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Confocal laser scan system to image parallel processing of the brain neurons in behaving animals

Research Project

Project/Area Number 12358013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Neuroscience in general
Research InstitutionOsaka University

Principal Investigator

ODA Yoichi  Osaka University, Graduate School of Frontier Biosciences, Associate Professor, 大学院・生命機能研究科, 助教授 (00144444)

Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥35,670,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2002: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2001: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2000: ¥20,200,000 (Direct Cost: ¥20,200,000)
Keywordsconfocal laser scan system / calcium imaging / zebrafish / Mauthner cell / reticulospinal neurons / inhibitory synaptic response / escape response / 共焦点レーザスキャン / 光学測定 / ゼプラフィッシュ / 後脳 / 並列処理 / ゼブラフィシュ / 網様体脊髄ニューロン / ニューロン群活動 / カルシウムイオン
Research Abstract

We developed two confocal laser systems to image neuronal activities of behaving animals. The first system consists of a high-speed scanner built on an upright microscope. It can scan 500 frames per second but has a low signal-to-noise ratio to detect faint change in fluorescence signals. The second system is based on a conventional confocal laser system, but the scanning speed was improved to collect 10 full-frames to 500 line-frames every second. This confocal system has a higher signal-to-noise ratio to detect fluorescence change associated with synaptic inputs without averaging them. Using the system we observed the activities of reticulospinal neurons in the hindbrain of zebrafish larvae. Since the body and brain of larval zebrafish are transparent, the neuronal activities in vivo can be observed by calcium imaging of the neurons in the intact brain. Especially we succeeded in in vivo imaging of functional inhibitory networks on the Mauthner cell which is known to initiate fast escape response behavior of fish. It is the first report of imaging inhibitory synaptic action in intact animals. Finally we developed the optical system to observe neuronal activities of behaving animals. For this purpose, the high-speed video camera system was added to the laser-scan system to measure the fluorescence responses of neurons and behavioral responses of zebrafish simultaneously. The high-speed confocal laser scanning system associated with motion detector will help us to understand which and how central neurons contribute to controlling the simple behavior of animals.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Komatsu, H.: "Effects of enflurane on the long-term potentiation in the hippocampus"Prog. Anesth. Mech.. 6. 515-518 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, M.: "In vivo imaging of functional inhibitory networks on the Mauthner cell of larval zebrafish"J. Neurosci.. 22. 3929-3939 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小田洋一: "学習を担うシナプス伝達の長期抑制"蛋白質核酸酵素(増刊号)「神経回路形成と機能発達」. 464-473 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小田洋一: "記憶のメカニズム"脳21. 3. 258-264 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小田洋一: "学習・記憶を担うシナプス長期増強"生物物理. 41. 80-85 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小田洋一: "聴覚 脳・神経入門講座(渡辺雅彦編)下巻 pp.117-130"羊土社. (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Komatsu,H.: "Effects of enflurane on the long-term potentiation in the hippocampus"Prog. Anesth. Mech.. 6. 515-518 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi,M.: "In vivo imaging of functional inhibitory networks on the Mauthner cell of larval zebrafish"J. Neurosci.. 22. 3929-3939 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi M.: "In vivo imaging of functional inhibitory neworks on the mauthner cell of larval zebrafish"J. Neurosci.. 22. 3929-3939 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakayama H.: "Intrinsic and extrinsic mechanisms underlying heterogeneous excitability of segmentally homologous reticulospinal neurons in teleost hindbrain"Neurosci. Res.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kohashi T.: "Imaging of segmentally homologous reticulospinal neurons in zebrafish hindbrain activated during escape behaviors"Neurosci. Res.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小田洋一: "聴覚 脳・神経入門講座(渡辺雅彦編)下巻"羊土社. 117-130 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, M.: "In vivo imaging of functional inhibitory neworks on the mauthner cell of larval zebrafish"J. Neurosc.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小田洋一: "逃避運動の制御と学習を担うマウスナー細胞。In:魚類のニューロサイエンス-魚類神経科学研究の最前線-pp. 22-37"恒星社厚生閣. 310 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Komatsu: "Effects of enflurane on the long-term potentiation in the hippocampus."Prog.Anesth.Mech.. 6. 515-518 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Takahashi: "Functional development of the recurrent inhibitory network of the Mauthner cell in zebrafish larvae."Neurosci.Res.. S130. 130 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Nakayama: "Functional connections among segmentally arranged reticulospinal neurons in the hindbrain."Neurosci.Res.. S130. 149 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小田洋一: "記憶の神経メカニズム"脳21. 3. 122-128 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小田洋一: "学習を担うシナプス伝達の長期増強"蛋白質・核酸・酵素 増刊号「神経回路形成と機能発達」. 464-473 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小田洋一: "学習・記憶をになうシナプス長期増強"生物物理. 41(印刷中). (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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