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Development and Practical Use of Highly Sensitive Detection Methods for Individualization Markers

Research Project

Project/Area Number 12357003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Legal medicine
Research InstitutionFaculty of Medical Sciences, University of Fukui

Principal Investigator

YASUDA Toshihiro  University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (80175645)

Co-Investigator(Kenkyū-buntansha) UEKI Misuzu  University of Fukui, Faculty of Medical Sciences, Instruction Official, 医学部, 教務職員 (00165656)
JIDA Reiko  University of Fukui, Faculty of Medical Sciences, Assistant, 医学部, 助手 (40139788)
KISHI Koichiro  Gunma University Graduate School of Medicine, Professor, 医学部, 教授 (30169841)
SAWAZAKI Kazumi  Fukui Prefectural Police Headquarters, Scientific Investigation Laboratory, Section Head, 科学捜査研究所, 主任研究官
澤崎 カズミ  福井県警察本部科学捜査研究所, 主任研究員
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥35,500,000 (Direct Cost: ¥28,900,000、Indirect Cost: ¥6,600,000)
Fiscal Year 2003: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2002: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2001: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2000: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsPersona identification / Forensic application / Deoxyribonuclease / Urine / Genetic polymorphism / Molecular biology / Human genetics / Genetic markers / 核酸分解酵素 / DNaseI / DNA多型 / 多型 / 分子進化 / DNase I / DNase II / モノクローナル抗体 / 脳下垂体 / deoxyribonuclease I / 抗体 / 哺乳類細胞発現系 / ソマトスタチン / 鑑識科学 / Deoxyribonuclease I / Deoxyribonuclease II / 多型形質 / 個人差 / 遺伝子発現
Research Abstract

1.We have previously found human deoxyribonuclease(DNase) II to be a genetic marker : DNase II levels in human vary depending on whether the individual has the DNA SF2 *H or *L allele.It was clarified that the A-75G transition in the proximal promoter region causes the allelic difference in the promoter activity of the gene, underlying its genetic polymorphism.
2.We devised a simple DNA extraction procedure suitable for STR typing of urine samples using a commercially available DNA/RNA extraction kit. This method alows urine samples to be kept in both a frozen or aqueous state for forensic use.Furthermore, we devised a procedure that combines a simple extraction method, isoelectric focusing and activity-staining for DNase I typing from aged urine stains.DNase I polymorphism could be considered the first biochemical marker found to be well suited for individualization from small aged urine stains.
3.We confirmed the population genetic basis for DNase I polymorphism in a Japanese populatio … More n for forensic application. Our examination of DNase I types revealed a decreasing north-to-south gradient in the DNA SE1 allele.
4.We discovered a novel variable number of tandem repeat of 56 bp unit in intron 4 of human DNase I gene, resulting in elevation of individualization efficiency of DNase.
5.In order to devise the highly sensitive-and specific-immunological typing for DNase I and II polymorphisms, we succeeded in production of each murine monoclonal anti-human DNase I and DNase II with high specificity using newly developed screening procedure for antibodies.Immunoaffinity procedure using these monoclonal antibodies made the purification of human DNases I and II easier, faster and more effective than the conventional procedure. Furthermore, in order to obtain human DNase I as an immunogen, we developed a procedure consisting of transient expression of the enzyme in transfected mammalian cells with DNase I cDNA and purification of the enzyme from the culture medium.
6.Molecular-evolutional aspects of vertebrate DNase I family have been clarified. Less

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (149 results)

All Other

All Publications (149 results)

