I trimer in Synechocystis sp. PCC6803 FEBS Letters"FEBS Leters. 528 in press. 235-240 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masuda, T., Tanaka, A., Melis, A.: "Irradiance-dependent adjustment of chlorophyll antenna size of Dunaliella salina is regulated by coordinate expression of chlorophyll a oxygenase (CAO) and Lhcb genes with shared signaling pathways"Plant Mol. Biol. 51. 757-771 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Satoh: "Chlorophyll b inhibits the formation of photosystem I trimer in Synechocystis sp.PCC6803"FEBS Lett.. 528. 235-240 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Masuda: "Irradiance-dependent adjustment of chlorophyll antenna size of Dunaliella salina is regulated by coordinate expression of chlorophyll a oxygenase(CAO)and Lhcb genes with shared signaling pathways"Plant Mol. Biol. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] 田中 歩: "再現実験から光合成の進化を考える"遺伝. 56. 67-72 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] R.Tanaka: "Over expression of chlorophyllide a oxygence (CAO) enlarges antenna size of photo system II in Arabidopsis Thaliana"Plant J. 26. 365-374 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田中 歩: "新しい光合成色素の獲得と植物の進化"生命誌. 30. 8-9 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] R.Tanaka: "Overexpression of chiorophyllidea oxygenase (CAO) enlarges the antenna size of photosystem II in Arabidopsis thaliana"The plant J. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.Satoh: "Chlorophyll b expressed in cyanobacteria functions as eight-harvesting antenna in photosystemII through flexibility of the proteins."J.Biol.chem.. 276. 4293-4297 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] U.Oster: "How chlorophyll b is made : cloning and functinal expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis theliana."The plant J.. 21. 305-310 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] E.W.Juergen: "Photosynthetic apparatus organization and function in the wild type and a chlorophyll b less mutant of chlamydomonas reinhardtii."Planta. 211. 335-344 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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