| Project/Area Number |
12357013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIMAZAKI Yoshihiro (2001-2003) Kyushu University, Faculty of Dental Science, Assistant, 大学院・歯学研究院, 助手 (10291519)
古賀 敏比古 (2000) 九州大学, 歯学研究院, 教授 (10037541)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Yutaka Kyushu University, University Farm, Faculty of Agriculture, Assistant, 農学部附属農場, 助手 (20243925)
OKANO Kaoru Kyushu University, University Farm, Faculty of Agriculture, Assoc.Prof., 農学部附属農場, 助教授 (40038328)
OHO Takahiko Kyushu University, University Hospital, Assistant Prof., 講師 (50160940)
嶋崎 義浩 九州大学, 歯学研究院, 助手 (10291519)
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| Project Period (FY) |
2000 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥40,900,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2003: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2002: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2001: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2000: ¥13,600,000 (Direct Cost: ¥13,600,000)
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| Keywords | Dental caries / Milk / Cariogenic bacteria / Colonization Factor / う触 / う触細菌 / ラ蝕 / ラ細菌 |
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| Research Abstract |
Cell surface Protein antigen(PAc) and water-insoluble glucan-synthesizing enzyme(GTF-I) produced by cariogenic Streptococcus mutans are two major factors implicated in the colonization of the human oral cavity by this bacterium. We examined the effect of bovine milk, produced after immunization with a fusion protein of functional domains of these proteins, on the recolonizaion of S.mutans. To prerare immune milk, a pregnant Holstein cow was immunized with the fusion protein PAcA-GB, a fusion of the saliva-binding alanine-rich region(PAcA) of PAc and the glucan-binding(GB) domain of GTF-I. After eight adult subjects received cetylpyridinium chloride(CPC) treatment, one subgroup(n=4) rinsed their mouths with immune milk arid a control group(n=4) rinsed with nonimmune milk. S.mutans levels in saliva and dental plaque decreased after CPC treatment in both groups. Mouth rinsing with immune milk significantly inhibited recolonization of S.mutans in saliva and plaque. On the other hand, the n
… More
umber of S.mutans cells in saliva and plaque in the control group increased immediately after the CPC treatment and surpassed the baseline level 42 and 28 days, respectively, after the CPC treatment. The ratios of S.mutans to total streptococci in saliva and plaque in the group that received immune milk were lower than those in the control group. These results suggest that milk produced from immunized cow may be useful for controlling S.mutans in the human oral cavity.
We examined the effect of the immune milk on the cariogenicity of S.mutans in a rat model. Concentrated immune milk was fed to rats once a day for 55 days. The group that received immune milk had significantly less caries development than controls.
The bovine lactoferrin molecule and relatively long lactoferrin fragments containing residues 473 to 538 strongly inhibited adherence of S.mutans to saliva-coated hydroxyapatite beads. Each cysteine residue in Lf411(residents 473 to 538) was replaced by a serine residue, and the mutants Lf411-C481S and Lf411-C532S strongly inhibited S.mutans adherence. These results suggests that the functional domain of lactoferrin that binds to a salivary film lies in residues 473 to 538 and that region might be concealed by disulfide bond formation between Cys481 and Cys532 in the Lf411 fragment. Less
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