  • [Publications] R.Iida: "Age-related changes of gene expression in mouse kidney : fluorescence differential display-PCR analysis"Mech.Aging Development. 113・2. 135-144 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Lett.. 467・1/2. 231-234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita: "Mammalian deoxyribonuclease I are classified into three types"Biochem.Biophys.Res.Commun.. 269・2. 481-484 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakajima: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Exp.Clin.Immunogenet. 17・2. 71-76 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita: "Japanese population data for two STR loci, Hum TPO and HumLPL"Legal Med.. 2・2. 64-67 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Tsutsumi: "Association of the DNase I phenotype 2 with colorectal carcinoma risk in Japanese populations"Cancer Lett.. 159・1. 109-112 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I"DNA Sequences. 11・3/4. 247-255 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita: "Postmortem absorption of dichloromethane"Int.J.Legal Med.. 114・1/2. 96-100 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 竹下治男: "DNASE1^*6遺伝子がつくる酵素の寿命は短い"DNA多型. 8. 207-210 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 飯田礼子: "年齢依存性発現を示すマウス腎の新規遺伝子検索"DNA多型. 8. 123-126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "Tissue-specific in vivo inhibition of DNase I gene expression by somatostatin"Biochem.Biophys.Res.Commun.. 283・2. 287-291 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Mori: "Molecular, biochemical and immunological analyses of porcine pancreatic DNase I"Biochim.Biophys.Acta. 1547・2. 275-287 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita: "Geographical north-south decline in DNase I allele 2 in Japanese population"Hum.Biol.. 73・1. 129-134 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida: "Cloning, mapping, genomic organization and expression of mouse M-LP, a new member of the peroxisomal membrane protein Mpv17 domain family"Biochem.Biophys.Res.Commun.. 283・2. 292-296 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "Amphibian DNase I are characterized by a C-terminal end with a unique, cystein-rich stretch and by the insertion of a serine residue into the Ca^<2+>-binding site"Biochem.J.. 357・2. 473-480 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita: "Use of human recombinant DNase I expressed in COS-7 cells as an immunogen to produce a specific anti-DNase I antibody"Exp.Clin.Immunogenet.. 18・4. 226-232 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida: "Identification and characterization of two novel human polymorphic STRs on Y chromosome"Int.J.Legal Med.. 15・1. 54-56 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "Activity staining for detection of ribonucleases using the dried agarose film overlay(DAFO) method after isoelectric focusing"Methods in Enzymology. 341. 94-112 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Tsutsumi: "DNase I is present in the chief cells of human and rat stomach"Histochem.J.. 33・9/10. 531-535 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kishi: "DNase I : structure, function, and use in medicine and forensic science"Legal Med.. 3・2. 69-83 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 飯田礼子: "マウス腎の年齢依存性発現遺伝子"DNA多型. 9. 59-63 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 竹下治男: "STR座位HumTPOおよびHumLPL:日本人7集団のデータについて"DNA多型. 9. 178-181 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 岸紘一郎: "尿DNase I"生物物理化学. 45・1. 55-59 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita: "A death resulting from inadvertent intravenous infusion of external feed"Int.J.Legal Med.. 116・1. 36-38 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "Abrupt pubertal elevation of DNase I gene expression in human pituitary glands of both sexes"FEBS Lett.. 510・1. 22-26 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakajima: "Production and characterization of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine"Biochim.Biophys.Acta. 1570・3. 160-164 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida: "Characterization and haplotype analysis of the polymorphic Y-STRs DYS443, DYS444 and DYS445 in a Japanese population"Int.J.Legal Med.. 116・3. 191-194 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida: "Five age-dependently expressed genes in mouse brain revealed by the fluorescence differential display-PCR technique"Exp.Gerontol.. 37・8/9. 1121-1126 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 茂木康一: "脊椎動物DNase Iの分子進化:両生類DNase IはユニークなC末端延長領域をもつ"DNA多型. 10. 88-92 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 飯田礼子: "Y染色体上の新規多型性STR5種類の同定とハプロタイプ解析"DNA多型. 10. 160-163 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "A simple method of DNA extraction and STR typing from urine samples using a commercially available DNA/RNA extraction kit"J.Forensic Sci.. 48・1. 108-110 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "A single amino acid substitution of Leu130Ile in snake DNase I contributes to the acquisition of thermal stability"Eur.J.Biochem.. 270・2. 307-314 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida: "M-LP Mpv17-like protein, has a peroxisomal membrane targeting signal comprising a transmembrane domain and a positively charged loop and up-regulates expression of the manganese superoxide dismutase gene"J.Biol.Chem.. 278・8. 6301-6306 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakajima: "Production of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine"Legal Med.. 5・Suppl.1. s214-s216 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Mogi: "Carp hepatopancreatic DNase I"J.Biochem.. 133・3. 377-386 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "Usefulness of deoxyribonuclease I (DNase I) polymorphism for individualization from small aged urine stains"Legal Med.. 5・2. 105-107 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida: "A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444 and DYS445"J.Forensic Sci.. 48・5. 1088-1090 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Yoshida: "An abrupt elevation of the serum deoxyribonuclease I (DNase I) activity in acute myocardial infarction"Circulation J.. 67・Suppl.1. 300 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Mogi: "Case report : food poisoning to death by Podostroma cornu-damae, its case history and autopsy findings"Acta Crim.Japon. 69・1. 14-20 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Kaneko: "Molecular, biochemical and immunological analyses of canine pancreatic DNase I"J.Biochem.. 134・5. 711-718 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 中島たみ子: "ヒトDNase IIの多型はプロモーター領域のSNP(A-75G)によって発生した"DNA多型. 11. 230-234 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 飯田礼子: "マウス腎年齢依存性発現分子M-LPの細胞内局在と生理的機能"DNA多型. 11. 113-117 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 岸紘一郎: "DNase Iの遺伝的多型は消化器がんのリスクファクターである"日本がん検診・診断学会誌. 10・2. 61-63 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita: "Rapid quantification of DNase I activity in 1-mL serum samples using a cellulose acetate membrane and SYBR Green I"Clin.Chem.. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 安国年博: "ヒトDNase Iの遺伝子内に見出されたSNPと疾患との相関の検索"DNA多型. 12(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 坪田悦子: "マウス脳の年齢依存性発現遺伝子"DNA多型. 12(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 金古 康: "イヌDNase Iの精製と遺伝子クローニング"DNA多型. 12(印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, E.Tsubota, M.Aoyama, T.Matsuki, K.Kishi: "Age-related changes of gene expression in mouse kidney : fluorescence differential display-PCR analysis."Mech.Aging Development. 113(2). 135-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, E.Nakazato, Y.Nakashima, K.Mogi, K.Kishi: "The molecular basis for genetic polymorphism of human deoxyribonuclease II(DNaseII) : a single nucleotide substitution in the promoter region of human DNase II gene changes the promoter activity."FEBS Lett.. 467(1/2). 231-234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, K.Mogi, T.Yasuda, T.Nakajima, Y.Nakashima, S.Mori, T.Hoshino, K.Kishi: "Mammalian deoxyribonuclease I are classified into three types : pancreas, parotid and pancreasparotid (mixed) types, based on differences in tissue concentrations."Biochem.Biophys.Res.Commun.. 269(2). 481-484 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakajima, T.Yasuda, H.Takeshita, Y.Nakashima, S.Mori, K.Mogi, K.Kishi: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies."Exp.Clin.Immunogenet. 17(2). 71-76 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, S.Mori, T.Yasuda, K.Mogi, T.Nakajima, E.Nakazato, S.Tsutsumi, K.Fujikawa, Y.Kaneko, R.Iida, K.Kishi: "Japanese population data for two STR loci, HumTPO and HumLPL."Legal Med.. 2(2). 64-67 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Tsutsumi, H.Takeshita, T.Yasuda, H.Kuwano, K.Kishi: "Association of the DNase I phenotype 2 with colorectal carcinoma risk in Japanese populations."Cancer Lett.. 159(1). 109-112 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, 0.Hosomi, H.Takeshita, T.Nakajima, Y.Nakashima, S.Mori, Y.Hanaoka, K.Kishi: "Molecular cloning of cDNA encoding Xenopus laevis deoxynibonuclease I."DNA Sequences. 11(3/4). 247-255 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, S.Mori, K.Mogi, K.Kishi: "The life span of DNASE1*6 gene product is shorter than that of other DNase I gene products."DNA Polymorphism. 8. 207-210 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, T.Matsuki, T.Yasuda, K.Kishi: "Identification of novel genes expressed differentially in mouse kidney during aging."DNA Polymorphism. 8. 123-126 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, K.Mogi, T.Yasuda, S.Mori, Y.Nakashima, T.Nakajima, H.Akuzawa, S.Nakajo, Y.Hirota, K.Kishi: "Postmortem absorption of dichioromethane : a case study and animal experiments."Int.J.Legal Med.. 114(1/2). 96-100 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, M.Ueki, R.Iida, T.Nakajima, S.Mori, K.Mogi, Y.Kaneko, K.Kishi: "Tissue-specific in vivo inhibition of DNase I gene expression by somatostatin."Biochem.Biophys.Res.Commun.. 283(2). 287-291 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Mori, T.Yasuda, H.Takeshita, T.Nakajima, E.Nakazato, K.Mogi, Y.Kaneko, K.Kishi: "Molecular, biochemical and immunological analyses of porcine pancreatic DNase I."Biochim.Biophys.Acta. 1547(2). 275-287 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, Y.Nakashima, K.Mogi, K.Kishi, H.Shiono, K.Sagisaka, I.Yuasa, H.Nishimukai, H.Kimura: "Geographical north-south decline in DNase I allele 2 in Japanese population."Hum.Biol.. 73(1). 129-134 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, E.Tsubota, T.Matsuki, K.Kishi: "Cloning, mapping, genomic organization and expression of mouse M-LP, a new member of the peroxisomal membrane protein Mpv 17 domain family."Biochem.Biophys.Res.Commun.. 283(2). 292-296 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, R.Iida, T.Nakajima, S.Mori, K.Mogi, Y.Kaneko, K.Kishi: "Amphibian DNase I are characterized by a C-terminal end with a unique, cystein-rich stretch and by the insertion of a serine residue into the Ca^<2+>-binding site."Biochem.J.. 357(2). 473-480 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, T.Matsuki, T.Yasuda, K.Kishi: "Age-dependent expression of genes in mouse kidney."DNA Polymorphism. 9. 59-63 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, S.Mori, T.Nakajima, K.Mogi, Y.Kaneko, K.Kishi, T.Yasuda: "The STR loci HumTPO and HumLPL : data of 7 Japanese populations."DNA Polymorphism. 9. 178-181 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, E.Nakazato, T.Nakajima, S.Mori, K.Mogi, Y.Kaneko, R.Iida, K.Kishi: "Use of human recombinant DNase I expressed in COS-7 cells as an immunogen to produce a specific anti-DNase I antibody."Exp.Clin.Immunogenet.. 18(4). 226-232 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Kishi, T.Yasuda, H.Takeshita: "DNase I : structure, function, and use in medicine and forensic science."Legal Med.. 3(2). 69-83 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, E.Tsubota, T.Matsuki: "Identification and characterization of two novel human polymorphic STRs on Y chromosome."Int.J.Legal Med.. 15(1). 54-56 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, T.Yasuda, T.Nakajima, S.Mori, K.Mogi, H.Ohkawara, M.Nara, K.Kishi: "A death resulting from inadvertent intravenous infusion of external feed."Int.J.Legal Med.. 116(1). 36-38 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, K.Kishi: "Activity staining for detection of nibonucleases using the dried agarose film overlay(DAFO)method after isoelectnic focusing."Methods in Enzymology. 341. 94-112 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] S.Tsutsumi, Y.Kaneko, T.Asao, H.Kuwano, S.Kudo, H.Takeshita, T.Yasuda, K.Kishi: "DNase I is present in the chief cells of human and rat stomach."Histochem.J.. 33(9/10). 531-535 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, K.Mogi, Y.Kaneko, M.Ueki, R.Iida, K.Kishi: "Abrupt pubertal elevation of DNase I gene expression in human pituitary glands of both sex."FEBS Lett.. 510(1). 22-26 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakajima, T.Yasuda, H.Takeshita, S.Mori, K.Mogi, Y.Kaneko, E.Nakazato, K.Kishi: "Production and characterization of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine."Biochim.Biophys.Acta. 1570(3). 160-164 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, E.Tsubota, K.Sawazaki, M.Masuyama, T.Matsuki, T.Yasuda, K.Kishi: "Characterization and haplotype analysis of the polymorphic Y-STRs DYS443, DYS444 and DYS445 in a Japanese population."Int.J.Legal Med.. 116(3). 191-194 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, E.Tsubota, H.Takatsuka, M.Masuyama, T.Matsuki, K.Kishi: "Five age-dependently expressed genes in mouse brain revealed by the fluorescence differential display-PCR technique."Exp.Gerontol.. 37(8/9). 1121-1126 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Mogi, H.Takeshita, T.Yasuda, T.Nakajima, Y.Kaneko, R.Iida, K.Kishi: "Molecular evolution of vertebrate DNase I amphibian DNase I are characterized by a C-terminal end with a unique, cystein-rich stretch."DNA Polymorphism. 10. 88-92 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, E.Tsubota, M.Masuyama, H.Takatsuka, T.Matsuki, T.Yasuda, K.Sawazaki, T.Yamada, Y.Ogata, K.Kishi: "Identification and haplotype analyses of 5 novel polymorphic Y-STRs."DNA Polymorphism. 10. 160-163 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, H.Takatsuka, E.Tsubota, M.Masuyama, T.Matsuki, T.Yasuda, K.Kishi: "Subcellular localization and physiological function of M-LP, the age-dependently expressed molecule in mouse kidney."DNA Polymorphism. 11. 113-117 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakajima, T.Yasuda, H.Takeshita, K.Mogi, Y.Kaneko, M.Ueki, R.Iida, K.Kishi: "A single nucleotide polymorphism(A-75G) in the proximal promoter region causes the genetic polymorphism for human deoxyribonuclease II."DNA Polymorphism. 11. 230-234 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, R.Iida, H.Takeshita, M.Ueki, T.Nakajima, Y.Kaneko, K.Mogi, T.Tsukahara, K.Kishi: "A simple method of DNA extraction and STR typing from urine samples using a commercially available DNAIRNA extraction kit."J.Forensic Sci.. 48(1). 108-110 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, T.Nakajima, K.Mogi, Y.Kaneko, R.Iida, K.Kishi: "A single amino acid substitution ofLeul3OIle in snake DNase I contributes to the acquisition of thermal stability : a clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates."Eur.J.Biochem.. 270(2). 307-314 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, T.Yasuda, E.Tsubota, H.Takatsuka, M.Masuyama, T.Matsuki, K.Kishi: "M-LP, Mpv17-like protein, has a peroxisomal membrane targeting signal comprising a transmembrane domain and a positively charged loop and up-regulates expression of the manganese superoxide dismutase gene."J.Biol.Chem.. 278(8). 6301-6306 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Nakajima, T.Yasuda, H.Takeshita, K.Mogi, Y.Kaneko, K.Kishi: "Production of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine."Legal Med.. 5(Suppl.). s214-s216 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Mogi, H.Takeshita, T.Yasuda, T.Nakajima, B.Nakazato, Y.Kaneko, M.Itoi, K.Kishi: "Carp hepatopancreatic DNase I : biochemical, molecular, and immunological properties."J.Biochem.. 133(3). 377-386 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda, H.Takeshita, M.Ueki, T.Nakajima, K.Mogi, Y.Kaneko, R.Iida, K.Kishi: "Usefulness of deoxyribonuclease I(DNase I)polymorphism for individualization from small aged urine stains."Legal Med.. 5(2). 105-107 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] R.Iida, K.Sawazaki, H.Ikeda, T.Miyamoto, E.Tsubota, H.Takatsuka, M.Masuyama, T.Matsuki, T.Yasuda, K.Kishi: "A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444 and DYS445."J.Forensic Sci.. 48(5). 1088-1090 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Yoshida, Y.Kawai, K.Masamura, M.Katoh, N.Tanaka, M.Saga, I.Miyamori, T.Yasuda, H.Tada, S.Ito, H.Hoshizaki, S.Oshima, K.Taniguchi: "An abrupt elevation of the serum deoxyribonuclease I(DNase I)activity in acute myocardial infarction."Circulation J.. 67(Suppl.). 300 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Mogi, H.Takeshita, T.Yasuda, M.Ogura, S.Nakajo, K.Kishi: "Case report : food poisoning to death by Podostroma cornu-damae, its case history and autopsy findings."Acta Crim.Japon. 69(1). 14-20 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Kaneko, H.Takeshita, K.Mogi, T.Nakajima, T.Yasuda, M.Itoi, H.Kuwano, K.Kishi: "Molecular, biochemical and immunological analyses of canine pancreatic DNase I."J.Biochem.. 134(5). 711-718 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] H.Takeshita, T.Nakajima, K.Mogi, Y.Kaneko, T.Yasuda, R.Iida, K.Kishi: "Rapid quantification of DNase I activity in 1-mL serum samples using a cellulose acetate membrane and SYBR Green I."Clin.Chem.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Yasuda: "A simple method of DNA extraction and STR typing from urine samples using a commercially available DNA/RNA extraction kit"J.Forensic Sci.. 48・1. 108-110 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yasuda: "A single amino acid substitution of Leu130Ile in snake DNase I contributes to the acquisition of thermal stability"Eur.J.Biochem.. 270・2. 307-314 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] R.Iida: "M-LP, Mpv17-like Protein, Has a peroxisomal membrane targeting signal comprising a transmembrane domain and a positively charged loop and up-regulates expression of the manganese superoxide dismutase gene"J.Biol.Chem.. 278・8. 6301-6306 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Nakajima: "Production of murine monoclonal anti-human DNase II antibodies, and their use for immunoaffinity purification of DNase II from human liver and urine"Legal Med.. 5・Suppl.1. s214-s216 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Mogi: "Carp hepatopancreatic DNase I"J.Biochem.. 133・3. 377-386 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Yasuda: "Usefulness of deoxyribonuclease I (DNase I ) polymorphism for individualization from small aged urine stains"Legal Med.. 5・2. 105-107 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] R.Iida: "A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444 and DYS445"J.Forensic Sci.. 48・5. 1088-1090 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Yoshida: "An abrupt elevation of the serum deoxyribonuclease I (DNase I ) activity in acute myocardial infarction"Circulation J.. 67・Suppl.1. 300 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Mogi: "Case report : food poisoning to death by Podostroma cornu-damae, its case history and autopsy findings"Acta Crim.Japon. 69・1. 14-20 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Kaneko: "Molecular, biochemical and immunological analyses of canine pancreatic DNase I"J.Biochem.. 134・5. 711-718 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Takeshita: "Rapid quantification of DNase I activity in 1-mL serum samples using a cellulose acetate membrane and SYBR Green I"Clin.Chem.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 中島たみ子: "ヒトDNaseIIの多型はプロモーター領域のSNP(A-75G)によって発生した"DNA多型. 11. 230-234 (2003)

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      2003 Annual Research Report
  • [Publications] 飯田礼子: "マウス腎年齢依存性発現分子M-LPの細胞内局在と生理的機能"DNA多型. 11. 113-117 (2003)

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      2003 Annual Research Report
  • [Publications] 岸紘一郎: "DNase Iの遺伝的多型は消化器がんのリスクファクターである"日本がん検診・診断学会誌. 10・2. 61-63 (2003)

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      2003 Annual Research Report
  • [Publications] 安田年博: "ヒトDNase Iの遺伝子内に見出されたSNPと疾患との相関の検索"DNA多型. 12(印刷中). (2004)

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      2003 Annual Research Report
  • [Publications] 坪田悦子: "マウス脳の年齢依存性発現遺伝子"DNA多型. 12(印刷中). (2004)

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      2003 Annual Research Report
  • [Publications] 金古 康: "イヌDNase Iの精製と遺伝子クローニング"DNA多型. 12(印刷中). (2004)

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      2003 Annual Research Report
  • [Publications] Tamiko Nakajima: "Production and characterization of murine monoclonal anti-human DNase II antibodies, and these use for immunoaffinity purification of DNase II from human liver and urine"Biochimica et Biophysica Acta. 1570・3. 160-164 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Reiko Iida: "Five age-dependently expressed genes in mouse brain revealed by the fluorescence differential display-PCR technique"Experimental Gerontology. 37・8-9. 1121-1126 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Abrupt pubertal elevation of DNase I gene expression in human pituitary glands of both sexes"FEBS Letters. 510・1. 22-26 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Haruo Takeshita: "A death resulting from inadvertent intravenous infusion of exteral feed"International Journal of Legal Medicine. 116・1. 36-38 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Reiko Iida: "Characterization and haplotype analysis of the polymorphic : Y-STRs DYS443, DYS444 and DYS445 in a Japanese population"International Journal of Legal Medicine. 116・3. 191-194 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Toshihiro Yasuda: "A simple method of DNA extraction and STR typing from urine samples using a commercially available DNA/RNA extraction kit"Journal of Forensic Sciences. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Reiko Iida: "M-LP, Mpv17-like protein, has a peroxisomal membrane targeting signal comprising a transmembrane domain and a positively charged loop and up-regulates expression of the manganese superoxide dismutase gene"Journal of Biological Chemistry. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Toshihiro Yasuda: "A single amino acid substitution of Leu 130 lle in snake DNase I contributes to the acquisition of thermal stability : a clue to the molecular evolutionary mechanism of cold-blooded to warm-blooded vertebrates"European Journal of Biochemistry. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Kouichi Mogi: "Carp hepatopancreatic DNase I : biochemical, molecular and immunological properties"Journal of Biochemistry. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Reiko Iida: "A novel multiplex PCR system consisting of Y-STRs, DYS441, DYS442, DYS443, DYS444 and DYS445"Journal of Forensic Sciences. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] 茂木 康一: "脊椎動物DNase Iの分子進化"DNA多型. 10. 88-92 (2002)

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      2002 Annual Research Report
  • [Publications] 飯田 礼子: "Y染色体上の新規多型性STR5種の同定とハプロタイプ解析"DNA多型. 10. 160-168 (2002)

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      2002 Annual Research Report
  • [Publications] 茂木 康一: "カエンタケ誤食による食中毒死例"犯罪学雑誌. (印刷中).

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  • [Publications] Haruo Takeshita: "Geographical north-south cline in DNase I allele 2 in Japanese populations"Human Biology. 73(1). 129-134 (2001)

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      2001 Annual Research Report
  • [Publications] Shinjiro Mori: "Molecular, biochemical and immunological analyses of porcine pancreatic DNase I"Biochimica et Biophysica Acta. 1547(2). 275-287 (2001)

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      2001 Annual Research Report
  • [Publications] Reiko Iida: "Cloning, mapping, genomic organization and expression of mouse M-LP, a new member of the peroxisomal membrane protein Mpv17 domain family"Biochemical and Biophysical Research Communications. 283(2). 292-296 (2001)

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      2001 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Tissue-specific in vivo inhibition of DNase I gene expression by somatostatin"Biochemical and Biophysical Research Communications. 283(2). 287-291 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Amphibian DNases I are characterized by a C-terminal end with a unique, cystein-rich stretch and by the insertion of a serine residue into the Ca^<2+>-binding site"Biochemical Journal. 357(2). 473-480 (2001)

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      2001 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Activity staining for detection of ribonucleases using dried agarose film overlay method after isoelectric focusing"Methods in Enzymology. 341. 94-112 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Abrupt pubertal elevation of DNase I gene expression in human pituitary glands of both sexes"FEBS Letters. 510(1). 22-26 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Haruo Takeshita: "Use of human recombinant DNase I expessed in COS-7 cells as an immunogen to produce a specific anti-DNase I antibody"Experimental and Clinical Immunogenetics. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Reiko Ikeda: "Novel polymorphic Y-STRs DYS441, DYS442 and DYS443 : characterization and haplotype analysis"International Journal of Legal Medicine. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Haruo Takeshita: "A death resulting from inadvertent intravenous infusion of exteral feed"International Journal of Legal Medicine. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Koichiro Kishi: "DNase I : structure, function, and use in medicine and forensic science"Legal Medicine. 3(2). 69-83 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 飯田 礼子: "マウス腎の年齢依存性発現遺伝子"DNA多型. 9. 59-63 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 竹下 治男: "STR座位HumTPOおよびHumLPL:日本人7集団のデータについて"DNA多型. 9. 178-181 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Souichi Tsutsumi: "DNase I is present in the chief cells of human and rat stomachs"Histochemical Journal. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Reiko Iida: "Identification and characterization of two novel human polymorphic STRs on the Y chromosome"International Journal of Legal Medicine. 115(2). 54-56 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 岸 紘一郎: "尿 DNase I"生物物理化学. 45. 55-59 (2001)

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      2001 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Molecular cloning of cDNA encoding Xenopus laevis deoxyribonuclease I"DNA Sequence. 11(3/4). 247-255 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tamiko Nakajima: "Rapid purification of human DNase I using mouse monoclonal anti-DNase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. 17(2). 71-76 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Reiko lida : "Age-related changes of gene expression in mouse kidney Fluorescence differential display-PCR analysis"Mechanisms of Aging and Development. 113(2). 133-142 (2000)

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      2000 Annual Research Report
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (DNase II)"FEBS Letters. 467(1/2). 231-234 (2000)

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      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Mammalian deoxyribonuclease I are classified into three types"Biochemical and Biophysical Research Communications. 269(2). 481-484 (2000)

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      2000 Annual Research Report
  • [Publications] Souichi Tsutsumi: "Association of DNase I phenotype 2 with colorectal carcinoma risk in Japanese populations"Cancer Letters. 159(1). 109-112 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Geographical north-south decline in DNase I allele 2 in Japanese populations"Human Biology. 73(1). 129-134 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Japanese population data for two STR loci, HumTPO and HumLPL"Legal Medicine. 2(2). 64-67 (2000)

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      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane : a case study and animal experiments"International Journal of Legal Medicine. 114(1/2). 96-100 (2000)

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      2000 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Activity staining for detection of ribonulceases by means of the dried agarose film overlay method after electrophoresis"Methods in Enzymology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Haruo Takeshita: "A death resulting from inadvertent intravenous infusion of exteral feed"International Journal of Legal Medicine. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Koichiro Kishi: "DNase I : structure, function, and use in medicine and forensic science"Legal Medicine. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 竹下治男: "DNASE1^*6遺伝子のつくる酵素の寿命は短い"DNA多型. 8卷. 207-210 (2000)

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      2000 Annual Research Report
  • [Publications] 飯田礼子: "年齢依存性発現を示すマウス腎の新規遺伝子検索"DNA多型. 8卷. 123-126 (2000)

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      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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